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Method for extracting huperzine A from huperzia serrata

A technology of huperzine A and Melaleuca tower, which is applied in the field of extracting huperzine A from Melaleuca tower, can solve the problems of short processing time, inconvenient industrialization, and high cost, so as to improve extraction rate, facilitate release, increase The effect on product yield

Inactive Publication Date: 2015-11-18
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zha Shenghua, Li Xiunan, etc. used microwave-assisted extraction and ultrasonic extraction of huperzine A and other methods. Compared with traditional extraction methods, the number of solid-liquid separations is less, the processing time is short, and the operation is simple; the solvent consumption is small and the cost is low; the choice Good performance, less impurities, good energy transmission effect, etc., but the cost is high and it is inconvenient for industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting huperzine A from Melaleuca, specifically comprising the steps of:

[0025] 1) Soaking treatment: Put the Melaleuca pagoda into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 15 hours;

[0026] 2) Raw material treatment: pulverize the Melaleuca tower obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 50°C for 5 minutes, and obtain a paste slurry;

[0027] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 4, add 0.05% by weight of the paste slurry with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 weight portion of manna Carry out enzymolysis with alcohol for 40 minutes to obtain enzymolysis solution;

[0028] 4) Oil removal: add 2 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 60°C and extract for 120 minutes to obtain ...

Embodiment 2

[0034] A method for extracting huperzine A from Melaleuca, specifically comprising the steps of:

[0035] 1) Soaking treatment: Put the Melaleuca pagoda into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 24 hours;

[0036] 2) Raw material treatment: crush the Melaleuca tower obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 60°C for 8 minutes, and obtain a paste slurry;

[0037] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / ml of pectin-cellulose compound enzyme and 0.001% by weight of manna Carry out enzymolysis with alcohol for 50 minutes to obtain enzymolysis solution;

[0038] 4) Oil removal: add 3 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obtain a mixture ...

Embodiment 3

[0044] 1) Soaking treatment: Put the Melaleuca pagoda into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 18 hours;

[0045] 2) Raw material treatment: crush the Melaleuca tower obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste slurry;

[0046] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 5, add 0.08% by weight of the paste slurry with an activity of 30,000 active units / gram of pectin-cellulose compound enzyme and 0.001 weight portion of manna Carry out enzymolysis with alcohol for 60 minutes to obtain enzymolysis solution;

[0047] 4) Oil removal: Add 2 times the weight of purified water to the enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and the slag;

[0048] 5) Aci...

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PUM

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Abstract

The invention discloses a method for extracting huperzine A from huperzia serrata. The method comprises the steps of soaking treatment, raw material treatment, enzymolysis, deoiling, acidolysis, column chromatography and recrystallization. According to the method for extracting the huperzine A from the huperzia serrata, vegetable oil and fat are extracted by adopting enzyme, oil components are separated from non-oil components by utilizing affinity differences of the non-oil components on oil and water and different proportions of the oil and the water, the production yield is increased, and the extraction rate is increased.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting huperzine A from a Melaleuca tower. Background technique [0002] The Melaleuca Pagoda is a fern of the genus Huperzia in the family Huperzaceae, also known as Huperzia serrata and King of Help. Perennial herb, the whole plant is dark green, 15-40 cm high. root-like. The stem is erect or the lower part is lying down, and the top often has reproductive buds, which fall to the ground to form new seedlings. Leaves papery, elliptic-lanceolate. The sporangia are kidney-shaped, grow horizontally in the leaf axils, with both ends exceeding the leaf edge, light yellow, present on the whole plant. It is mainly distributed in the Northeast, the Yangtze River Basin and Fujian, Guangdong, Guangxi, Yunnan, Guizhou and other provinces. Folks often use the whole herb as medicine, which is poisonous and used for reducing fever, hemostasis, reducin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/22
CPCC07D221/22
Inventor 周晓青钟德品谢冬养陆美珍赵明东
Owner 桂林三宝生物科技有限公司
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