Preparation method of strong cation exchange chromatographic filler for glycosylated hemoglobin

A technology of strong cation exchange and glycosylated hemoglobin, which is applied in the direction of cation exchange materials, cation exchange, ion exchange, etc., can solve the problems of complex preparation process, achieve separation detection, simple and controllable preparation method, avoid graded treatment or seed swelling Effect

Pending Publication Date: 2021-11-19
WUXI CITY KAIAOSHAN BIOPHARML TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The seed polymerization method requires pre-preparation of small-sized polymer particles, which are swollen and grown with various monomers, initiators and other small molecular compounds, and then initiated polymerization at high temperature. The preparation process is relatively complicated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Initially polymerize methyl methacrylate to form monodisperse microspheres with a particle size of 2-15 μm; use a three-necked flask to weigh 10 parts of methyl methacrylate, 3 parts of azobisisobutyronitrile, 10 parts Parts of polyvinyl alcohol and 77 parts of ethanol were uniformly mixed by ultrasonic and placed in a water bath, the stirrer was turned on to keep the reaction system uniform, nitrogen gas was introduced for 10 minutes to remove oxygen in the device, and the temperature of the water bath was raised to 70°C to start the reaction.

[0041] Step 2: cross-linking and polymerizing methyl methacrylate to expand the particle size of the microspheres; use a beaker to weigh 5 parts of methyl methacrylate, 2 parts of ethylene glycol dimethacrylate, and 2 parts of azo Diisobutyronitrile and 5 parts of polyvinyl alcohol were dissolved evenly by ultrasonication. After the polymerization system in Step 1 reacted for 3 hours, it was added into the reaction syst...

Embodiment 2

[0045]Step 1: perform preliminary polymerization on methyl methacrylate to form monodisperse microspheres with a particle size of 2-15 μm; use a three-necked flask to weigh 13 parts of methyl methacrylate, 4 parts of azobisisobutyronitrile, 10 parts of polyvinyl alcohol, 70 parts of ethanol and 3 parts of purified water were mixed uniformly by ultrasonic and placed in a water bath. The stirrer was turned on to keep the reaction system uniform, and nitrogen gas was introduced for 10 minutes to remove the oxygen in the device. Raise to 70°C and start the reaction.

[0046] Step 2: Methyl methacrylate is further cross-linked and polymerized to expand the particle size of the microspheres; use a beaker to weigh 7 parts of methyl methacrylate, 3 parts of ethylene glycol dimethacrylate, 4 parts of even Nibisobutyronitrile and 10 parts of polyvinyl alcohol were dissolved evenly by ultrasonication. After the polymerization system in step 1 reacts for 3 hours, add it into step 1; cont...

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Abstract

The invention provides a preparation method of a strong cation exchange chromatographic filler for glycosylated hemoglobin. The preparation method comprises the steps of step 1, carrying out primary polymerization on a first monomer to form microspheres with a monodisperse particle size; step 2, further cross-linking and polymerizing the first monomer to enlarge the particle size of the microspheres; step 3, further introducing a second monomer for polymerization, and polymerizing functional groups on the microspheres; and step 4, cleaning, and removing the solvent, the dispersing agent, redundant monomers and the like in the reaction system. According to the invention, the defects in the prior art are overcome, the design is reasonable, the structure is compact, and the monodisperse strong cation exchange chromatographic filler with the particle size of 2-15 microns is obtained through a multi-step dispersion polymerization method. The complex operations such as staged treatment or seed swelling and microsphere post-modification in the synthesis process of the existing filler synthesis method can be avoided, and the preparation method is simpler and more controllable. The strong cation exchange chromatographic filler obtained by adopting a multi-step dispersion polymerization method can realize separation and detection of glycosylated hemoglobin.

Description

technical field [0001] The invention relates to the technical field of preparation of glycosylated hemoglobin chromatographic packing, in particular to a preparation method of strong cation exchange chromatographic packing for glycosylated hemoglobin. Background technique [0002] Glycosylated hemoglobin (GHb) is the product of the combination of hemoglobin in red blood cells and carbohydrates in serum. It is formed through a slow, continuous and irreversible glycation reaction, and its content depends on the blood sugar concentration and the contact time between blood sugar and hemoglobin, but has nothing to do with blood drawing time, whether the patient is fasting, whether insulin is used or not. GHb is composed of HbA1a, HbA1b, and HbA1c, of which HbA1c accounts for about 70% and has a stable structure. At present, HbA1c is the "gold standard" for evaluating the long-term blood sugar control status of diabetic patients, and has important value in the treatment of diabete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J39/18B01J39/19B01J39/20C08F220/14C08F222/14C08F220/58
CPCB01J39/18B01J39/19B01J39/20C08F220/14C08F222/102C08F220/585
Inventor 冯巧芳李俊彭伟邹丽洁林滔刘岩
Owner WUXI CITY KAIAOSHAN BIOPHARML TECH
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