Soothing anti-inflammatory composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of skin care product raw materials, and particularly discloses a soothing anti-inflammatory composition as well as a preparation method and application thereof. The preparation method of the soothing anti-inflammatory composition comprises the following steps: step 1) mixing saffron crocus, radix sophorae flavescentis, houttuynia cordata, moringa seeds and astragalus membranaceus to obtain a composite raw material; the invention discloses a soothing and anti-inflammatory composition and a preparation method thereof.The soothing and anti-inflammatory composition and the preparation method thereof.The soothing and anti-inflammatory composition has the advantages that soothing, anti-inflammatory and skin barrier repairing effects are further improved.

Description

Technical Field

[0001] This invention relates to the field of skincare ingredients, and in particular to a soothing and anti-inflammatory composition, its preparation method, and its application. Background Technology

[0002] With increasing environmental pollution, rising life pressures, and excessive use of cosmetics, the number of people with sensitive skin is increasing year by year, and problems such as stinging, redness, and itching caused by damage to the skin barrier are becoming increasingly prominent.

[0003] Existing skincare products for sensitive skin mainly achieve their soothing and anti-inflammatory effects by adding ingredients with calming and anti-inflammatory properties. Because sensitive skin is more fragile, natural plant extracts are usually added as soothing and anti-inflammatory ingredients. Although existing products have certain soothing and anti-inflammatory effects, there is a lack of research on the combination of plant extracts, resulting in relatively limited soothing and anti-inflammatory effects. The skin conditioning effect is slow to take effect, and there is a lack of the ability to repair the skin barrier, making it difficult for the skin to be completely improved. There is also a greater dependence on soothing products. Therefore, there is still room for improvement. Summary of the Invention

[0004] To further enhance the soothing, anti-inflammatory, and skin barrier repair effects, this application provides a soothing and anti-inflammatory composition, its preparation method, and its application.

[0005] In a first aspect, this application provides a method for preparing a soothing and anti-inflammatory composition, employing the following technical solution: A method for preparing a soothing and anti-inflammatory composition includes the following steps: Step 1) Mix saffron, sophora flavescens root, houttuynia cordata, moringa seeds and astragalus to obtain compound raw materials; Step 2), extract the composite raw materials in an ethanol-water solution, filter, and collect the filtrate; Step 3) Concentrate and dry the filtrate to obtain a soothing and anti-inflammatory composition.

[0006] By adopting the above technical solution, and by using a mixture of saffron, sophora flavescens root, houttuynia cordata, moringa seed, and astragalus extract, the resulting composition achieves multi-target synergy through the interaction of various active ingredients. This results in effective soothing and anti-inflammatory effects from multiple dimensions, making the soothing and anti-inflammatory effects very significant and fast-acting. At the same time, it can effectively activate skin activity, promote the repair of the skin barrier, and quickly transform sensitive skin into normal skin. It can thoroughly improve sensitive skin and well meet the needs of consumers.

[0007] Preferably, in step 1), the mass ratio of saffron, sophora flavescens root, houttuynia cordata, moringa seeds, and astragalus is 3-4:2-3:1-2:1-2:1-2.

[0008] By adopting the above technical solution and specifically selecting the mass ratio of saffron, sophora flavescens root, houttuynia cordata, moringa seed, and astragalus, the active ingredients are formulated in a better proportion, resulting in a better synergistic effect and more significant soothing, anti-inflammatory, and skin barrier repair effects.

[0009] Preferably, in step 1), saffron, sophora flavescens root, houttuynia cordata, moringa seeds, and astragalus are mixed and then crushed to obtain a composite raw material of 80-100 mesh.

[0010] By adopting the above technical solution and crushing, extraction efficiency can be improved, process time waste can be reduced, and higher economic value can be achieved.

[0011] Preferably, in step 2), the ethanol aqueous solution contains 70-75% ethanol by mass.

[0012] By adopting the above technical solution and specifically selecting the mass content of ethanol, the extraction efficiency is high, the extraction effect is good, and the quality of the composition is better.

[0013] Preferably, in step 2), the composite raw material is extracted in an ethanol-water solution for 1-2 hours.

[0014] By adopting the above technical solution, extraction can be completed in 1-2 hours, achieving good extraction results while reducing time waste and thus having higher economic value.

[0015] Preferably, in step 2), the composite raw materials are extracted in an ethanol-water solution at a constant temperature of 60-70℃ for 1-2 hours.

[0016] By adopting the above technical solution and extracting at a constant temperature of 60-70℃, the extraction efficiency is higher, the quality of the composition is better, and the efficacy is better.

