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Method for separating parasitic pathogenic fungus

A technology of pathogenic fungi and separation method, which is applied in the field of separation of strong parasitic pathogenic fungi, and can solve the problems of high technical requirements and complicated operation.

Inactive Publication Date: 2014-06-04
QINGDAO KDN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These single spore isolation methods are complicated to operate and require high technical requirements, which limits their application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Acquisition of dry rot spores: Pour 10ml of 1% sterile water agar into a sterile petri dish and cool. Dry rot diseased branches with small black spots were collected from the field, and after the surface was wiped and disinfected with 70% alcohol, the diseased skin was cut and pasted on water agar. Invert the petri dish, place a piece of agar for single spore isolation directly under the diseased skin, with the side of the agar facing the diseased skin. Seal the petri dish and keep it moist at room temperature for 2-12 hours. The ascospores in the mature ascus on the diseased skin will be sprayed onto the water agar sheet, and single spores will be picked under a microscope and transferred to PAD for culture.

Embodiment 2

[0016] The conidia of the dry rot fungus were collected from the dry rot diseased branches with small black spots in the field, and the surface was wiped with 70% alcohol for disinfection. Cut off the infected infected skin, soak it with sterile water, place it in a sterile petri dish, and keep it moist at room temperature (20-25 °C) for 12 hours, and the white and elongated conidia horns can be extruded from the sporozoites. Use a capillary needle to pick the spore horns, spread them on the water agar sheet, pick single spores, and transfer them to PDA for culture.

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PUM

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Abstract

The invention belongs to the field of biotechnology, and provides a method for separating pathogenic fungi, in order to facilitate future research. Single spore isolation is the most effective method for separating parasitic pathogenic fungi, particularly suitable for the separation of pathogenic fungi, which are seriously polluted and difficult to separate by a traditional tissue isolation method, such as Venturia nashicola, apple Marssonina coronaria and Cercospora fungus. The traditional single spore isolation technique has complex operation, high technical requirements, and limited application. Employing capillary with diameter of 1 mm for drilling greatly simplifies the operation process, realizes strong operability of single spore isolation and higher speed; and general technical personnel can grasp the technology through simple training, so as to expand the scope of application. The method is mainly used for the separation of parasitic fungi and ascospores and gains good effect.

Description

technical field [0001] The invention belongs to the field of biological technology, and mainly relates to a method for isolating strong parasitic pathogenic fungi. Background technique [0002] Strong parasitic fungi mainly parasitize on living plants, obtain necessary nutrients from living host tissues, and grow slowly on artificial medium. In addition to pathogenic bacteria, there are also endophytic bacteria, saprophytic bacteria and other miscellaneous bacteria in the host tissue of the disease. Because these miscellaneous bacteria grow quickly on the artificial medium, the separation of strong parasitic fungi by conventional diseased tissue isolation methods is often contaminated by miscellaneous bacteria, and the success rate of isolation is very low. Single spore isolation can avoid contamination caused by miscellaneous bacteria in diseased tissues, and is used for the isolation of strong parasitic fungi with a high success rate. [0003] Single spore isolation is ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 王英俊郝智慧张浩
Owner QINGDAO KDN BIOTECH
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