Ultrasonic-assisted complex enzymatic hydrolysis extraction of Gracilaria polysaccharides and its extraction method

A composite enzymolysis and ultrasonic technology, applied in the direction of fermentation, can solve the problems of high requirements for strong acid extraction equipment, low yield of Jiangli polysaccharide, long extraction time, etc., to shorten the extraction time, high nutritional and health value, and improve the extraction rate. Effect

Active Publication Date: 2021-05-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Generally speaking, the yields of Jiangli polysaccharides in the above-mentioned prior art are still low, and strong acid extraction requires high equipment, and there are problems such as environmental pollution, long extraction time, high energy consumption, low yield, and low efficiency.

Method used

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  • Ultrasonic-assisted complex enzymatic hydrolysis extraction of Gracilaria polysaccharides and its extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take 1.0g of fine-based Gracilaria powder, add 40mL of disodium hydrogen phosphate-citric acid buffer solution prepared at pH = 5.5, stir on a magnetic stirrer to make the powder fully absorb water and mix evenly, and then place it in a place heated to 55°C In the ultrasonic cleaner, set the ultrasonic time to 40min and the ultrasonic power to 150W for ultrasonic treatment.

[0039] After the ultrasonic treatment, according to the dosage of 3wt% compound enzyme, add 1mL bromelain solution (15mg·mL -1 ) and 1mL cellulase solution (15mg·mL -1 ), and then immediately transferred to a constant temperature water bath at 55°C for enzymolysis for 120 minutes. Here bromelain Bromelain, Zhengzhou Jiuting Chemical Products Co., Ltd. (the same source of bromelain used in the following examples); Cellulase CELLUCLAST 1.5L, Novozymes (China) Biotechnology Co., Ltd. (the cellulose used in the following examples) Enzymes from the same source).

[0040] After enzymolysis, transfer t...

Embodiment 2

[0045] Take 1.0g of fine-based Gracilaria powder, add 100mL of disodium hydrogen phosphate-citric acid buffer solution prepared at pH=5.5, stir on a magnetic stirrer to make the powder fully absorb water and mix evenly, and then place it in a place heated to 55°C In the ultrasonic cleaner, set the ultrasonic time to 40min and the ultrasonic power to 150W for ultrasonic treatment.

[0046] After the ultrasonic treatment, according to the dosage of 3wt% compound enzyme, add 1mL bromelain solution (10mg·mL -1 ) and 1mL cellulase solution (20mg·mL -1 ), and then immediately transferred to a constant temperature water bath at 55°C for enzymolysis for 120 minutes.

[0047] After enzymolysis, transfer to a boiling water bath for 20 minutes, then immediately centrifuge at 5000 rpm for 10 minutes, collect the supernatant, add 3 times the volume of absolute ethanol to the supernatant to precipitate the polysaccharide, and then centrifuge. The condition is 3000rpm, centrifuge for 5min,...

Embodiment 3

[0051] Take 1.0g of fine-based Gracilaria powder, add 80mL of disodium hydrogen phosphate-citric acid buffer solution prepared at pH=5.5, stir on a magnetic stirrer to make the powder fully absorb water and mix evenly, and then place it in a place heated to 55°C In the ultrasonic cleaner, set the ultrasonic time to 20min and the ultrasonic power to 150W for ultrasonic treatment.

[0052] After the sonication, add 1mL bromelain solution (12.5mg·mL -1 ) and 1mL cellulase solution (17.5mg·mL -1 ), and then immediately transferred to a constant temperature water bath at 55°C for enzymolysis for 120 minutes.

[0053] After enzymolysis, transfer to a boiling water bath for 20 minutes, then immediately centrifuge at 5000 rpm for 10 minutes, collect the supernatant, add 3 times the volume of absolute ethanol to the supernatant to precipitate the polysaccharide, and then centrifuge. The condition is 3000rpm, centrifuge for 5min, after the centrifugation, collect the ethanol precipita...

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Abstract

The invention relates to a method for extracting Gracilaria spp. polysaccharides through ultrasonic-assisted composite enzymatic hydrolysis; the method takes decolorized Gracilaria spp powder, adds buffer solution, stirs and mixes, and performs ultrasonic treatment; adds compound enzymes for enzymolysis treatment The compound enzyme is bromelain and the second component enzyme, and the second component enzyme is cellulase or pectinase; after the enzymolysis is completed, the enzyme is inactivated in a boiling water bath, and the extract is separated by centrifugation and suction filtration, and collected The supernatant; the extract is subjected to alcohol precipitation, centrifugation, washing, and drying to obtain a fine-based Gracilaria polysaccharide extract. The extraction method of the invention has the advantages of mild conditions, one-step enzymatic hydrolysis, simple operation, short extraction time, high polysaccharide yield and the like, and the obtained fine-based Gracilaria polysaccharide has strong antioxidant capacity and high nutritional and health care value.

Description

technical field [0001] The present invention relates to Gracilaria spp. polysaccharides, in particular to Gracilaria spp. polysaccharides extracted by ultrasonic-assisted composite enzymatic hydrolysis and its extraction method. The Gracilaria spp. polysaccharides obtained by the method of the present invention have good antioxidant activity; Sexual food engineering technology field. Background technique [0002] With the rapid increase in the number of humans and animals on the earth, terrestrial resources are decreasing day by day; to seek the sustainability of human society, it is necessary to engage in the development and research of marine resources. Since 1970, more than 3,000 natural products have been isolated from seaweeds, sponges, coelenterates, molluscs, echinoderms, and microorganisms, many of which have pharmacological activities such as antifungal, antiviral, anticoagulant, and antitumor. The study of marine natural products has become a hot topic at present....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P19/14C08B37/00
CPCC08B37/00C08B37/0003C12P19/04C12P19/14
Inventor 谭相文李秀华余以刚黄志贤陈春应何文博
Owner SOUTH CHINA UNIV OF TECH
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