[0017] Preferably, in step 2), the mass ratio of the composite raw material to the ethanol aqueous solution is 1:10-20.

[0018] By adopting the above technical solution and specifically selecting the mass ratio of the composite raw materials to the ethanol aqueous solution, it is beneficial to improve the extraction efficiency and reduce the energy consumption required for subsequent concentration and drying, thus having higher economic value.

[0019] Secondly, this application provides a soothing and anti-inflammatory composition, employing the following technical solution: A soothing and anti-inflammatory composition is prepared by the above-described method for preparing a soothing and anti-inflammatory composition.

[0020] By adopting the above technical solution, the soothing and anti-inflammatory composition has significant soothing and anti-inflammatory effects, which can quickly relieve the discomfort caused by skin allergies, while also promoting the repair of the skin barrier, effectively improving sensitive skin and restoring the skin to health.

[0021] Thirdly, this application provides an application of a soothing and anti-inflammatory composition, employing the following technical solution: Application of a soothing and anti-inflammatory composition, wherein the soothing and anti-inflammatory composition is added to a skin care product formulation for the preparation of a skin care product with soothing and anti-inflammatory effects.

[0022] By adopting the above technical solution, skin care products with added soothing and anti-inflammatory compositions have excellent soothing, anti-inflammatory and skin barrier repair effects, and can effectively repair the skin and restore it to health.

[0023] In summary, this application has the following beneficial effects: 1. Because this application uses a mixture of saffron, sophora flavescens root, houttuynia cordata, moringa seed, and astragalus to extract the composition, the combination of various active ingredients can achieve multi-target synergy, effectively soothing and reducing inflammation from multiple dimensions. This results in significant and rapid soothing and anti-inflammatory effects. At the same time, it can effectively activate skin activity, promote the repair of the skin barrier, and quickly transform sensitive skin into normal skin. It can thoroughly improve sensitive skin and well meet the needs of consumers.

[0024] 2. In this application, the preferred method is to select the specific mass ratio of saffron, sophora flavescens root, houttuynia cordata, moringa seed, and astragalus to better formulate the active ingredients in a special ratio, thereby making the synergistic effect of the active ingredients better and the soothing, anti-inflammatory and skin barrier repair effects more significant. Detailed Implementation

[0025] The present application will be further described in detail below with reference to the embodiments.

[0026] Example 1 A soothing and anti-inflammatory composition, prepared by means of the following steps: Step 1): Mix 3kg saffron, 2kg sophora flavescens root, 1kg houttuynia cordata, 1kg moringa seeds and 1kg astragalus membranaceus, crush and sieve to obtain 80-mesh composite raw material.

[0027] Step 2): Add 80 kg of 70% ethanol aqueous solution to the stirred tank, then add the composite raw material to the ethanol aqueous solution, heat and keep the temperature constant at 60℃, rotate at 30 r / min, stir and extract for 1 h, filter through filter cloth and take the filtrate.

[0028] Step 3) The filtrate is concentrated by rotary evaporation at a pressure of 0.01 MPa and a temperature of 60°C to obtain a paste. The paste is then spray-dried to obtain a soothing and anti-inflammatory composition.

[0029] The saffron was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0030] The Sophora flavescens root was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0031] The houttuynia cordata was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0032] Moringa seeds were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., dried product.

[0033] Astragalus was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0034] Example 2 A soothing and anti-inflammatory composition, prepared by means of the following steps: Step 1): Mix 3.5kg saffron, 2.5kg sophora flavescens root, 1.5kg houttuynia cordata, 1.5kg moringa seeds and 1.5kg astragalus membranaceus, crush and sieve to obtain 80-mesh composite raw material.

[0035] Step 2): Add 157.5 kg of 70% ethanol aqueous solution to the stirred tank, then add the composite raw material to the ethanol aqueous solution, heat and keep the temperature constant at 60℃, rotate at 30 r / min, stir and extract for 1 h, filter through filter cloth and take the filtrate.

[0036] Step 3) The filtrate is concentrated by rotary evaporation at a pressure of 0.01 MPa and a temperature of 60°C to obtain a paste. The paste is then spray-dried to obtain a soothing and anti-inflammatory composition.

[0037] The saffron was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0038] The Sophora flavescens root was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0039] The houttuynia cordata was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0040] Moringa seeds were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., dried product.

[0041] Astragalus was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0042] Example 3 A soothing and anti-inflammatory composition, prepared by means of the following steps: Step 1): Mix 4kg saffron, 3kg sophora flavescens root, 2kg houttuynia cordata, 2kg moringa seeds and 2kg astragalus membranaceus, crush and sieve to obtain a 100-mesh composite raw material.

