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A kind of extraction method of peanut meal polysaccharide

An extraction method and peanut meal technology, which are applied in the field of biochemistry, can solve the problems of strong reaction conditions, environmental pollution, and polysaccharide food safety effects, so as to improve reaction efficiency and conversion rate, improve extraction efficiency, avoid polysaccharide activity and The effect of security breach

Active Publication Date: 2016-02-24
山东金胜生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using strong acid or strong alkali to extract peanut polysaccharides can obtain a higher yield, but due to the strong reaction conditions, it will have a certain impact on the food safety of polysaccharides, and the waste water generated during the production process will cause certain environmental pollution, so It is necessary to develop an efficient and green method for extracting polysaccharides from peanut meal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting peanut meal polysaccharides, comprising the steps of:

[0027](1) Pretreatment: take the raw material of hot-pressed peanut meal, grind it to 500-1000 mesh, add a 70% ethanol solution according to the ratio of solid to liquid (kg / L), and then heat it at a temperature of 30°C and a stirring speed of Under the condition of 150~260r / min, fully stir for 30min, filter with suction, and take the filter residue;

[0028] (2) Enzyme hydrolysis of protein: take the filter residue after pretreatment, add 8 times the weight of culture medium, and prepare a suspension to facilitate the full combination of enzymes, and then add neutral protease Neutralprotease (Novozymes), the amount of enzyme added is according to 0.3g / ml suspension was added, the enzymolysis temperature was 25°C, the pH value was 6, and the enzymolysis time was 2 hours;

[0029] The culture medium is (per liter): (NH 4 ) 2 SO 4 0.3g, FeSO 4 ·7H 2 O0.02g, MnSO 4 ·H 2 O0.03g, MgSO 4 ...

Embodiment 2

[0035] A method for extracting peanut meal polysaccharides, comprising the steps of:

[0036] (1) Pretreatment: take the raw material of hot-pressed peanut meal, grind it to 500-1000 mesh, add a 70% ethanol solution according to the ratio of solid to liquid of 1:10, and then heat it at 40°C with a stirring speed of 150-260r / min. Under the same conditions, fully stir for 30 minutes, filter with suction, and take the filter residue;

[0037] (2) Enzymatic hydrolysis of protein: take the filter residue after pretreatment, add 10 times the weight of culture medium to prepare a suspension to facilitate the full combination of enzymes, and then add neutral protease Neutralprotease (Novozymes), the amount of enzyme added is according to 0.3g / ml suspension was added, the enzymolysis temperature was 30°C, the pH value was 7, and the enzymolysis time was 2 hours;

[0038] The culture medium is (per liter): (NH 4 ) 2 SO 4 0.3g, FeSO 4 ·7H 2 O0.02g, MnSO 4 ·H 2 O0.03g, MgSO 4 0.0...

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PUM

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Abstract

The invention provides a method of extract of peanut meal polysaccharides, including pre-treatment, enzymatic protein, and enzymes: compound enzyme extract polysaccharides, separation and purification, and its composite enzymes are cellulose enzymes, enzyme-soluble, β-hypateThe combination of polylase and amylase, this method effectively removes the protein in it, retains the functional characteristics of polysaccharides to the greatest extent, avoids pollution of organic solvents, improves polysaccharides extraction rate, and improves the purity of polysaccharides.prospect.

Description

[0001] Technical Field The present invention belongs to the technical field of biochemistry, and in particular relates to a method for extracting peanut meal polysaccharides. Background technique [0002] my country is one of the most important major peanut producing countries in the world, with an output of more than 40% of the world's total output. According to statistics, the annual output of my country's peanuts has reached 15.22 million tons, surpassing that of soybeans (15.2 million tons), and has become the first oil crop in my country. The total output, per unit yield and export volume all rank first in the world. In addition to domestic use, a large number of peanuts in my country are exported as raw materials, and the peanut deep-processing industry is relatively weak. Taking Shandong Province, where peanut exports account for 70% of the country, as an example, there are currently 18 specialized peanut food processing plants and 28 production lines; while Japan, whos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 高冠勇陈宁
Owner 山东金胜生物科技有限公司
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