The method of extracting genipin from gardenia

A gardenia, enzymatic hydrolysis technology, applied in the field of extracting genipin from gardenia, can solve the problems of low extraction rate, low product purity, large energy consumption, etc., and achieve improved extraction rate, increased product yield, and transformation high rate effect

Active Publication Date: 2018-11-30
广西优食客食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Geniposide is mainly found in natural plants such as Fructus chinensis and Eucommia ulmoides, and is an ideal raw material for extracting geniposide. In the existing domestic extraction process schemes, there are problems such as low extraction rate, low product purity, large energy consumption, and extensive use Organic solvents, large amount of waste water, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for extracting genipin from gardenia, specifically comprises the following steps:

[0027] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, wait for the water to flow out from the bottom of the pile, and stack them naturally for 1 day;

[0028] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 50°C for 5 minutes, and obtain a paste-like slurry;

[0029] 3) Enzymolysis for the first time: the paste slurry obtained in step 2) is adjusted to pH 4, and 0.05% by weight of the paste slurry is added with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. Part of mannitol was enzymolyzed for 40 minutes to obtain the first enzymolysis solution;

[0030] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 60°C and extract for 120 minutes t...

Embodiment 2

[0037] The method for extracting genipin from gardenia, specifically comprises the following steps:

[0038] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, wait for the water to flow out from the bottom of the pile, and stack them naturally for 1 day;

[0039] 2) Raw material processing: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 60°C for 8 minutes, and obtain a paste-like slurry;

[0040] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / ml of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 50 minutes to obtain the first enzymolysis solution;

[0041] 4) Oil removal: add 3 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obtain...

Embodiment 3

[0048] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, and stack them naturally for 2 days after the water flows out from the bottom of the pile;

[0049] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0050] 3) Enzymolysis for the first time: the paste slurry obtained in step 2) is adjusted to pH 5, and 0.08% by weight of the paste slurry is added with an activity of 30,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. Part of mannitol was enzymatically hydrolyzed for 60 minutes to obtain the first enzymolyzate;

[0051] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and the slag;

[00...

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PUM

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Abstract

The invention discloses a method for extracting genipin from gardenias. The method includes the following steps of pre-wetting stacking, raw material processing, first time of the enzymolysis, de-oiling, second time of enzymolysis, column chromatography, third time of enzymolysis, column chromatography and drying. According to the method, vegetable oil and fat are firstly extracted through enzyme, oil and non-oil components are separated through affinity differences of the non-oil components on oil and water, and different oil and water proportions, and the product yield and the conversation rate are increased.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting genipin from gardenia. Background technique [0002] Jasminoides is the fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a plant of Rubiaceae (Rubiaceae). traditional Chinese medicine. Fructus Sinensis has the effects of purging fire and relieving restlessness, clearing heat and promoting dampness, cooling blood and detoxifying, promoting gallbladder and protecting liver, lowering blood pressure, sterilizing and anti-inflammatory, and externally reducing swelling and relieving pain. Traditional Chinese medicine is mainly used to treat fever, jaundice hepatitis, sprains, high blood pressure, diabetes and so on. There are many chemical components in Fructus japonica, among which there are mainly iridoids such as crocetin, ichietal and geniposide, among which geniposide is the main active ingredient. Geniposide is an ir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 文继承
Owner 广西优食客食品有限公司
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