[0043] Step 2): Add 260 kg of 75% ethanol aqueous solution to the stirred tank, then add the composite raw material to the ethanol aqueous solution, heat and keep the temperature at 70℃, rotate at 30 r / min, stir and extract for 2 h, filter through filter cloth and take the filtrate.

[0044] Step 3) The filtrate is concentrated by rotary evaporation at a pressure of 0.01 MPa and a temperature of 60°C to obtain a paste. The paste is then spray-dried to obtain a soothing and anti-inflammatory composition.

[0045] The saffron was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0046] The Sophora flavescens root was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0047] The houttuynia cordata was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0048] Moringa seeds were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., dried product.

[0049] Astragalus was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd., in dried form.

[0050] Comparative Example 1 A soothing and anti-inflammatory composition, differing from Example 2 only in that: Replace saffron with an equal amount of Centella asiatica.

[0051] Centella asiatica is sourced from commercially available dried products.

[0052] Comparative Example 2 A soothing and anti-inflammatory composition, differing from Example 2 only in that: Gentian root was used to replace Sophora flavescens root in an equal amount.

[0053] Gentian root is sourced from commercially available dried products.

[0054] Comparative Example 3 A soothing and anti-inflammatory composition, differing from Example 2 only in that: Replace houttuynia cordata with an equal amount of purslane.

[0055] Purslane is sourced from commercially available dried products.

[0056] Comparative Example 4 A soothing and anti-inflammatory composition, differing from Example 2 only in that: Moringa seeds were replaced with an equal amount of chamomile.

[0057] Chamomile is sourced from commercially available dried products.

[0058] Comparative Example 5 A soothing and anti-inflammatory composition, differing from Example 2 only in that: Glycyrrhiza uralensis was used to replace Astragalus membranaceus in equal amounts.

[0059] Licorice root is sourced from commercially available dried products.

[0060] Comparative Example 6 A soothing and anti-inflammatory composition, differing from Example 2 only in that: Equal amounts of Centella asiatica were used to replace saffron, equal amounts of Gentiana scabra root were used to replace Sophora flavescens root, equal amounts of Portulaca oleracea root were used to replace Houttuynia cordata root, equal amounts of Chamomile rhizome were used to replace Moringa seeds, and equal amounts of Glycyrrhiza glabra root were used to replace Astragalus membranaceus root.

[0061] Centella asiatica is sourced from commercially available dried products.

[0062] Gentian root is sourced from commercially available dried products.

[0063] Purslane is sourced from commercially available dried products.

[0064] Chamomile is sourced from commercially available dried products.

[0065] Licorice root is sourced from commercially available dried products.

[0066] Experiment 1 The hyaluronidase inhibition rate (%) of the test samples prepared from the soothing and anti-inflammatory compositions of each example and comparative example was determined according to T / JSRH 003-2023 "Test of Anti-allergic Activity of Cosmetic Raw Materials - Hyaluronidase Inhibition Method".

[0067] Test sample: 0.3g of soothing and anti-inflammatory composition, 99.7g of deionized water, mixed evenly to obtain the test sample.

[0068] Experiment 2 The assay for inhibiting cellular inflammatory factors follows these steps: Cell seeding: Dilute macrophages (mouse monocytic leukemia cell line RAW264.7, purchased from the Chinese Academy of Sciences Cell Bank, catalog number: TCM13) with cell culture medium to the seeding density (confluence reaches 45%-60% 24h after seeding), seed into 96-well plates, 200μL per well, and incubate in a CO2 incubator for 24h.

[0069] Drug administration: Discard the culture medium in the 96-well plate and proceed with induction and drug administration. Add the test sample and 1 μg / mL LPS culture medium to the test sample wells at a 1:1 volume ratio. Add cell culture medium containing LPS to the negative control wells and cell culture medium to the blank control wells. Add 200 μL of cell culture medium to each well and continue culturing in a CO2 incubator for 24 h.

[0070] Cell supernatant collection: After incubation, collect 200 μL of cell culture supernatant into a 1.5 mL sterile centrifuge tube and store at -80°C.

[0071] ELISA detection: Perform the detection according to the instructions of the TNF-α ELISA detection kit.

[0072] Test sample: 0.3g of soothing and anti-inflammatory composition, 99.7g of deionized water, mixed evenly to obtain the test sample.

