Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition, Crocus sativus pigment active site, and uses of composition and active site

The technology of saffron pigment and active parts is applied in the field of compositions, active parts of saffron pigments and their uses, and can solve the problems of high effective dose, unknown composition and content, low purity of saffron-enriched parts, and the like, and achieves a simple preparation process. Effect

Active Publication Date: 2016-09-14
JINAN UNIVERSITY
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent document focuses on the 50% ethanol part, and the process of using a macroporous resin column and a Sephadex column is relatively complicated. In addition, the composition and content of the saffron-enriched part in this patent document are unknown. And the effective dosage in the embodiment is also higher (100~400mg), and this probably is because the purity of the enriched crocin part wherein is relatively low causes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition, Crocus sativus pigment active site, and uses of composition and active site
  • Composition, Crocus sativus pigment active site, and uses of composition and active site
  • Composition, Crocus sativus pigment active site, and uses of composition and active site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1: the preparation method of gardenia saffron pigment active part

[0097] Take 40.0 kg of dried and mature fruits of Gardenia jasminoides, after being crushed properly, heat and reflux extract with 4 times the amount of 60% ethanol for 3 times, each time for 2 hours. The extracts were combined, and the solvent was evaporated under reduced pressure to obtain 6.2 kg of the total gardenia extract; the extract was dissolved in an appropriate amount of water, centrifuged, and subjected to macroporous resin open column chromatography (20.0 × 90 cm), followed by 4 times the volume of the column bed Water, 30%, 50%, 70%, and 95% ethanol were used for gradient elution, and each part of the eluate was collected, and the solvent was recovered under reduced pressure, respectively, to obtain about 4.5kg of water elution and 30% ethanol elution combination, 50 The fraction eluted with % ethanol was 710.0 g, the fraction eluted with 70% ethanol was 150.0 g, the fraction el...

Embodiment 2

[0098] Example 2: Isolation and identification of main components in active parts of gardenia saffron pigments

[0099] The UPLC characteristic collection of spectra of the gardenia saffron pigments active site prepared in embodiment 1 is as follows figure 1 shown. Under the guidance of the characteristic map, through ODS column chromatography, RP-HPLC preparation liquid phase separation means, using UV, MS, NMR and other analysis and identification methods, identified crocetin bis-β-D-gentiobioside and crocetin-β -D-glucopyranosyl-β-D-gentiobioside, crocetin bis-β-D-glucopyranoside, 13Z-crocetin bis-β-D-gentiobioside, neocrocin B, crocetin Mono-β-D-gentiobioside, 13Z-crocetin-8-O-β-D-gentiobioside, 13Z-crocetin-8′-O-β-D-gentiobioside, crocetin Structures of these nine compounds of mono-β-D-glucopyranoside.

[0100] Under the same chromatographic conditions as the UPLC characteristic map of the active part of gardenia saffron pigments, the isolated compounds were identified...

Embodiment 3

[0150] Example 3: GJ-4, the active part of gardenia saffron pigments, improves learning and memory impairment in mice induced by scopolamine (platform test)

[0151] 3.1 Principle of platform jumping experiment

[0152] The platform experiment device is a rectangular reflection box with a size of 10cm×10cm×60cm. It is divided into 5 rooms with black plastic boards. The bottom surface is covered with copper grids with a distance of 0.5cm. It can be energized, and the voltage intensity is controlled by a transformer. A wooden platform with a height and a diameter of 4.5 cm was placed, and a 36V alternating current was applied during the experiment. After the mice received the electric shock, their normal reaction was to jump back to the safe platform to avoid the noxious stimulus. On the first day without electricity, the mice were put into the reflex box to move freely for 5 minutes to get familiar with the environment; after 24 hours, the copper grid power supply (36V alternat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a composition, and also relates to a Crocus sativus pigment active site extracted from Fructus Gardeniae. The composition mainly comprises trans-crocetin di(beta-D-gentiobiosyl)ester, trans-crocetin-beta-D-gentiobiosyl-beta-D-glucosyl ester, trans-crocetin di(beta-D-glucosyl)ester, 13-cis-crocetin di(beta-D-gentiobiosyl)ester, neocrocin B, trans-crocetin mono(beta-D-gentiobiosyl)ester, 13-cis-crocetin-8-O-beta-D-gentiobiosyl ester, 13-cis-crocetin-8'-O-beta-D-gentiobiosyl ester and trans-crocetin mono(beta-D-glucosyl)ester. Pharmacological experiment results show that the Crocus sativus pigment active site can effectively improve mouse learning memory injuries induced by scopolamine and amyloid beta protein.

Description

technical field [0001] The present invention relates to a composition, and also to a traditional Chinese medicine extract, in particular, to a saffron pigment active part extracted from Gardenia jasmine and its use in the prevention and treatment of diseases such as Alzheimer's disease , more specifically, relates to the application of an active part of gardenia saffron pigments and its contained active ingredients in the preparation of medicines or health care products for preventing and treating Alzheimer's disease and other senile dementia-related diseases. Background technique [0002] Saffron pigment is a kind of water-soluble carotenoid with unique structure, including saffron acid and its sugar esters combined with different sugar groups. It is a common pigment component in saffron and gardenia. Due to its good water solubility, saffron pigment is widely used as a coloring agent in wine, dishes and cakes. A number of studies have shown that the crude extract of saffr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61P25/28A61K36/88C07H13/06A23L33/105
CPCA61K31/7024A61K36/88A23V2002/00A23V2200/322A23V2250/21A23L33/105C07H13/04A61K36/744A61K2236/00A61P25/28C07H13/06A61K2236/15A61K2236/331A61K2236/333A61K2236/51A61K2236/53
Inventor 姚新生张丹于洋鲍秀琦倪阳臧彩霞高昊郑远鹏
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com