Hypha coating method for massively producing rice blast germ sexual generations

Abstract

The invention provides a hypha coating method for massively producing rice blast germ sexual generations. The hypha coating method comprises the following steps: (1) preparing hypha suspensions of different mating types of rice blast germs; (2) coating hypha suspension liquid with different mating types on the oat culture medium according to a proportion; and 3) culturing for 5-15 days at the culture temperature of 12-27 DEG C to obtain a large number of rice blast fungus cocysts and ascospores. According to the method, the defects that sexual generation ascocysts and ascospores generated by a confrontation culture method in the prior art are small in total amount, unstable, large in sample difference, inconsistent in sexual offspring maturity and the like are overcome, a large number of rice blast bacteria sexual generations can be obtained within a short time, the formation speed of the ascocysts and the ascospores is high, and the maturity is consistent. The obtained ascocysts and ascospores can be used for quantitative comparison of sexual reproductive capacity of strains, sexual structure observation, nucleic acid extraction and gene expression quantitative analysis.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to a new method, which relates to a method for cultivating and preparing the sexual generation of Magnaporthe grisea. The preparation method is a hypha coating method (Hypha coating, HC method). Background technique [0002] Rice blast is one of the most important diseases of rice, seriously affecting rice yield. Rice blast fungus, also known as rice pear spore, Latin name Pyricularia oryzae, belongs to the kingdom Fungi, Ascomycota, Sordariomycetes, Magnaporthales, Pyriculariaceae, Pyricularia. The rice blast fungus can infect the leaves, stem nodes, branches, panicle necks and grains of rice. In addition to rice, it can also infect more than 50 kinds of gramineous plants such as barley, wheat, and millet. Currently, screening and cultivating disease-resistant rice varieties is the most important measure for rice blast control. However, due to the continuous variation of bla...

AI Technical Summary

Problems solved by technology

This method can produce mature ascothecia and ascospores, but the ascothecia bandwidth is only 2-3mm, and the total amount of ascothecia and ascospores is less, which brings inconvenience to further research, especially molecular biology research, because the useful The amount of sex spores cannot support the sample size required by molecular biology research such as nucleic acid sequencing and proteome analysis

At the same time, the above method also has disadvantages such as instability, large differences between samples, and inconsistent maturity of sexual offspring.

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