Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for integral preparation of products such as crocetin, genipin, gardenia blue and the like

A technology of saffron acid and genipin, applied in chemical instruments and methods, separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of difficulty in large-scale cultivation, high price and limited resources, etc. The effect of low equipment requirements, simple operation and short production cycle

Active Publication Date: 2014-04-02
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, saffron is only used as medicine on the stigma, and the yield is extremely low. Because of its extremely limited resources, it is difficult to cultivate it in a large area in China, which makes it expensive and known as "plant gold" and has extremely high medical value.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for integral preparation of products such as crocetin, genipin, gardenia blue and the like
  • Method for integral preparation of products such as crocetin, genipin, gardenia blue and the like
  • Method for integral preparation of products such as crocetin, genipin, gardenia blue and the like

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Preparation of crocetin.

[0036] (1) 200g of gardenia fruit was crushed and extracted twice with 1000ml of methanol. After removing the medicinal residues by filtration, the two extracts were combined and concentrated to 200ml.

[0037] (2) Add NaOH to the concentrated solution to adjust the pH to 10.0, and place it in an ultrasonic generator for reaction. The temperature of the reaction process was controlled at 28° C., the ultrasonic frequency was 40 kHz, the power was 100 W, and the reaction time was 10 minutes.

[0038] (3) After the reaction, the yellow-brown precipitate produced in the solution was collected by suction filtration.

[0039] (4) Take the yellow-brown precipitate and add 100 ml of alkaline water with a pH of 10. After it is completely dissolved, add 2 mol / L HCl aqueous solution to adjust the pH to 3. At this time, a large amount of brick-red precipitates are produced in the solution. The resulting brick-red precipitate was ...

Embodiment 2

[0044] Embodiment 2: Preparation of Genipin.

[0045] (1) Take 200 ml of the methanol filtrate obtained from the alkali hydrolysis reaction, evaporate the methanol to dryness, reconstitute with 200 ml of pure water, and add ethyl acetate at a volume ratio of 1:1 to construct a two-phase system.

[0046] (2) Add medium-temperature cellulase with an enzyme activity of 20wU (amount of 5%) into the two-phase system, and place it in an ultrasonic generator for reaction. The temperature during the reaction is controlled at 50°C, and the pH is adjusted to 4.5 with acetic acid. The frequency is 40kHz, the power is 100W, and the response time is 8 hours.

[0047] (3) The organic phase layer after the reaction was taken, washed 3 times with an equal volume of pure water, concentrated and evaporated to dryness to obtain 6.3 g of crude genipin with a purity of more than 75%.

[0048] (4) Take 2.0 g of crude genipin, dissolve it in a methanol-water system at 50°C, put it in a re...

Embodiment 3

[0052] Embodiment 3: the preparation of gardenia blue pigment.

[0053] (1) Take 100.0 g of crude genipin (content 75%) and dissolve it in 10 L of distilled water.

[0054] (2) Add 200.0g of sodium glutamate with a purity of more than 99% to the aqueous solution, and place it in an ultrasonic generator for reaction. The temperature of the reaction process is controlled at 50°C, the ultrasonic frequency is 40kHz, the power is 100W, and the reaction time is 24 hours. After the reaction, the obtained solution is an aqueous solution of gardenia blue crude product, and the color value is E590>60.

[0055] (3) The crude gardenia blue aqueous solution after the reaction is intercepted and concentrated by using a hollow cellulose membrane with a cut-off pore size of 3000-5000 until the solid content in the retained solution exceeds 15%.

[0056] (4) The retentate was spray-dried to obtain 164.7 g of gardenia blue powder with color value E590>110. (Determination of color v...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the fields of biotechnology and comprehensive utilization of a natural product, and particularly relates to a method for integral preparation of a plurality of products with high additional value, such as crocetin, genipin, gardenia blue and the like by taking jasmine as a raw material through crushing, alcohol extraction and concentration without complicated separation means of resin, silica gel and the like by adopting simple chemical modification and biological catalysis technologies through a set of production technology. The purities of the prepared crocetin and genipin can be up to over 95%, and the color value E590 of the gardenia blue is greater than 110.

Description

technical field [0001] The invention relates to the fields of biotechnology and comprehensive utilization of natural products, in particular to a method for integrated preparation of various high-tech value-added products such as crocetin, genipin and gardenia blue by using gardenia as raw materials. The method requires low equipment, is easy to operate, and does not need complex separation methods such as resin and silica gel, and can simultaneously produce a variety of deep-processed products in a set of production processes. Background technique [0002] Gardenia jasminoides Ellis, also known as mountain gardenia, yellow gardenia, red gardenia, white cicada, etc., is an evergreen shrub belonging to Rubiaceae Gardenia. Gardenia is bitter and cold in nature and non-toxic. It is often used to clear away heat and relieve fire, detoxify Yuzhi, and clear blood in the stomach. Modern pharmacological studies have shown that gardenia has antipyretic and sedative effects, protects...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/06C12P17/12C07C57/13C07C51/42C09B61/00
Inventor 赵伯涛朱羽尧钱骅黄晓德陈斌
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com