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Non-contact conductivity detector and detection method

A non-contact conductance and detector technology, used in instruments, measuring devices, scientific instruments, etc., to achieve the effects of easy miniaturization, stable response value, and accurate measurement

Inactive Publication Date: 2005-01-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, at present, the capacitive coupling non-contact conductometric detection (C 4 D) The research has just started, and there is no relevant literature report

Method used

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  • Non-contact conductivity detector and detection method

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Embodiment

[0062] Used for the separation and detection of several inorganic cations by capillary electrophoresis;

[0063] Electrophoresis conditions: separation voltage + I0KV, L = 50cm × 50μm I.D × 375μm O.D, L effi = 40cm, pressure injection 30s, height 10cm, buffer solution is 10mM MES / HIS (pH=5.0, adjusted with 2% acetic acid), the water used is high-purity water, Li + , Na + , K + , Mg 2+ , Ca 2+ , Ba 2+ The concentration is 20μM;

[0064] Detection conditions: the electrode length is 5mm, the distance between the two electrodes is 2mm, the frequency of the sinusoidal alternating current is 20KHz, and the function generator outputs the peak-to-peak voltage V p-p = 50V. Separation spectrum see Figure 5 .

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Abstract

The invention relates to a detection technique for conductance which is a detecting apparatus and method for separation detecting of capillary electrophoresis, electricity chromatogram and microcylinder liquid phase chromatogram. This invention comprises function signal generator, detecting electrode, electrode fixing device, signal processing circuit, wherein the two detecting electrodes are in telescopic tubular shape and are directly covered on both ends of capillary; and electrode fixing device is composed of two boards with notches in and two detecting electrodes are located in the notches and are connected separately with signal processing circuit; the above components are set in one metal shielding box; the guiding means of capillary is fixed on the side wall of the box; and the guiding wire of detecting electrode is led by the guiding hole and thus is connected with output edge of function signal generator. This invention is of simple structure and high accuracy and good utilization.

Description

technical field [0001] The invention relates to a conductivity detection technology, in particular to a detector and a detection method for separation and detection of capillary electrophoresis, electrochromatography and micro-column liquid chromatography. Background technique [0002] Conductivity detectors have been widely used in the separation and detection of capillary electrophoresis. Its structure is simple, and it has advantages in the detection of ionic compounds, especially for the detection of some small inorganic cations and anions with low electrical activity and weak optical absorption by amperometric detection. . Conductivity detectors for capillary electrophoresis detection generally use a pair of platinum (Pt) microelectrodes directly inserted into the laser-drilled capillary or placed at the end of the separation capillary, or a metal film is deposited at the end of the capillary as the detection electrode. A certain frequency AC signal is applied to the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N30/64
Inventor 关亚风谭峰王海龙
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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