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Method for separating and preserving Cordyceps militaris spawn

A technology of strain separation and Cordyceps militaris, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of strain degradation and other problems, and achieve the effects of improving vitality, avoiding strain degradation, and avoiding damage

Active Publication Date: 2012-09-26
BEIJING AGRO BIOTECH RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The object of the present invention is to provide a kind of separation and preservation method of Cordyceps militaris at above-mentioned deficiency, in order to overcome the problem of strain degeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] (1), the preparation of polyspore collecting device:

[0028] The polyspore separation device is made of 250-500ML triangular flask. First select high-temperature and high-pressure-resistant polymer materials (such as PP plastics), cut them into square or circular small pieces smaller than the mouth of the Erlenmeyer flask, and then insert pins from one side to fill the whole small pieces (similar to toothbrushes). Then seal the back of the needle with cotton thread to the side where the tampon goes into the bottle. The side of the tampon that enters the bottle mouth is covered with needles like this, and the polyspore collection device is completed.

[0029] (2), the preparation of polyspore carrier adsorption sand:

[0030] Sieve the river sand with 60 mesh, discard large particles and impurities, and then sieve with 80 mesh to remove fine sand. Use a magnet to absorb the iron, put it in a container and soak it with 10% hydrochloric acid, if there are more organic ...

Embodiment 1

[0043] Example 1 Isolation and preservation of ascospores

[0044] (1), the preparation of polyspore collecting device:

[0045] The polyspore separation device is made of 500ML triangular flask. First select the high temperature and high pressure resistant polymer material (PP plastic), cut it into a circular small piece smaller than the mouth of the Erlenmeyer flask, and then insert the pin from one side to fill the whole small piece (similar to a toothbrush). Then use cotton thread to seal the back of the needle on the side where the tampon enters the mouth of the bottle to make a polyspore collection device.

[0046] (2), the preparation of polyspore carrier adsorption sand:

[0047] Sieve the river sand with 60 mesh, discard large particles and impurities, and then sieve with 80 mesh to remove fine sand. Use a magnet to absorb the iron, put it in a container and soak it with 10% hydrochloric acid. After 24 hours, pour off the hydrochloric acid, wash and soak with wate...

example 2

[0059] Experiment time April 2006:

[0060] 1. Strains:

[0061] Cordyceps militaris (purchased from Institute of Microbiology, Chinese Academy of Sciences)

[0062] 2. Culture medium for liquid shake flasks:

[0063] 300 grams of peeled potatoes (boiled juice), 10 grams of glucose, 2 grams of peptone, 0.5 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 1000 ml of water, and the pH is natural.

[0064] 3. Nutrient solution for fruiting body production: soybean 2%, glucose 1%, milk powder 5%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.05%, vitamin B 1 The ratio of 10mg / 1000ml is formulated as a nutrient solution, and the pH is adjusted at 5.6-7.2.

[0065] 4. The culture box made of PP material:

[0066] The height is 10cm, the diameter of the upper bottom is 12cm, and the diameter of the lower bottom is 8cm. 40 grams of rice in each box and 56 grams of nutrient solution are packaged and mixed, sterilized and inoculated.

[0067] 5. My...

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Abstract

The invention provides a method for separating and preserving Cordyceps militaris spawn. The method is that pure ascospores are separated and obtained from ripe ascocarp, and the ascospores are mixed with collection sand and are preserved. The target of Cordyceps militaris to be preserved is ascospores. Different from the traditional preserved fungal cells or conidiospores, sexual spores are preserved in the invention and can reserve the genetic characteristics of the parent to the maximum extent, so spawn degeneration is avoided. Moreover, when in separating the ascospores, the pure ascospores are obtained through the natural ascocarp ejection method, so the damages caused to the spores by the traditional washing method or scraping method are avoided and the activity of the spores is improved.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps militaris cultivation, and relates to a method for maintaining the strain of Cordyceps militaris and its genetic characteristics. Background technique [0002] Cordyceps militaris (L.Fr) Link, also known as Cordyceps militaris, Cordyceps militaris, belongs to Ascomycota, Pyrenomycetes, Sphaeriales, Clavicipitaceae, Cordyceps (Fr.) Link fungi. Cordyceps militaris is the type species of the fungus of the genus Cordyceps, and it is mostly parasitic on the larvae and pupae of various lepidopteran insects in nature. [0003] In recent years, many studies have shown that the chemical composition and pharmacological effects of artificially cultivated Cordyceps militaris are similar to those of Cordyceps sinensis, but the price is far lower than that of Cordyceps sinensis. Therefore, the development and application of Cordyceps militaris has a great potential market. Artificial cultivation of Cordycep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12M1/24C12R1/645
Inventor 邢礼军马荣才刘宝平
Owner BEIJING AGRO BIOTECH RES CENT
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