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Method for extracting geniposide acid from folium cortex eucommiae

A technology of geniposide and Eucommia leaves, which is applied in the field of extracting geniposide, can solve the problems of low extraction rate, large energy consumption, and low product purity, so as to improve extraction rate, increase product yield, Facilitate the release effect

Active Publication Date: 2015-12-09
广西优食客食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eucommia ulmoides is a unique economic tree species in my country, with a large distribution area and rich resources. The content of geniposide in Eucommia leaves is 1-4%, which is an ideal raw material for extracting geniposide. Problems such as low extraction rate, low product purity, high energy consumption, large use of organic solvents, large amount of waste water, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting geniposide from leaves of Eucommia ulmoides, specifically comprising the steps of:

[0025] 1) Pre-wet stacking: build a pile of eucommia leaves in a cool place, spray water, and stack them naturally for 6 hours after the water flows out from the bottom of the pile;

[0026] 2) Raw material processing: pulverize the eucommia leaves obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 50°C for 5 minutes, and obtain a paste-like slurry;

[0027] 3) Enzymolysis for the first time: the paste slurry obtained in step 2) is adjusted to pH 4, and 0.05% by weight of the paste slurry is added with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. Part of mannitol was enzymolyzed for 40 minutes to obtain the first enzymolysis solution;

[0028] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 60°C a...

Embodiment 2

[0033] A method for extracting geniposide from leaves of Eucommia ulmoides, specifically comprising the steps of:

[0034] 1) Pre-wet stacking: build a pile of eucommia leaves in a cool place, spray water, and stack them naturally for 8 hours after the water flows out from the bottom of the pile;

[0035] 2) Raw material processing: pulverize the eucommia leaves obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 60°C for 8 minutes, and obtain a paste-like slurry;

[0036] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / ml of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 50 minutes to obtain the first enzymolysis solution;

[0037] 4) Oil removal: add 3 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 70°C and extra...

Embodiment 3

[0042] 1) Pre-wet stacking: build a pile of eucommia leaves in a cool place, spray water, and stack them naturally for 12 hours after the water flows out from the bottom of the pile;

[0043] 2) Raw material processing: pulverize the eucommia leaves obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0044]3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to be 5, add 0.08% by weight of the paste slurry with an activity of 30,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 60 minutes to obtain the first enzymolysis solution;

[0045] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and the sl...

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PUM

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Abstract

The invention discloses a method for extracting geniposide acid from folium cortex eucommiae. The method comprises the following steps of pre-wetting stacking, raw material treating, first-time enzymolysis, deoiling, second-time enzymolysis, column chromatography and drying. According to the method, firstly, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting geniposide from leaves of Eucommia ulmoides. Background technique [0002] Eucommia ulmoides (scientific name Eucommiaulmoides Oliver), also known as Bakelite, is a plant of Eucommiaceae. Eucommia has very few free amino acids, and the small amount of protein it contains is a complete protein similar to most foods, that is, it can be hydrolyzed to detect 8 kinds of amino acids necessary for the human body. The 15 kinds of mineral elements contained in Eucommia were determined, including trace elements such as zinc, copper, and iron, and macroelements such as calcium, phosphorus, potassium, and magnesium. Medicinal eucommia is the dry bark of Eucommia ulmoides, a rare tonic medicinal material in China. It is sweet in taste and warm in nature. It has the effects of nourishing liver and kidney, strengthening tendons and bones, regulatin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 文继承
Owner 广西优食客食品有限公司
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