Method for preparing sesame oil by solid state fermentation aqueous enzymatic method
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A solid-state fermentation, water enzymatic technology, applied in the direction of fermentation, fat oil/fat production, fat production, etc., to achieve the effects of good quality, high nutritional value and high oil extraction rate
Inactive Publication Date: 2013-11-13
NORTHEAST AGRICULTURAL UNIVERSITY
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At present, there is no report on the preparation of sesame oil by solid-state fermentation water enzymatic method
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Embodiment 1
[0022] Embodiment 1: Screening test of optimal parameters of sesame solid-state fermentation process
[0023] 1 Materials and methods
[0024] 1.1 Materials and reagents
[0025] Sesame commercially available Bacillus subtilis Key Laboratory of Northeast Agricultural University Alcalase alkaline endoprotease novo (1.2 x 10 5 U / mL)
[0026] 1.2 Main instruments and equipment
[0027] pHS-25 acidity meter Shanghai Weiye Instrument Factory electronic analytical balance Merlot-Toledo Instruments (Shanghai) Co., Ltd. F2102 plant sample pulverizer Tianjin Test Instrument Co., Ltd. Electric constant temperature water bath Yuyao Dongfang Electrical Instrument Factory Precision electric mixer Ronghua Instrument Manufacturing Co., Ltd., Jintan City, Jiangsu Province Autoclave Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory Constant temperature incubator Beijing Yongguangming Medical Instr...
Embodiment 2
[0053] Example 2: Screening test for optimal parameters of sesame fermentation product enzymatic hydrolysis process
[0054] 1 Materials and methods
[0055] 1.1 Materials and reagents
[0056] Sesame commercially available Bacillus subtilis Key Laboratory of Northeast Agricultural University Alcalase alkaline endoprotease novo (1.2 x 10 5 U / mL)
[0057] 1.2 Main instruments and equipment
[0058] pHS-25 acidity meter Shanghai Weiye Instrument Factory electronic analytical balance Merlot-Toledo Instruments (Shanghai) Co., Ltd. F2102 plant sample pulverizer Tianjin Test Instrument Co., Ltd. Electric constant temperature water bath Yuyao Dongfang Electrical Instrument Factory Precision electric mixer Ronghua Instrument Manufacturing Co., Ltd., Jintan City, Jiangsu Province Autoclave Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory Constant temperature incubator Beijing Yongguangming ...
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Abstract
The invention discloses a method for preparing sesame oil by a solid state fermentation aqueous enzymatic method and belongs to the field of plant oil extraction and processing. The method comprises the following steps of 1, adjusting moisture of hulled sesame, carrying out autoclaved sterilization, cooling the sterile sesame, putting the cooled sesame into a solid state fermentation tank, and carrying out solid state fermentation to obtain fermentation products, and 2, crushing the fermentation products obtained by the step 1, mixing the crushed fermentation products and water, adding protease into the mixture, carrying out enzymatic hydrolysis, carrying out alkaline extraction, carrying out centrifugalization to obtain free oil, an emulsion, a hydrolysate and residues, carrying out demulsification of the emulsion and the hydrolysate, and merging the free oil, and the emulsion and the hydrolysate subjected by demulsification to obtain the sesame oil. The method realizes sesame oil preparation by combination of solid state fermentation and the biological enzyme, adopts simple equipment, is safe in operation, has a high oil yield, can produce the sesame oil having good quality and a high nutrition value, can produce a high-activity sesame protein and a sesame peptide under the action of the biological compound enzyme, and can reduce protein resource waste.
Description
technical field [0001] The invention belongs to vegetable oil extraction and processing technology, and mainly relates to a method for preparing sesame oil by solid-state fermentation and water enzymatic method. Background technique [0002] Microbial solid-state fermentation technology uses natural substrates as carbon sources and energy sources, or uses inert substrates as solid supports. The water content of the system is low. When dealing with oil crops, the compound action of various enzymes produced by microbial fermentation can destroy the cell wall of the material. It can also destroy the composite structure of lipoprotein bodies, which is beneficial to the release of oil. It has the characteristics of simple operation, large processing capacity, low cost and thorough treatment. It can achieve the purpose of purifying the environment and recycling beneficial products. attention. [0003] Water enzymatic oil extraction is a novel oil extraction technology, which is s...
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