Positive-phase liquid chromatogram-tandem mass spectrum detection method for nicotine optical isomers in tobacco and tobacco products
A technology of normal phase liquid chromatography and optical isomers, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long analysis time, difficulty in accurate quantification of R-(+)-nicotine, and low sensitivity. To achieve the effect of short analysis time, improved sensitivity and high sensitivity of the method
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Embodiment 1
[0024] (1) Pretreatment of tobacco leaf samples: Accurately weigh 0.30 g cut tobacco, add it into a 50 mL Erlenmeyer flask with a stopper, add 2 mL of 5.0% NaOH solution, let it stand for 10 min, and accurately add d 3 - 20 mL of nicotine racemate in n-hexane, racemic d 3 -The concentration of nicotine was 5 mg / mL, ultrasonically extracted for 15 min, the supernatant was passed through the organic phase filter membrane with 2.0 g of anhydrous sodium sulfate in the syringe, and the filtrate was analyzed by normal phase liquid chromatography-tandem mass spectrometry.
[0025] (2) Instrumental analysis conditions: Normal phase liquid chromatography-tandem mass spectrometry is used for analysis, and the chromatographic column used is Daicel chiral chromatographic column CHIRALPAK ® IC, the size is 4.6 mm×250 mm, i.d. 5 µm; column temperature: 30 o C; mobile phase A: n-hexane containing 0.1% diethylamine, mobile phase B: isopropanol; 95%A+5%B isocratic elution; flow rate 1.0 mL / m...
Embodiment 2
[0027] Five kinds of tobacco leaf samples were selected, and the content of nicotine optical isomers in the samples was determined according to the analysis method described in Example 1, and the results are shown in Table 3.
[0028] Table 3 Contents of nicotine optical isomers in tobacco leaf samples (mg / g)
[0029] Sample serial number S-(-)-nicotine R-(+)-nicotine 1# 14.30 0.036 2# 16.32 0.049 3# 19.60 0.045 4# 18.30 0.049 5# 18.42 0.052
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