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Preparation method and application of trans-crocetin

A technology of trans-crocus acid and crocus acid, applied in the field of medicine, can solve problems such as unfavorable environmental protection, limitation, unsuitability for large-scale production, etc., and achieve the effects of cost reduction and simple method

Active Publication Date: 2010-08-25
施冬云 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses ethanol extraction or ethanol precipitation in the extraction, and the method of ultrasonically enhanced extraction is limited by equipment and is not suitable for large-scale production, and it is first extracted and then concentrated or the eluent is concentrated after the macroporous resin The process of acid-base hydrolysis in the future is cumbersome, there are multiple concentration processes, and a large amount of energy is consumed, which is not conducive to environmental protection

Method used

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  • Preparation method and application of trans-crocetin
  • Preparation method and application of trans-crocetin
  • Preparation method and application of trans-crocetin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Gardenia jasminodes Ellis (Gardenia jasminodes Ellis) fruit 10kg, pulverized through a 40 mesh sieve, extracted twice with 60L and 40L of 30% dilute ethanol solution (adjusted pH = 12 with sodium hydroxide) twice, the first time for 3 hours, the second time The second time is 1.5 hours, and the extract is obtained by filtration;

[0036] (2) The extract was adjusted to pH=8 with hydrochloric acid, concentrated under reduced pressure to about 5 L;

[0037] (3) filter, get the filtrate to obtain concentrated clear liquid;

[0038](4) Slowly add hydrochloric acid solution dropwise to the concentrated supernatant, adjust pH = 3, let it stand for 4 hours, filter to obtain a brick red precipitate, wash the precipitate twice with a dilute ethanol solution of hydrochloric acid (ethanol concentration: 20%) with pH = 5 , washed once with pure water, and dried to obtain the crude product of crocetin, wherein the content of trans-crocetin is 81.4%.

Embodiment 2

[0040] (1) 10 kg of Gardenia jasmine fruit, crushed through a 5-mesh sieve, extracted by percolation with 80 L of 60% dilute methanol solution (adjusting pH 10 with potassium hydroxide), and centrifuged to obtain the extract;

[0041] (2) The extract was adjusted to pH=7 with acetic acid, concentrated under reduced pressure to 2L;

[0042] (3) filter, get the filtrate to obtain concentrated clear liquid;

[0043] (4) Slowly add acetic acid solution dropwise in the concentrated clear liquid, adjust the pH to 5, let it stand for 0.5 hour, filter to obtain a brick red precipitate, wash the precipitate twice with dilute acetate alcohol (40% ethanol concentration) with pH=4, and pure Wash twice with water and dry to obtain crude crocetin, in which the content of trans-crocetin is 86.2%.

Embodiment 3

[0045] (1) 1 kg of pistil of saffron, crushed through a 100-mesh sieve, soaked and extracted three times with 7L, 5L, and 4L water respectively (adjusting pH=14 with potassium hydroxide), filtered, and combined the filtrates to obtain the extract;

[0046] (2) The extract was adjusted to pH=9 with hydrochloric acid, concentrated under reduced pressure to 1L;

[0047] (3) filter, get filtrate, obtain concentrated supernatant;

[0048] (4) Slowly add hydrochloric acid solution dropwise to the concentrated clear liquid, adjust the pH to 2, let stand for 12 hours, filter to obtain a brick-red precipitate, wash the precipitate once with pH = 6 dilute alcohol (30% ethanol concentration), and pure water Wash once and dry to obtain crude crocetin, wherein the content of trans-crocetin is 80.5%.

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Abstract

The invention relates to the medicine field, and discloses a preparation method of crocetin. Extraction is carried out by adopting diluted alkali, extraction condensation and hydrolysis are combined together, and crocin is hydrolyzed and converted into crocetin during extraction and concentration. The preparation method comprises the steps of: crushing raw materials, carrying out conversion extraction by using a diluted alcoholic solution with pH of 10-14, regulating the pH to be 7-9 by using an extracting solution, condensing by decompression, filtering or centrifuging to remove sediment impurities, regulating the pH to be 2-5, filtering or centrifuging to collect red sediments and obtain a crocetin crude product with crocetin content higher than 50 percent, and recrystallizing and purifying the crocetin crude product to obtain the high-purity trans-crocetin with the crocetin content higher than 95 percent. The trans-crocetin can be used for preparing a medicament for treating fattyliver and has wide application prospect in prevention and control of tumors and other diseases.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for preparing crocetin, which is extracted and transformed from natural plants to obtain trans-crocetin, which has remarkable efficacy in treating fatty liver. Background technique [0002] Crocetin is the aglycon of crocetin, and it is one of the active ingredients isolated and extracted from saffron, a plant of the genus Crocus in the family Iridaceae. It has a polyunsaturated conjugated enoic acid structure and is a carotenoid substance. The content of crocetin in natural plants such as gardenia and saffron is relatively high; in addition to crocetin, natural plants such as gardenia and saffron also contain a large amount of crocetin, namely crocetin And sugar complexes, such as crocin 1, 2, 3, 4. Under certain conditions, the glycosidic bond of crocin is hydrolyzed, and the sugar ligand is removed to generate crocetin. [0003] Studies have found that crocetin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/13C07C51/42A61K31/202A61P1/16
Inventor 施冬云刘珊林谢飞舟
Owner 施冬云
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