A kind of method extracting geniposide from gardenia

A technology of geniposide and gardenia, which is applied in the field of extracting geniposide from gardenia, can solve the problems of low product purity, low extraction rate, large energy consumption, etc., and achieve increased product yield, Improve the extraction rate and facilitate the effect of separation

Active Publication Date: 2018-11-13
广西优食客食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing domestic extraction process schemes, there are problems such as low extraction rate, low product purity, large energy consumption, large use of organic solvents, and large amount of waste water.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting geniposide from Gardenia Fructus, specifically comprising the steps of:

[0025] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, wait for the water to flow out from the bottom of the pile, and stack them naturally for 1 day;

[0026] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 50°C for 5 minutes, and obtain a paste-like slurry;

[0027] 3) Enzymolysis for the first time: the paste slurry obtained in step 2) is adjusted to pH 4, and 0.05% by weight of the paste slurry is added with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. Part of mannitol was enzymolyzed for 40 minutes to obtain the first enzymolysis solution;

[0028] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 60°C and extract for 120 minute...

Embodiment 2

[0033] A method for extracting geniposide from Gardenia Fructus, specifically comprising the steps of:

[0034] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, wait for the water to flow out from the bottom of the pile, and stack them naturally for 1 day;

[0035] 2) Raw material processing: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 60°C for 8 minutes, and obtain a paste-like slurry;

[0036] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / ml of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 50 minutes to obtain the first enzymolysis solution;

[0037] 4) Oil removal: add 3 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obt...

Embodiment 3

[0042] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, and stack them naturally for 2 days after the water flows out from the bottom of the pile;

[0043] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0044] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to be 5, add 0.08% by weight of the paste slurry with an activity of 30,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 60 minutes to obtain the first enzymolysis solution;

[0045] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and the slag;

[0046] 5) Seco...

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PUM

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Abstract

The invention discloses a method for extracting geniposide from gardenia jasminoides ellis. The method comprises steps as follows: prewetting, stacking, raw material processing, first enzymolysis, deoiling, second enzymolysis, column chromatography and drying. According to the method, vegetable oil is extracted with enzyme, oil ingredients and non-oil ingredients are separated according to affinity difference of the non-oil ingredients to oil and water and different specific gravities of oil and water, the yield of products is increased, and the extraction rate is increased.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting geniposide from Gardenia jasminoides. Background technique [0002] Jasminoides is the fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a plant of Rubiaceae (Rubiaceae). traditional Chinese medicine. Fructus Sinensis has the effects of purging fire and relieving restlessness, clearing heat and promoting dampness, cooling blood and detoxifying, promoting gallbladder and protecting liver, lowering blood pressure, sterilizing and anti-inflammatory, and externally reducing swelling and relieving pain. Traditional Chinese medicine is mainly used to treat fever, jaundice hepatitis, sprains, high blood pressure, diabetes and so on. There are many chemical components in Fructus japonica, among which there are mainly iridoids such as crocetin, ichietal and geniposide, among which geniposide is the main active ingredient. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 文继承
Owner 广西优食客食品有限公司
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