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Method for reducing content of lignin in peduncle shred of tobacco

A technology of tobacco stems and lignin, applied in the directions of tobacco, tobacco processing, application, etc.

Active Publication Date: 2012-07-04
GUANGDONG GOLDEN LEAF TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the application of biological complex enzymes to treat cut tobacco stems

Method used

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  • Method for reducing content of lignin in peduncle shred of tobacco
  • Method for reducing content of lignin in peduncle shred of tobacco
  • Method for reducing content of lignin in peduncle shred of tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Reducing Lignin Content in Tobacco Shredded Stems

[0079] Take by weighing Yuxi K326 long cut tobacco stalk 30g, sterilize 3min under the condition of power 800-1200W in microwave oven, cool naturally, at room temperature 0.4% by weight lignin degradation compound enzyme aqueous solution (containing Lip, Mnp and Lac ) is sprayed on the surface of the cut tobacco stem raw material, the lignin-degrading compound enzyme is 3.0 g, mixed thoroughly and then placed in a constant temperature and humidity incubator for enzymolysis, and the enzymolysis time is 12 hours at an enzymolysis temperature of 30°C. At the same time, sterile distilled water was used as a control. After enzymatic hydrolysis, heat in a microwave oven at 800w power for 1min to inactivate the enzyme, air-dry naturally until the moisture content is 12% based on the total weight of the tobacco, and equilibrate at a temperature of 22°C and a relative humidity of 60% for 48h. The method described in...

Embodiment 2

[0084] Embodiment 2: Reducing the lignin content in cut tobacco stems

[0085] Weigh 30g of Yuxi K326 long cut tobacco stems, sterilize for 3min under the condition of power 1200W in a microwave oven, cool naturally, and spray 0.6% by weight lignin degradation compound enzyme aqueous solution (containing Lip, Mnp and Lac) at room temperature On the surface of cut tobacco stem raw materials, 9.0 g of the lignin-degrading compound enzyme is mixed thoroughly and placed in a constant temperature and humidity incubator for enzymolysis, and the enzymolysis time is 72 hours at an enzymolysis temperature of 60°C. Bacterial distilled water was used as a control. After enzymatic hydrolysis, heat in a microwave oven at 1200w power for 5 minutes to inactivate the enzyme, and air-dry it naturally so that its moisture content reaches 12% based on the total weight of enzymatically degraded cut tobacco stems. Equilibrate under conditions for 48h. The lignin content of the shredded tobacco s...

Embodiment 3

[0090] Embodiment 3: reduce the lignin content in the shredded tobacco stem

[0091] Weigh 30g of Yuxi K326 long cut tobacco stems, sterilize for 3min under the condition of power 1000W in a microwave oven, cool naturally, and spray 0.8% by weight lignin degradation compound enzyme aqueous solution (containing Lip, Mnp and Lac) at room temperature On the surface of cut tobacco stem raw material, 6.0 g of the lignin-degrading compound enzyme is mixed thoroughly and placed in a constant temperature and humidity incubator for enzymolysis. The enzymolysis time is 42 hours at an enzymolysis temperature of 45° C. Bacterial distilled water was used as a control. After enzymatic hydrolysis, heat in a microwave oven at 1000w power for 3 minutes to inactivate the enzyme, and air-dry it naturally so that the moisture content reaches 12% based on the total weight of enzymatically degraded cut tobacco stems, at a temperature of 22°C and a relative humidity of 60%. Equilibrate under condit...

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Abstract

The invention relates to a method which adopts lignin-degradation complex enzyme to treat a peduncle shred of tobacco so as to reduce the content of lignin in the peduncle shred of the tobacco. The method comprises the steps of heating and sterilizing the peduncle shred of the tobacco in a microwave oven, degrading the lignin by using the complex enzyme, heating to denature the complex enzyme, equalization treatment and the like. The method disclosed by the invention uses the complex enzyme to treat the peduncle shred of the tobacco so as to reduce the content of the lignin in the peduncle shred of the tobacco, and the tobacco products produced by using the peduncle shred of the tobacco with low content of lignin are obviously improved on the aspects of foreign flavor, smoke strength, irritancy and aftertaste.

Description

【Technical field】 [0001] The invention relates to the technical field of tobacco processing. More specifically, the present invention relates to a method for reducing the lignin content of cut tobacco stems by using a lignin-degrading compound enzyme method to treat cut tobacco stems. 【Background technique】 [0002] With the development of cigarette technology, adding a certain proportion of shredded tobacco stems to cigarette raw materials can partially improve the quality of cigarettes. The main components of shredded tobacco stems are lignin, cellulose and pectin, etc., and lignin is the main substance that causes miscellaneous gas in cut stems, heavy woody gas, and throat burning sensation when burning. Therefore, reducing the lignin content of shredded tobacco stems and improving its application effect is a very key technical problem to solve the quality of these tobacco products. [0003] Lignin is an aromatic polymer compound that strengthens cell walls and binds fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
Inventor 郭春梅张建栋施栩翎
Owner GUANGDONG GOLDEN LEAF TECH DEV
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