Method for separating and detecting imipramine and trimipramine

A technology of trimipramine and imipramine, which is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of expensive high-performance liquid chromatography and mass spectrometry instruments, complicated operation, and separation and detection costs Advanced problems, to achieve the effect of high detection sensitivity, simple experimental equipment, simple operation method

Active Publication Date: 2010-08-11
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High-performance liquid chromatography and mass spectrometry instruments are expensive, complicated to operate, and high in separation and detection costs

Method used

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  • Method for separating and detecting imipramine and trimipramine
  • Method for separating and detecting imipramine and trimipramine
  • Method for separating and detecting imipramine and trimipramine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Follow the steps below to prepare NaH 2 PO 4 -Na 2 HPO 4 buffer solution

[0044] Prepare NaH with a concentration of 100mmol / L 2 PO 4 solution and a concentration of 100mmol / L Na 2 HPO 4 solution;

[0045] Mix the above solutions with the same concentration and dilute them with secondary water to make the concentration 50mmol / L, then adjust the pH value of the solution to 7.0.

Embodiment 2

[0047] Follow the steps below to prepare Tris buffer solution

[0048] Preparation concentration is the Tris solution of 100mmol / L;

[0049] Dilute the above Tris solution to 20mmol / L with secondary water;

[0050] with concentrated H 3 PO 4 The solution was adjusted to a 20mmol / L Tris solution to a pH of 2.0.

Embodiment 3

[0052] The device used is: MPI-A type capillary electrophoresis electrochemiluminescence detector, an uncoated fused silica capillary column with an inner diameter of 75 μm, a traditional three-electrode system including a Pt disk working electrode with a diameter of 500 μm, an Ag / AgCl reference electrode, and a Pt wire electrode.

[0053] With the NaH prepared in Example 1 2 PO 4 -Na 2 HPO 4 The buffer solution is used as the background electrolyte, 2mmol / L ruthenium terpyridine is added to the background dielectric, the scanning potential range is 0.20-1.30V, and the cyclic voltammetry curve and the electrochemiluminescence curve are recorded when stable, such as figure 1 a curve and a' curve in

[0054] Add 0.3mmol / L imipramine and trimipramine respectively to the solution containing the above, and the scanning potential range is 0.20-1.30V, and record the cyclic voltammetry curve and electrochemiluminescence curve when stable, such as figure 1 b, c curves and b', c' c...

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Abstract

The embodiment of the invention discloses a method for separating and detecting imipramine and trimipramine. The method comprises the following steps: using a capillary tube as separation channel and a mixed solution of trihydroxymethylaminomethane, beta-cyclodextrin and acetonitrile as separation solution to separate imipramine and trimipramine, and on the end of the capillary tube, using a tris(2,2'-bipyridyl)ruthenium (II) electrochemical luminescence system to detect imipramine and trimipramine to finally realize the separation and detection of imipramine and trimipramine. The method which uses a capillary electrophoresis-electrochemical luminescence combination device to separate and detect imipramine and trimipramine has the advantages of simple experimental devices, easy and practical operation method, low cost, high separation efficiency, high detection sensitivity and the like. When the method provided by the invention is used to separate and detect imipramine and trimipramine, the limit of detection of imipramine and the limit of detection of trimipramine are separately 5.0*10<-9>mol / L and 1.0*10<-9>mol / L.

Description

technical field [0001] The invention relates to the technical field of imipramine and trimipramine, more specifically, to a method for separating and detecting imipramine and trimipramine. Background technique [0002] Imipramine and trimipramine are tricyclic antidepressants. Tricyclic antidepressants have been used to treat depression since the 1960s and are currently also used to treat other psychiatric disorders such as obsessive-compulsive disorder, attention deficit disorder, panic attacks, phobias, chronic pain syndromes, peripheral neuropathy , nocturnal enuresis, anxiety, eating disorders, and migraines. [0003] The current analysis methods include high performance liquid chromatography, gas chromatography, electrochemical methods, capillary electrophoresis, non-aqueous capillary electrophoresis and so on. High performance liquid chromatography has very good separation ability, but the cost is relatively high. Detection techniques include ultraviolet-visible det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 由天艳于彩霞唐小风袁柏青李霞
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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