[0073] Experiment 3 Several volunteers with sensitive skin were recruited and divided into groups of ten. The groups used the soothing and anti-inflammatory compositions prepared in each example and comparative example to prepare the test samples. They used 5ml once in the morning and once in the evening each time, applying it evenly to the face until absorbed. Before each use, the face was cleansed with water and a disposable face towel. The experiment lasted for 60 days, and no other skin care products were used during the experiment.

[0074] Test sample: 0.3g of soothing and anti-inflammatory composition, 99.7g of deionized water, mixed evenly to obtain the test sample.

[0075] Before the experiment, volunteers cleansed their faces with water and disposable face towels, dried their skin with a clean towel, and then tested their skin moisture content, recording it as the initial moisture content. They then spent 2 hours in a constant temperature (28℃) and constant humidity (65%) environment, during which time their skin moisture content was tested again and recorded as the moisture content after 2 hours. The skin moisture loss over time before skincare was calculated using a formula: Skin moisture loss over time before skincare = initial moisture content - moisture content after 2 hours.

[0076] After the 60-day experiment, volunteers first cleansed their faces with water and disposable face towels, then dried them with a clean towel. The skin's moisture content was then tested and recorded as the initial moisture content after skincare. They were then allowed free movement for 2 hours in a constant temperature (28℃) and constant humidity (65%) environment, and the skin's moisture content was tested again and recorded as the moisture content 2 hours after skincare. The skin's moisture loss over time after skincare was calculated using a formula: Skin moisture loss over time after skincare = initial moisture content after skincare - moisture content 2 hours after skincare.

[0077] The average value of the experimental data for each group was taken.

[0078] When testing skin moisture content, a Corneometer CM825 is used to measure facial skin moisture. The higher the measured value, the higher the skin moisture content.

[0079] The less moisture a skin loses over time, the healthier its skin barrier.

[0080] The detailed experimental data for Experiments 1-3 are shown in Table 1.

[0081] According to the data comparison of each embodiment and comparative example in Table 1, the soothing and anti-inflammatory effects of each embodiment are better than those of each comparative example. It can be seen that each embodiment, by combining saffron, sophora flavescens root, houttuynia cordata, moringa seed and astragalus, can exert a soothing and anti-inflammatory effect higher than that of traditional soothing plant extracts, and can relieve the discomfort caused by skin allergies more quickly.

[0082] Meanwhile, the skin moisture loss over time after skin care in each embodiment was significantly lower than that in the control group. It can be seen that the combination of saffron, sophora flavescens root, houttuynia cordata, moringa seed and astragalus in each embodiment can also play a good role in promoting skin barrier repair, restoring skin health and effectively improving sensitive skin.

[0083] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A method for preparing a soothing and anti-inflammatory composition, characterized in that: Includes the following steps: Step 1) Mix saffron, sophora flavescens root, houttuynia cordata, moringa seeds and astragalus to obtain compound raw materials; Step 2), extract the composite raw materials in an ethanol-water solution, filter, and collect the filtrate; Step 3), concentrate and dry the filtrate to obtain a soothing and anti-inflammatory composition.

2. The method for preparing a soothing and anti-inflammatory composition according to claim 1, characterized in that: In step 1), the mass ratio of saffron, sophora flavescens root, houttuynia cordata, moringa seeds, and astragalus is 3-4:2-3:1-2:1-2:1-2.

3. The method for preparing a soothing and anti-inflammatory composition according to claim 2, characterized in that: In step 1), saffron, sophora root, houttuynia cordata, moringa seeds, and astragalus are mixed and then crushed to obtain a composite raw material of 80-100 mesh.

4. The method for preparing a soothing and anti-inflammatory composition according to claim 2, characterized in that: In step 2), the ethanol aqueous solution contains 70-75% ethanol by mass.

5. The method for preparing a soothing and anti-inflammatory composition according to claim 4, characterized in that: In step 2), the composite raw material is extracted in an ethanol-water solution for 1-2 hours.

6. The method for preparing a soothing and anti-inflammatory composition according to claim 5, characterized in that: In step 2), the composite raw materials are extracted in an ethanol aqueous solution at a constant temperature of 60-70℃ for 1-2 hours.

7. The method for preparing a soothing and anti-inflammatory composition according to claim 6, characterized in that: In step 2), the mass ratio of the composite raw material to the ethanol aqueous solution is 1:10-20.

8. A soothing and anti-inflammatory composition, characterized in that: It is prepared by the method of any one of claims 1-7 for preparing the soothing and anti-inflammatory composition.

9. The application of the soothing and anti-inflammatory composition according to claim 8, characterized in that: The soothing and anti-inflammatory composition is added to skincare product formulations to prepare skincare products with soothing and anti-inflammatory effects.

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