A kind of anaerobic fermentation method of straw
An anaerobic fermentation and straw technology, applied in fermentation, waste fuel, etc., can solve problems such as low methane production and insufficient metabolites, and achieve the effects of increasing production, facilitating absorption and utilization, and increasing methane production
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Embodiment 1
[0025] A method for anaerobic fermentation of stalks, comprising the following steps:
[0026] (1) Crush the straw into 120-mesh powder, add water to soak for 60 minutes, then add potassium sulfite solution accounting for 50% of the powder weight and a concentration of 20%, and cook at 90°C for 2 hours;
[0027] (2) Then add a compound enzyme accounting for 3% of the powder weight and an enzyme activity of 80U / mL to the product obtained in step (1), and perform ultrasonic enzymolysis at 30KHz and 300W for 80min; wherein the compound enzyme includes cellulase, fruit Glue enzyme and proteolytic enzyme, the weight ratio of described cellulase, pectinase and proteolytic enzyme is 2:1:0.8;
[0028] (3) Adding 5% by weight of straw powder and 50% concentration of sodium hydroxide solution to the product obtained in step (2), soaking for 30 minutes;
[0029] (4) Filtrate the product obtained in step (3), separate the straw powder and the solution, then use acetic acid to adjust the ...
Embodiment 2
[0032] A method for anaerobic fermentation of stalks, comprising the following steps:
[0033] (1) Crush the straw into 100-mesh powder, add water to soak for 48 minutes, then add potassium sulfite solution accounting for 34% of the powder weight and a concentration of 24%, and cook at 80°C for 2 hours;
[0034] (2) Add compound enzymes accounting for 2.8% of powder weight and 70U / mL enzyme activity to the product obtained in step (1), and perform ultrasonic enzymolysis at 30KHz and 280W for 40min; wherein the compound enzymes include cellulase, fruit Glue enzyme and proteolytic enzyme, the weight ratio of described cellulase, pectinase and proteolytic enzyme is 1.5:1.2:0.6;
[0035] (3) Add 55% sodium hydroxide solution accounting for 3.8% by weight of the straw powder to the product obtained in step (2) and soak for 30 minutes;
[0036] (4) Filter the product obtained in step (3), separate the straw powder and the solution, then use acetic acid to adjust the pH value of the...
Embodiment 3
[0039] A method for anaerobic fermentation of stalks, comprising the following steps:
[0040] (1) Crush the straw into 120-mesh powder, add water to soak for 60 minutes, then add potassium sulfite solution accounting for 30% of the powder weight and 50% concentration, and cook at 80°C for 1 hour;
[0041] (2) Then add a compound enzyme accounting for 2% of the powder weight and an enzyme activity of 80U / mL to the product obtained in step (1), and perform ultrasonic enzymolysis at 20KHz and 200W for 40min; wherein, the compound enzyme includes cellulase, fruit Glue enzyme and proteolytic enzyme, the weight ratio of described cellulase, pectinase and proteolytic enzyme is 2:2:0.3;
[0042] (3) Add 5% by weight of straw powder and 60% sodium hydroxide solution to the product obtained in step (2), and soak for 20 minutes;
[0043] (4) Filter the product obtained in step (3), separate the straw powder and the solution, then use acetic acid to adjust the pH value of the solution t...
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