Preparation method and application of Gracilaria agar oligosaccharide promoting growth of Lactobacillus bulgaricus

A technology of agar oligosaccharides and lactobacilli, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the use of added compounds to stimulate growth, can solve the problem that the number of probiotics in the product does not meet the standard, and achieve reduced heating time, Less investment, better activity effect

Inactive Publication Date: 2016-04-20
FUJIAN PROVINCE LVQI FOOD COLLOID +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the industrial production of probiotic fermented products, there is often a problem that the number of probiotics in the product does not meet the standard

Method used

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  • Preparation method and application of Gracilaria agar oligosaccharide promoting growth of Lactobacillus bulgaricus
  • Preparation method and application of Gracilaria agar oligosaccharide promoting growth of Lactobacillus bulgaricus
  • Preparation method and application of Gracilaria agar oligosaccharide promoting growth of Lactobacillus bulgaricus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: A method for preparing Gracilaria agar oligosaccharide grown by Lactobacillus bulgaricus

[0033] Step 1. Remove impurities from Gracilaria: wash and remove impurities from Gracilaria until there is no sediment and the water sample is clear, dry, add water 30 times the weight of Gracilaria to fully swell Gracilaria, and then heat in a water bath at 80°C 2h;

[0034] Step 2, autoclaving: Carry out autoclave sterilization to the Gracilaria after heating treatment, kill microorganism, autoclave temperature is 121 ℃, sterilization time is 20min;

[0035] Step 3, extruding glue: Squeeze Gracilaria while it is hot, wrap Gracilaria with filter cloth, and use a press to squeeze the filter cloth containing Gracilaria, collect the polysaccharide solution filtered out by extrusion; put the collected polysaccharide The solution is cooled, dried, crushed into granules and bagged for later use, and stored in a cool and dry place to obtain the Gracilaria crude polysacchari...

Embodiment 2

[0041] Example 2: Process flow for improving the viability of Lactobacillus bulgaricus under low temperature storage with agar oligosaccharides with different degrees of polymerization

[0042] Agar oligosaccharides with different degrees of polymerization were prepared using the above method for preparing oligosaccharides, and added to the fermented oligosaccharides to reach OD 600 In the MRS medium of Lactobacillus bulgaricus with a value of 0.8, agar oligosaccharides (DP3, DP5, DP8) with different degrees of polymerization at the same concentration were added to the medium, and the culture conditions were anaerobic environment, 4°C, standing After culturing, samples were taken every 3 days to observe the number of viable bacteria under a microscope, as shown in Table 1.

[0043] Table 1 Agar oligosaccharides with different degrees of polymerization improve the activity of Lactobacillus bulgaricus under low temperature storage

[0044]

[0045] The results showed that th...

Embodiment 3

[0046] Example 3: Process flow for improving the activity of Lactobacillus bulgaricus under low temperature storage with different concentrations of Gracilaria agar oligosaccharides

[0047] Using the above method for preparing oligosaccharides, prepare different concentrations of agar oligosaccharides, and add them to the fermented oligosaccharides to reach OD 600In the MRS medium of Lactobacillus bulgaricus with a value of 1.2, different concentrations of agar oligosaccharides (1g / L, 3g / L, 5g / L, 7g / L, 10g / L) with a degree of polymerization of 3 were added to the medium In , the culture condition is an anaerobic environment, 4°C, static culture, sampling every 3 days to observe the number of viable bacteria under a microscope, as shown in Table 2.

[0048] Table 2 Different concentrations of Gracilaria agar oligosaccharides improve the activity of Lactobacillus bulgaricus under low temperature storage

[0049]

[0050]

[0051] The results showed that under the conditi...

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Abstract

The invention discloses a preparation method of Gracilaria agar oligosaccharide promoting growth of Lactobacillus bulgaricus. The preparation method includes following steps: step 1, removing impurities of Gracilaria: cleaning, removing impurities of Gracilaria, drying, and placing Gracilaria in water bath at 80 DEG C for heating for 2 h; step 2, sterilizing at high temperature: sterilizing at 121 DEG C for 20 min; step 3, squeezing glue: using filter cloth to wrap Gracilaria, squeezing, collecting a polysaccharide solution, cooling, drying, smashing into granules, and bagging the granules for standby use; step 4, performing enzymatic treatment: dissolving cooled coarse polysaccharide in water to prepare a polysaccharide solution of 5g / L in concentration, adding agarase for treatment, performing enzymolysis for 20-1280 min to prepare an oligosaccharide solution, wherein enzyme adding quantity is 15% and enzyme activity is 300 U / mL; step 5, drying: freeze-drying the oligosaccharide solution or vacuum-drying the same to form solid powder to obtain the agar oligosaccharide. The preparation method can be used for Lactobacillus fermentation enterprises and dairy product production enterprises to produce high-activity Lactobacillus products and high-storability dairy products.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method and application of Gracilaria agar oligosaccharide for promoting the growth of Lactobacillus bulgaricus. Background technique [0002] Gracilaria (Gracilaria) is a large perennial red algae belonging to the Rhodophyta. It is one of the coastal algae culture species in Guangdong, Zhejiang and Taiwan in my country. It is mainly used as feed for fish farming and raw materials for food production. Gracilaria agar oligosaccharide is a non-digestible oligosaccharide, which can significantly promote the growth of Lactobacillus bulgaricus, and can inhibit the growth of pathogenic bacteria, spoilage bacteria and other harmful bacteria, can improve human immunity and improve the endocrine system , promote the absorption of nutrients, improve the sub-health state of the human body, treat enteritis, diarrhea, constipation and side effects of drugs caused by bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N1/38C12N1/04C12R1/225
CPCC12P19/14C12N1/04C12N1/38
Inventor 郭东旭肖安风郑毅洪清林林坤城蔡慧农钟晓婷姜泽东倪辉杨秋明
Owner FUJIAN PROVINCE LVQI FOOD COLLOID
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