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Soybean ACE inhibitory peptide and preparation method and application thereof

A technology for inhibiting peptides and soybeans, applied in the field of soybean ACE inhibitory peptides and their preparation, can solve the problems of poor clarity of soybean peptides, influence of soybean protein on enzymolysis efficiency, low ACE inhibitory activity, etc., so as to facilitate contact probability and reduce production costs. , the effect of reducing the rate of enzymatic hydrolysis

Active Publication Date: 2020-11-13
广东健明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the technical difficulties in the existing method of enzymatically hydrolyzing soybean protein to prepare ACE inhibitory peptides, such as the problems that soybean protein easily absorbs water and swells to affect the enzymatic hydrolysis efficiency, the clarity of soybean peptide is poor, and the ACE inhibitory activity is low. Concentrated soybean protein liquid can expose more enzymatic hydrolysis sites, which is beneficial to the enzymatic hydrolysis

Method used

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  • Soybean ACE inhibitory peptide and preparation method and application thereof
  • Soybean ACE inhibitory peptide and preparation method and application thereof
  • Soybean ACE inhibitory peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A preparation method of soybean ACE inhibitory peptide, comprising the following steps:

[0052] (1) Take 100kg soybean protein isolate and 300kg alkaline solution (pH=10.0) and mix them with high speed stirring (1000r / min), and then obtain soybean protein slurry by colloid grinding.

[0053] (2) Raise the temperature of the soybean protein slurry to 90°C and keep it warm for 1.5 hours, then lower the temperature to 58°C, add 2kg of alkaline protease (Novozym 37071) from Novozymes, keep it at 58°C for 12 hours, and then enzymatically hydrolyze Adjust the pH of the system to 6.0, then add 0.5 kg of neutral protease (Neutrase 1.5MG) from Novozymes to continue the enzymatic hydrolysis for 7 hours; after the enzymatic hydrolysis, raise the temperature to 95°C and keep it warm for 15 minutes to inactivate the enzyme, and then separate it in a centrifuge The supernatant is soybean enzyme hydrolyzate.

[0054] (3) Measure the amount of solid matter in the soybean enzymolysis ...

Embodiment 2

[0059] A preparation method of soybean ACE inhibitory peptide, comprising the following steps:

[0060] (1) Take 100kg of soybean protein isolate and 500kg of alkaline solution (pH=9.5) and mix them with high-speed stirring (800r / min), and then obtain soybean protein slurry through colloidal grinding.

[0061] (2) Raise the temperature of the soybean protein slurry to 95°C and keep it warm for 1.0 hour, then lower the temperature to 62°C, add 1.2kg of Danisco food-grade alkaline protease (FoodPro Alkaline Protease), keep it at 62°C for 14 hours, and then add the enzyme Adjust the pH of the hydrolysis system to 6.5, and then add 0.8kg of Danisco’s neutral protease (Alphalase NP) to continue the enzymolysis for 5 hours; The clear liquid is soybean enzymatic hydrolyzate.

[0062] (3) Measure the amount of solid matter in the soybean enzymatic hydrolysis solution, add 0.6% tea polyphenols and 10.0% diatomaceous earth, then heat up to 70°C and stir for 30 minutes; then pass throug...

Embodiment 3

[0067] A preparation method of soybean ACE inhibitory peptide, comprising the following steps:

[0068] (1) Take 100kg of soybean protein isolate and 400kg of alkaline solution (pH=9.8) and mix them with high-speed stirring (900r / min), and then obtain soybean protein slurry through colloidal grinding.

[0069] (2) Raise the temperature of the soybean protein slurry to 92°C and keep it warm for 1.3 hours, then lower the temperature to 60°C, add 1.6kg of alkaline protease (Novozym 37071) from Novozymes, keep it at 60°C for 13 hours, and then add the enzyme Adjust the pH of the hydrolysis system to 6.3, then add 0.6 kg of neutral protease (Neutrase 1.5MG) from Novozymes to continue the enzymolysis for 6 hours; after the enzymolysis, raise the temperature to 90°C and keep it for 20 minutes to inactivate the enzyme, and then separate it in a centrifuge to obtain The supernatant was soybean enzymatic hydrolyzate.

[0070] (3) Measure the amount of solid matter in the soybean enzymo...

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Abstract

The invention discloses a soybean ACE inhibitory peptide and a preparation method and application thereof. The method comprises the following steps that 1 part by weight of soybean protein isolate and3-5 parts by weight of an alkaline solution are taken, mixed to be uniform and ground into thick liquid, the thick liquid is heated, alkaline protease is added for enzymolysis, the pH value is adjusted, neutral protease is added for hydrolysis, and soybean enzymatic hydrolysate is obtained; and tea polyphenol and diatomite are addded, filtering, and spray drying to is performed obtain the soybeanACE inhibitory peptide. The soybean protein isolate is pretreated by hot alkali so that on one hand, a hydration layer on the surface of the soybean protein can be destroyed, hydrophobic groups in the protein are exposed, the protease cleavage site is increased, and subsequent enzymolysis is facilitated; in addition, part of soybean protein can be hydrolyzed to release polypeptide functional groups with amphipathy so that the pH value of a protein solution is reduced, and the effect of protease is facilitated; more hydrophobic groups are exposed, the protein is degraded, the viscosity of a protein aqueous solution is obviously reduced, and the contact probability of protease and the protein is increased.

Description

technical field [0001] The invention belongs to the field of soybean protein deep processing, and in particular relates to a soybean ACE inhibitory peptide and its preparation method and application. Background technique [0002] Hypertension is a global public health problem. Angiotensin-converting enzyme (ACE) inhibitory peptides are a general term for a class of small molecule polypeptides that can lower blood pressure by inhibiting ACE activity. [0003] Soybean protein isolate obtained from soybeans is rich in nutrition and high in hydrophobic amino acid content, and is an excellent raw material for preparing ACE inhibitory peptides. However, most of the research results of preparing ACE inhibitory peptides from soybean protein isolate are in the laboratory stage, and there are no soybean peptide products with clear antihypertensive effects on the market, mainly because there are still many technical difficulties in the industrial preparation of soybean ACE inhibitory ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23J3/34A23L29/00C12P21/06C07K1/34
CPCA23L33/18A23J3/346A23L29/045C12P21/06C07K1/34A23V2002/00A23V2200/326A23V2250/16A23V2250/2132A23V2250/214
Inventor 徐振秋苏国万赵谋明
Owner 广东健明生物科技有限公司
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