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A kind of method for cultivating fungus residue to promote the production of conidia by Trichosporum sinensis

A technology of Chinese hairy spores and conidia, which is applied in the field of microorganisms to achieve the effects of short cycle, shortened induction time, and increased quantity

Active Publication Date: 2017-12-05
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the document "Research on the production of conidia by different factors on the fungus Cordyceps sinensis" (Li Yuling, "Edible Fungus" 2002 No. 5), which discusses the production of conidia by Chinese hairy sporophytes in different media, different light and different temperatures. The research of spore; Another example is the literature "The impact of different trace elements and growth factors on the yield of Cordyceps sinensis conidia" (Li Lingling, Luo Hechun, Hu Min, "Journal of Chongqing Industry and Trade Vocational and Technical College" 2014 No. 2 period), which was passed in Adding trace elements and amino acid growth factors to the basic culture and formula, studied the effect of different trace elements and growth factors on the yield of Mortierella chinensis, but the number of conidia obtained by using the method in the above literature is not higher than 10 5 Magnitude

Method used

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  • A kind of method for cultivating fungus residue to promote the production of conidia by Trichosporum sinensis
  • A kind of method for cultivating fungus residue to promote the production of conidia by Trichosporum sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Medium formula (mass percentage): silkworm chrysalis powder 0.5%, peptone 1%, yeast powder 2%, glucose 2%, anhydrous magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, zinc sulfate heptahydrate 0.0013%, aspartic ammonia Acid 0.1%, vitamin C 0.1%, pure milk 8%.

[0029] Collect the wild fresh Cordyceps sinensis ascospores, pick the monoascospores through the stereoscope and cultivate them, obtain the test tube slope of the Trichospores monospore colony after purification and culture; And pick a few pieces of 0.5cm 2 Bacterial blocks of about the size are inoculated in the medium that can obtain a large amount of mycelium, and the bacterial blocks are evenly crushed with a sterile homogenizer, and cultured in a constant temperature shaker at 120 rpm at 20°C for 10 days, which is recorded as a first-class shaker flask , the primary shake flask was expanded and cultivated under the same culture conditions for 5 days, which was recorded as the secondary shake fla...

Embodiment 2

[0032] Medium formula (mass percentage): silkworm chrysalis powder 0.5%, peptone 1.5%, yeast powder 1%, glucose 3%, anhydrous magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, zinc sulfate heptahydrate 0.0013%, aspartic acid Acid 0.1%, vitamin C 0.1%, pure milk 8%.

[0033]Collect the wild fresh Cordyceps sinensis ascospores, pick the monoascospores through the stereoscope and cultivate them, obtain the test tube slope of the Trichospores monospore colony after purification and culture; And pick a few pieces of 0.5cm 2 Bacterial blocks of about the size are inoculated in the medium that can obtain a large amount of mycelium, and the bacterial blocks are evenly crushed with a sterile homogenizer, and cultured in a constant temperature shaker at 120 rpm at 20°C for 10 days, which is recorded as a first-class shaker flask , the primary shake flask was expanded and cultivated under the same culture conditions for 5 days, which was recorded as the secondary shake flask...

Embodiment 3

[0036] Medium formula (mass percentage): silkworm chrysalis powder 0.5%, peptone 1%, yeast powder 2%, glucose 2%, anhydrous magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, zinc sulfate heptahydrate 0.0013%, aspartic ammonia Acid 0.1%, vitamin C 0.1%, pure milk 8%.

[0037] Collect the wild fresh Cordyceps sinensis ascospores, pick the monoascospores through the stereoscope and cultivate them, obtain the test tube slope of the Trichospores monospore colony after purification and culture; And pick a few pieces of 0.5cm 2 Bacterial blocks of about the size are inoculated in the medium that can obtain a large amount of mycelium, and the bacterial blocks are evenly crushed with a sterile homogenizer, and cultured in a constant temperature shaker at 120 rpm at 20°C for 10 days, which is recorded as a first-class shaker flask , the primary shake flask was expanded and cultivated under the same culture conditions for 5 days, which was recorded as the secondary shake fla...

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Abstract

The invention discloses a method for promoting Hirsutella sinensis to produce conidiophores by mushroom dreg culture. The method comprises the four steps of strain selection, mushroom dreg acquisition, mushroom dreg growth and conidiophore induction. According to the method, high-stability high-activity aweto single ascospores capable of fermenting a large amount of mycelia are used as a strain; and the hyphae are subjected to multistage propagation and subjected to photoperiod stimulated culture by using different light sources in the conidiophore induction stage, thereby obtaining the high-yield Hirsutella sinensis conidiophores (1011-1012) within a short time. Thus, the method has important meanings for industrialized development of aweto artificial culture.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for cultivating fungus residues to promote the production of conidia from Mortierella sinensis. technical background [0002] Cordyceps sinensis is a complex of fruiting bodies (fruiting bodies) and larval bodies grown by Cordyceps sinensis infected and parasitized on the larvae of bat moths. It is an edible fungus and is a traditional precious Chinese medicine in my country. It has various effects such as regulating immune system function, anti-tumor, and anti-fatigue. [0003] Wild Cordyceps only grows in alpine meadows, plateau meadows and other alpine vegetation on the Qinghai-Tibet Plateau at an altitude of 3000-5000 meters. Due to over-harvesting in recent years, Cordyceps resources are on the verge of extinction. On the other hand, with the improvement of people's living standards, the demand for Cordyceps sinensis is increasing, and the artificial cultivation of Cor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 李文佳张宗耀李全平吕延华何俊
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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