A method for extracting geniposide from gardenia

A technology of geniposide and gardenia, which is applied in the field of extracting geniposide from gardenia, can solve the problems of high energy consumption, low product purity, and low extraction rate, so as to increase product yield and improve extraction rate , the effect of releasing

Inactive Publication Date: 2018-01-30
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Geniposide is mainly found in natural plants such as Fructus chinensis and Eucommia ulmoides, and is an ideal raw material for extracting geniposide. In the existing domestic extraction process schemes, there are problems such as low extraction rate, low product purity, large energy consumption, and extensive use Organic solvents, large amount of waste water, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting geniposide from gardenia, specifically comprising the steps of:

[0025] 1) Soaking treatment: Put the gardenia into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 15 hours;

[0026] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 50°C for 5 minutes, and obtain a paste-like slurry;

[0027] 3) Enzymolysis for the first time: the paste slurry obtained in step 2) is adjusted to pH 4, and 0.05% by weight of the paste slurry is added with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. Part of mannitol was enzymolyzed for 40 minutes to obtain the first enzymolysis solution;

[0028] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 60°C and extract for 120 minutes to obt...

Embodiment 2

[0033] A method for extracting geniposide from gardenia, specifically comprising the steps of:

[0034] 1) Soaking treatment: Put the gardenia into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 24 hours;

[0035] 2) Raw material processing: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 60°C for 8 minutes, and obtain a paste-like slurry;

[0036] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / ml of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 50 minutes to obtain the first enzymolysis solution;

[0037] 4) Oil removal: add 3 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obtain a mi...

Embodiment 3

[0042] 1) Soaking treatment: Put the gardenia into a woven bag, tie the mouth, put it in the pool, fill the pool with water, put a heavy object on the pool, and soak for 18 hours;

[0043] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0044] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to be 5, add 0.08% by weight of the paste slurry with an activity of 30,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 60 minutes to obtain the first enzymolysis solution;

[0045] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and the slag;

[0046] ...

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PUM

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Abstract

The invention discloses a method for extracting geniposide from gardenia jasminoides, which comprises the following steps: soaking treatment, raw material treatment, first enzymolysis, oil removal, second enzymolysis, column chromatography and drying. The invention first uses enzymes to extract vegetable oils, and utilizes the affinity difference of non-oil components to oil and water and the specific gravity of oil and water to separate oil and non-oil components, thereby increasing product yield and improving extraction rate.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting geniposide from Gardenia jasminoides. Background technique [0002] Jasminoides is the fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a plant of Rubiaceae (Rubiaceae). traditional Chinese medicine. Fructus Sinensis has the effects of purging fire and relieving restlessness, clearing heat and promoting dampness, cooling blood and detoxifying, promoting gallbladder and protecting liver, lowering blood pressure, sterilizing and anti-inflammatory, and externally reducing swelling and relieving pain. Traditional Chinese medicine is mainly used to treat fever, jaundice hepatitis, sprains, high blood pressure, diabetes and so on. There are many chemical components in Fructus japonica, among which there are mainly iridoids such as crocetin, ichietal and geniposide, among which geniposide is the main active ingredient. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 钟德品谢冬养陆美珍赵明东应启实卢桦香盘秋梅
Owner 桂林三宝生物科技有限公司
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