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Chaetomium globosum and application thereof

A technology of Chaetomium globosum and colony, applied in application, fungicides, fungi, etc., can solve the problem of less species of Chaetomium antagonism, and achieve the effect of improving the rhizosphere microbial community structure of ginseng

Active Publication Date: 2015-06-10
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few types of antagonistic Chaetomium globosum, and a strain of Chaetomium globosum has been isolated from plants and used to control plant diseases, but there is no report on the use of Chaetomium globosum to control fungal diseases of ginseng

Method used

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  • Chaetomium globosum and application thereof
  • Chaetomium globosum and application thereof
  • Chaetomium globosum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Isolation and Preservation of Chaetomium globosum FSR-74 Strain

[0047] The strain was isolated from the rhizosphere soil of the perennial ginseng plant in the elm ginseng cultivation field in Fusong County, Jilin Province. The above soil samples were collected and passed through a 2 mm sieve after removing litter on the surface. Weigh 10 g of fresh soil samples, put them into a conical flask filled with glass beads and 90 mL of sterile saline, shake fully for 30 min, and mix the samples with sterile water evenly to obtain a soil suspension. Take 1 mL of shaking liquid under sterile conditions, add 9 mL of sterile normal saline, and make 10 -2 、10 -3 、10 -4 diluent. 100 μL of each dilution was drawn and added to the potato dextrose agar medium (PDA) plate, uniformly spread by the plate dilution method, each treatment was repeated 3 times, and cultured in a 25 °C incubator for 7 days. After selecting a single colony and transferring it to the potato dextrose agar m...

Embodiment 2

[0053] Inhibitory effect of Chaetomium globosa strain FSR-74 on the growth of pathogens of ginseng fungal diseases

[0054] The inhibitory effect of strain FSR-74 on 7 kinds of fungal disease pathogens of ginseng was determined by the 3-point cup-and-saucer confrontation and the growth rate method: the activated ginseng pathogenic bacteria colonies were made into cakes of corresponding sizes with a puncher with a diameter of 5 mm, and sterile Inoculate to the center of the Potato Dextrose Agar (PDA) plate (diameter 90 mm), insert a bacterial cake of the bacteria to be tested in each of the three sides 2.5 cm away from the pathogenic bacteria, and the other side is blank, as the treatment group, Each treatment was repeated 3 times; in addition, only ginseng pathogenic bacteria were inoculated on a PDA plate, and as a control group, they were placed in a 25 °C incubator and cultured for 7 days. mm), and calculate the bacteriostatic rate according to the following formula. Each ...

Embodiment 3

[0059] Embodiment 3 Chaetomium globosa FSR-74 culture fluid, preparation of spore suspension and fermentation broth

[0060] Activate the slant of Chaetomium globosa FSR-74 test tube, take two 5 mm bacterial cakes and inoculate them in 100 mL potato dextrose liquid medium (PDB) in a 250 mL Erlenmeyer bottle, and cultivate them at 170 r / min, 25 °C After 48 h, the seed liquid was obtained; the seed liquid of Chaetomium globosa FSR-74 was inoculated in the fermentation medium at 10% (volume ratio), and cultivated at 170 r / min, 25 °C for 96 h to obtain the culture liquid; The culture solution of Chaetomium globosa FSR-74 was filtered through two layers of sterile gauze, the filtrate was counted by a hemocytometer, and the spore suspension was dispersed to 6 g L -1 Sodium carboxymethylcellulose (CMC) solution to obtain a spore suspension with a spore content of 2 × 10 5 cfu mL -1 ; The culture solution of Chaetomium globosa FSR-74 strain was centrifuged at 8000 r / mim for 20 min,...

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Abstract

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

Description

technical field [0001] The invention relates to a strain of Chaetomium globosa and its application. Background technique [0002] Ginseng (Panax ginseng C.A. Mey) is a perennial root-negative dicotyledonous medicinal plant of the Araliaceae genus Panax. It is a precious medicinal material in my country and has the reputation of "the king of herbs". Ginseng is widely planted in China, South Korea, North Korea, Russia and other countries. Among them, Northeast China, especially Jilin Province, is the main production area of ​​ginseng and has become one of the important pillar industries there. The long-term artificial planting and variety selection of ginseng is difficult, which leads to the degradation of ginseng germplasm, serious diseases, poor quality and low yield. Extensive use of chemical pesticides leads to pesticide residues and environmental pollution, which reduces the safety and commodity value of ginseng medicinal materials. Disease prevention and control has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 杨利民肖春萍韩梅
Owner JILIN AGRICULTURAL UNIV
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