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Mobile phase for separating phospholipids in high efficiency liquid chromatography

A high-performance liquid chromatography and mobile phase technology, applied in the field of organic chemical analysis, can solve problems such as system leakage, and achieve the effects of good use, high sensitivity, and simple operation

Active Publication Date: 2009-09-23
西安血氧生物技术有限公司
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Problems solved by technology

Among them, the phosphoric acid / sulfuric acid in the mobile phase composed of acetonitrile-methanol-phosphoric acid / sulfuric acid will produce precipitation, which will cause scratches on the plunger and gasket of the high-pressure pump in the high-pressure liquid chromatography, resulting in system leakage

Method used

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  • Mobile phase for separating phospholipids in high efficiency liquid chromatography
  • Mobile phase for separating phospholipids in high efficiency liquid chromatography
  • Mobile phase for separating phospholipids in high efficiency liquid chromatography

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Embodiment Construction

[0028] The specific implementation and effect of the distribution ratio of the components of the mobile phase of the present invention will be described below in conjunction with the accompanying drawings, and the specific chromatographic conditions for the separation and detection of the mobile phase of the present invention will be described.

[0029] High performance liquid chromatography includes system controller, ultraviolet detector, high performance liquid phase pump system, degassing system and column thermostat. The chromatographic column is a reversed-phase analytical column C18 (250mm×4.6mm I.D.), the drift tube temperature of the evaporative light scattering detector is 80°C, the nitrogen flow rate is 2L / min; the mobile phase flow rate is 1.0ml / min.

[0030] Separation and detection experiment initially uses HPLC-UV to separate and detect phospholipids. The sample is the phospholipids extracted from porcine erythrocyte membranes. The volume ratio of the mobile phas...

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Abstract

A mobile phase for separating phospholipids in high performance liquid chromatography, the composition of the mobile phase of the present invention includes: 10% to 50% of methanol, 50% to 85% of acetonitrile, 0.5% to 3% of fluorinated organic acid, and 0.5 to 3% of water %. The mobile phase of the present invention does not produce any precipitation through long-term use, does not cause damage to the pump system, and does not have any damage to the pump system of the liquid chromatograph; it can be used to detect separated phospholipids in an evaporative light scattering detector, Compared with the UV detector, the detected baseline is stable, easy for quantitative analysis, high sensitivity, and the application of its mobile phase is not subject to any restrictions; compared with the gradient method, the separation and detection can be realized by using a single pump; When proportioning, in addition to effectively separating phospholipids, it can also separate unknown substances that cannot be separated by other mobile phases. When the mobile phase of the present invention is used for isocratic elution, the operation is simple, time-saving, reproducible and easy to repeat.

Description

technical field [0001] The invention relates to a mobile phase used in the separation of phospholipids by high performance liquid chromatography, and belongs to the technical field of organic chemical analysis. Background technique [0002] Methods for separating phospholipids by high performance liquid chromatography include isocratic elution and gradient elution. Generally use high-performance liquid chromatography and ultraviolet detector combination (HPLC-UV) to detect phospholipids, high-performance liquid chromatography provides the mobile phase with stable flow rate and higher pressure to the chromatographic column, so that the sample is separated at the chromatographic column, and then The separated phospholipids were detected with a UV detector. If the proportion of each solvent in the mobile phase is constant during detection, it is an isocratic elution method, and if the instrument automatically mixes different solvents according to requirements, it is a gradient...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/08
Inventor 严坤平但宁陈超
Owner 西安血氧生物技术有限公司
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