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Preparation method of abalone viscera protein peptide

A technology for abalone viscera and protein peptides, which is applied in the field of protein peptide preparation and can solve the problems of low yield and purity of abalone protein peptides

Pending Publication Date: 2022-06-17
韩耀辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the disadvantages of low yield and purity of abalone protein peptides obtained when preparing abalone protein peptides using existing methods, and to provide a method for preparing abalone visceral protein peptides, which can obtain high yields and pure protein peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0019] Preparation Example 1: Preparation of Modified Aluminum Hydroxide

[0020] Aluminium hydroxide with a particle size of 5 to 10 microns was placed in a xylene solution of γ-glycidyl ether oxypropyltrimethoxysilane with a concentration of 10 wt %, and the temperature was controlled at 85° C. under stirring for 15 min, and filtered, The obtained solid was calcined at a temperature of 120° C. for 2 hours to obtain modified aluminum hydroxide.

Embodiment 1

[0021] Example 1: Preparation of Abalone Viscera Peptide

[0022] S1. Prepare a saturated saline solution at room temperature. 1 part of abalone viscera (protein content is 11.67%, the same below) was cleaned and then placed in 5 parts of saturated brine for reflux treatment for 2 hours to obtain pretreated abalone viscera.

[0023]S2. Add 1 part of pretreated abalone viscera into 1 part of water and then pulverize it, and add compound enzyme I with a mass of 5‰ of abalone viscera. The compound enzyme I is composed of pancreatin, chymotrypsin, papain, carboxypeptidase and 2-Pentylthiol is composed of a mass ratio of 1:0.1:2:0.5:0.01, and is enzymatically hydrolyzed at a temperature of 60 °C and a pH of 7.0 for 2 hours, and then the pH of the obtained enzymatic hydrolysis product is adjusted to 6.5, A compound enzyme II with a mass of 5‰ of abalone viscera was added. The compound enzyme II was composed of papain, carboxypeptidase and sodium chloride in a mass ratio of 1:0.05:0...

Embodiment 2

[0025] Example 2: Preparation of Abalone Viscera Peptide

[0026] S1. Prepare a saturated saline solution at room temperature. 1 part of abalone viscera (with protein content of 11.67%) was cleaned and then placed in 2 parts of saturated brine for reflux treatment for 1 hour to obtain pretreated abalone viscera.

[0027] S2. Add 1 part of pretreated abalone viscera into 1 part of water and then pulverize it, and add compound enzyme I with a mass of 5‰ of abalone viscera. The compound enzyme I is composed of pancreatin, chymotrypsin, papain, carboxypeptidase and The tert-dodecyl mercaptan is composed of a mass ratio of 1:0.2:5:1.5:0.1, enzymatically hydrolyzed for 10 hours at a temperature of 30 °C and a pH of 9.0, and then the pH of the resulting enzymatic hydrolysis product is adjusted to 6.5 , adding compound enzyme II with a mass of 5‰ of abalone viscera, the compound enzyme II is composed of papain, carboxypeptidase and sodium sulfate in a mass ratio of 1:0.2:0.1, and con...

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Abstract

The invention belongs to the field of protein peptide preparation, and relates to a preparation method of abalone viscera protein peptide, which comprises the following steps: pretreating abalone viscera with saturated salt water, adding water into the pretreated abalone viscera, and crushing; adding a compound enzyme I prepared from pancreatin, chymotrypsin, papain, carboxypeptidase and alkyl mercaptan according to the mass ratio of 1: (0.1-0.2): (2-5): (0.5-1.5): (0.01-0.1) to carry out primary enzymolysis, and adding a compound enzyme II prepared from papain, carboxypeptidase and sodium salt according to the mass ratio of 1: (0.05-0.2): (0.01-0.1) to carry out secondary enzymolysis, and adding a flocculating agent into the obtained enzymatic hydrolysate, carrying out flocculent precipitation by using the flocculating agent containing alginate, gelatin, carrageenan and modified aluminum hydroxide, and carrying out solid-liquid separation. The method provided by the invention can improve the yield and purity of abalone viscera protein peptide, and has great industrial application prospects.

Description

technical field [0001] The invention belongs to the field of protein peptide preparation, in particular to a preparation method of abalone visceral protein peptide. Background technique [0002] Proteins are complex biological macromolecules, which can provide the necessary amino acid components for the metabolism of the organism, ensure the growth of the organism and maintain the energy of life activity. In protein molecules, amino acids are connected to each other according to a certain sequence of amino acids, and with the assistance of "molecular chaperones" and folding enzymes, proteins are folded and coiled to form a stable spatial multi-dimensional structure. In addition to its own functional properties such as emulsifying function, texturizing effect, and gelling effect, protein can also be digested and absorbed by the gastrointestinal tract after being ingested by the human body. Until the 1980s, it was believed that protein was absorbed in the gastrointestinal tra...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/30
CPCC12P21/06C07K1/30
Inventor 韩耀辉
Owner 韩耀辉
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