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A method for extracting luteolin from peanut shells

A technology of luteolin and peanut shells, which is applied in the field of extraction in the field of biotechnology, can solve the problems of waste and failure to utilize peanut shells, and achieve the effects of improving extraction rate, facilitating separation, and maintaining osmotic pressure

Inactive Publication Date: 2017-09-22
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Peanut shells are very common in life. Most of the time, they are treated as garbage, some are directly burned, and some are even thrown away directly. It is very wasteful to fail to make good use of peanut shells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for extracting luteolin from peanut shells, specifically comprising the steps of:

[0041] 1) Soaking treatment: Put the peanut shells into a woven bag, tie the mouth, put it in the pool, fill up the pool with water, put a heavy object on the pool, and soak for 15 hours;

[0042] 2) Raw material processing: crush the peanut shell obtained in step 1), add purified water equivalent to 3 times the weight of the raw material, heat to 60°C for 5 minutes, and obtain a paste slurry;

[0043] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 4, add 0.1% by weight of the paste slurry with an activity of 30,000 active units / gram of pectin-cellulose compound enzyme and 0.001 weight portion of manna Carry out enzymolysis with alcohol for 60 minutes to obtain enzymolysis solution;

[0044]4) Oil removal: add 3 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obtain a mixture of...

Embodiment 2

[0049] A method for extracting luteolin from peanut shells, specifically comprising the steps of:

[0050] 1) Soaking treatment: Put the peanut shells into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 20 hours;

[0051] 2) Raw material treatment: crush the peanut shell obtained in step 1), add purified water equivalent to 3 times the weight of the raw material, heat to 50°C for 10 minutes, and obtain a paste slurry;

[0052] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 5, add 0.05% by weight of the paste slurry with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 weight portion of manna Carry out enzymolysis with alcohol for 40 minutes to obtain enzymolysis solution;

[0053] 4) Oil removal: add 3 times the weight of pure water to the enzymolysis solution obtained in step 3), heat to 60°C and extract for 60 minutes to obtain a mixture o...

Embodiment 3

[0058] A method for extracting luteolin from peanut shells, specifically comprising the steps of:

[0059] 1) Soaking treatment: Put the peanut shells into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 24 hours;

[0060] 2) Raw material treatment: crush the peanut shells obtained in step 1), add purified water equivalent to twice the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0061] 3) Enzymolysis: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 weight portion of manna Carry out enzymolysis with alcohol for 50 minutes to obtain enzymolysis solution;

[0062] 4) Oil removal: Add 2 times the weight of purified water to the enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a...

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PUM

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Abstract

The invention discloses a method for extracting luteolin from peanut shells. The method comprises the following steps of steeping treatment, raw material treatment, enzymolysis, deoiling, alkaline extraction, extraction and recrystallization. According to the method, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting luteolin from peanut shells. Background technique [0002] Peanut shell is the shell of peanut. The peanut fruit is a pod, and the shapes are cocoon-shaped, bead-shaped and hockey stick-shaped. The color of the shell is mostly yellow-white, but also yellow-brown, brown or yellow, which is related to the variety of peanuts and soil quality. [0003] Peanut shells are very common in life. Most of the time, they are treated as garbage, some are burned directly, and some are even thrown away directly. The peanut shells cannot be used well, which is very wasteful. Plants are full of treasures, and after scientific treatment, they will bring more benefits to people. Peanut shells contain nearly 60% crude fiber, ranking first in roughage. Dry matter accounts for 90.3% of the nutrients in peanut shells, including 4.8-7.2% crude protein, 1-1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 卢照凯陆美珍谢冬养赵明东钟德品梁小珍周晓青
Owner 桂林三宝生物科技有限公司
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