A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology

A surface-enhanced Raman and electrophoretic separation technology, applied in Raman scattering, material analysis by electromagnetic means, measurement devices, etc., can solve problems such as restricting the development of surface-enhanced Raman spectroscopy and hybrid detection, and achieve a significant enhancement effect. , the detection process is simple, the effect of high sensitivity

Active Publication Date: 2022-02-18
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

SERS can detect a single substance, but in the face of more complex detection samples, such as multiple mixtures with similar surface-enhanced Raman spectra and molecular physical properties that are difficult to separate and distinguish, it cannot deal with the messy surface-enhanced Raman mixed together Spectroscopy, which restricts the development of surface-enhanced Raman spectroscopy and hybrid detection

Method used

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  • A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology
  • A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology
  • A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology

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Embodiment

[0022] A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology, comprising the following steps:

[0023] (1) if figure 1 As shown, cut the glass sheet into 1.5cm×7.5cm, add ethanol and wash it in an ultrasonic cleaner for 5 minutes, repeat three times, then dry it with nitrogen gas, and fix it in the deposition chamber; when the pressure is less than 5×10 -7 In the Torr vacuum deposition chamber, through electron beam evaporation, a layer of titanium thin film and silver thin film are successively deposited on the cleaned glass at the speed of 0.2nm / s and 0.3nm / s, and then the sample stage is rotated to make the substrate normal and the deposition The included angle of the direction is 86°. By electron beam evaporation, a 940nm thick silver nanorod array is grown at a speed of 0.3nm / s. The diameter of the silver nanorod is 92nm, and the interval between two adjacent silver nanorods is 95nm.

[0024] (2) if figure 2 As ...

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Abstract

A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology, comprising: a. using oblique angle deposition technology to prepare silver nanorod arrays; b. combining the gel electrophoresis technology, the substrate prepared in step a and gel electrophoresis Combine the instruments; c. Adjust the voltage and current, so that the mixture to be tested is spread out according to different positions on the substrate; d. Use a surface-enhanced Raman spectrometer to detect the surface-enhanced Raman spectra at different positions on the substrate, and distinguish and judge the mixture. different components. The detection method of the present invention has the advantages of simple detection process, high sensitivity, significant enhancement effect, strong repeatability, and large-scale production; it can effectively utilize the characteristics of high efficiency of gel electrophoresis and high detection of silver nanorods, thereby realizing detection of multiple similar mixtures It can be applied to the separation and detection of a variety of similar complex samples.

Description

technical field [0001] The invention relates to a surface-enhanced Raman spectrum detection method, in particular to a surface-enhanced Raman spectrum detection method combined with gel electrophoresis separation technology. Background technique [0002] In recent years, surface-enhanced Raman spectroscopy (surface-enhanced Raman scattering, SERS) amplifies the electromagnetic field by exciting localized surface plasmons, thereby obtaining molecular structure information and sensitive detection limits. SERS is widely considered as a convenient and sensitive analytical tool due to its non-destructive and fingerprint-like resolution ability, weak impact on the water environment, and high sensitivity, and has been applied in many fields such as biological analysis, food safety, and environmental science fields. SERS can detect a single substance, but in the face of more complex detection samples, such as multiple mixtures with similar surface-enhanced Raman spectra and molecul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N27/447
CPCG01N21/658G01N27/447
Inventor 韩彩芹宋宇航李静文
Owner XUZHOU NORMAL UNIVERSITY
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