Method for separating and enriching vascular endothelial progenitor cells from peripheral blood

A technology for the separation and enrichment of endothelial progenitor cells, which is applied in the field of vascular cell separation, can solve the problems of large differences in separation efficiency, proliferation potential, and unstable results of separation cells, and achieve less miscellaneous cells, high separation efficiency, and good separation effect Effect
CN105695395AInactive Publication Date: 2016-06-22SHANDONG MEIJIA SAIPEI BIOLOGICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG MEIJIA SAIPEI BIOLOGICAL TECH CO LTD
Publication Date
2016-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of vascular cell separation, in particular to a method for separating and enriching vascular endothelial progenitor cells from peripheral blood. The method comprises the steps of obtaining a peripheral blood sample, and using PBS phosphate buffer for dilution; adding diluted blood into a hydroxyethyl starch solution, performing centrifuging and sucking monocular cell sap of a middle albuginea cell layer; using the PBS phosphate buffer for dilution, performing centrifuging, abandoning a supernatant, and performing resuspension on residues through a nutrition solution; performing culture transferring. The method is high in separation efficiency, adopts few parenchyma cells, only needs a small number of experiment instruments, is good in separation effect and has the repeatability.
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Description

technical field

[0001] The invention relates to the technical field of vascular cell separation, in particular to a method for separating and enriching vascular endothelial progenitor cells from peripheral blood. Background technique

[0002] EPC (endothelial progenitor cells, vascular endothelial progenitor cells) is a kind of precursor cells that can circulate, proliferate and differentiate into vascular endothelial cells, but have not yet expressed the phenotype of mature vascular endothelial cells, nor have they formed blood vessels. The physiological morphology of the cells cultured in vitro is a cobblestone-like endothelial cell layer, and the general surface markers identified by EPC are: CD45(-), CD34(+), KDR(+), CD31(+).

[0003] After the identification and characterization of EPC cell populations derived from peripheral blood, angiogenesis in adults has also been reported. A large number of literature reports are emphasizing the importance of EPC to maintain the ...

Claims

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