A method for extracting hypericin from Hypericum perforatum

The technology of Hypericum perforatum and hypericin is applied in the extraction field in the field of biotechnology, which can solve the problems of low product yield and excessive ash content, and achieve the effect of facilitating release and maintaining osmotic pressure.

Inactive Publication Date: 2017-08-11
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing technology used in production is to use methanol as a solvent and sodium hydroxide to balance the pH. The yield of the product produced by this technology is low, the content of hypericin is 0.28-0.33%, and the ash content seriously exceeds the standard
Now with the expansion and competition of the market, 0.3% hypericin can no longer meet the needs of international customers. European and American customers need 0.4% and more than 0.4% hypericin, and the ash content is required to be below 8%, while the traditional process 0.4% hypericin cannot be produced directly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting hypericin from Hypericum perforatum, specifically comprising the steps of:

[0027] 1) Soaking treatment: put Hypericum perforatum into a woven bag, tie it up, put it in a pool, fill up the pool with water, put a heavy object on the pool, and soak for 24 hours;

[0028] 2) Raw material processing: crush the Hypericum perforatum obtained in step 1), add purified water equivalent to twice the weight of the raw material, heat to 60°C for 5 minutes, and obtain a paste-like slurry;

[0029] 3) Enzymolysis: the paste slurry obtained in step 2) is adjusted to pH 5, and 0.05% by weight of the paste slurry is added with an activity of 30,000 active units / gram of solid pectin-fiber compound enzyme and 0.001% by weight of Mannitol was subjected to enzymolysis for 40 minutes to obtain an enzymolysis solution;

[0030] 4) Oil removal: add 2 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 60°C and extract for 120 minute...

Embodiment 2

[0035] A method for extracting hypericin from Hypericum perforatum, specifically comprising the steps of:

[0036] 1) Soaking treatment: put Hypericum perforatum into a woven bag, tie it up, put it in a pool, fill up the pool with water, put a heavy object on the pool, and soak for 18 hours;

[0037] 2) Raw material processing: crush the Hypericum perforatum obtained in step 1), add purified water equivalent to 3 times the weight of the raw material, heat to 50°C for 8 minutes, and obtain a paste-like slurry;

[0038] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 active units / gram of solid pectin-fiber complex enzyme and 0.001% by weight of Mannitol was subjected to enzymolysis for 50 minutes to obtain an enzymolysis solution;

[0039] 4) Oil removal: add 3 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 65°C and extract for 90 minutes to o...

Embodiment 3

[0044] 1) Soaking treatment: put Hypericum perforatum into a woven bag, tie the mouth, put it in a pool, fill up the pool with water, put a heavy object on the pool, and soak for 15 hours;

[0045] 2) Raw material processing: crush the Hypericum perforatum obtained in step 1), add purified water equivalent to twice the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0046] 3) Enzymolysis: the paste slurry obtained in step 2) is adjusted to pH 4, and 0.05% by weight of the paste slurry is added with an activity of 10,000 active units / gram of solid pectin-fiber compound enzyme and 0.001% by weight of Mannitol was subjected to enzymolysis for 60 minutes to obtain an enzymolysis solution;

[0047] 4) Oil removal: add 2 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and slag;

[0048] 5)...

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PUM

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Abstract

The invention discloses a method of extracting hypericin from hypericum perforatum, which includes the following steps: soaking treatment, material processing, enzymolysis, degreasing, alkaline extraction, column chromatography and drying. The method first adopts enzyme to extract vegetable grease, and utilizes the difference between the affinity of non-oil ingredients to oil and the affinity of the non-oil ingredients to water and the different proportions of the oil and the water to separate the oil and the non-oil ingredients, thus increasing product yield and extraction rate. The content of the hypericin obtained by the method is more than 0.5 percent (detected by UV (ultraviolet)), and the ash content is less than 7 percent.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting hypericin from Hypericum perforatum. Background technique [0002] Hypericum perforatum (Hypericum perforatum L), also known as Hypericum perforatum and St. John'swont, is a plant of the genus Hypericum in the family Garcinia, widely distributed in Europe, Asia, northern Africa, Australia, and North and South America. It is one of the herbal medicines recognized and used by humans earlier, and it is distributed in Henan, Hebei, Shandong, Shaanxi, Gansu, Sichuan, Xinjiang and other places in my country. Hypericum perforatum belongs to Garciniaceae and Hypericum. Perennial herbs, 20-60 cm tall, glabrous throughout. The stem is cylindrical, 10-100cm long, multi-branched, with a longitudinal edge on both sides of the stem and branches, and the branchlets are thin and thin, opposite to the leaf axils. Single leaves opposite, sessile, clasp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C50/36C07C46/10
CPCC07C46/10C07C50/36
Inventor 卢照凯谢冬养钟德品周晓青应启实盘秋梅梁小珍
Owner 桂林三宝生物科技有限公司
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