136 results about "Glycated Hb" patented technology

A non-enzymatic electrochemical method of simultaneous measurement of hemoglobin (Hb) and percentage of glycated hemoglobin (% HbA1c) in a blood sample is disclosed. The method includes determining the total amount of hemoglobin in a sample by electrochemically measuring the voltammetric current due to iron (II) and iron (III) redox portions in hemoglobin and determining the percentage of glycated hemoglobin (HbA1c) by potentiometry. Also disclosed is a novel screen-printed electrode (SPE) strip modified for potentiometric measurement of HbA1c.

The invention discloses a method for measuring glycosylated hemoglobin and a kit. The kit comprises a kit body, reagent bottles, a bottle mat, a hinge and a kit cap, wherein the kit body is connected with the kit cap through the hinge; the four reagent bottles are respectively used for placing a magnetic particle suspension, an erythrocyte splitting liquor, a flushing liquid and signals. According to the invention, magnetic particles capable of quantitatively capturing glycosylated hemoglobin and non-glycosylated hemoglobin in a sample are adopted, and percentage of the glycosylated hemoglobin in the sample to the total hemoglobin can be calculated only by detecting the content of glycosylated hemoglobin on the magnetic particles and comparing the content with the standard work curve. According to the invention, the operation is simple, and the quantitative detection on the percentage composition of the glycosylated hemoglobin can be completed only with one operation; and biological raw materials such as antibodies are not needed, so that the price is low, and detecting instrument miniaturization is easy to realize.

The invention discloses recombinant human fibroblast growth factor 21 fusion protein and application thereof in preparation of medicine for treating metabolic diseases. A nucleotide sequence of coding genes of the recombinant human fibroblast growth factor 21 fusion protein and an expression vector are connected to obtain a recombinant expression vector; the recombinant expression vector converts host cells, high-expression positive host cells are screened out, the cells are cultured, the recombinant human fibroblast growth factor 21 fusion protein is induced to be expressed, thalli are collected, breaking, centrifuging, clarifying and purifying are carried out, and the target product recombinant human fibroblast growth factor 21 fusion protein is obtained. The invention further discloses the application of the recombinant human fibroblast growth factor 21 fusion protein in preparation of the medicine for treating metabolic diseases. The recombinant human fibroblast growth factor 21 fusion protein can well control blood sugar fluctuation and remarkably improve the level of glycosylated hemoglobin and triglyceride.

There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and / or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.

The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase. The amount of hydrogen peroxide generated in the reaction is measured for determination of percentage of glycated hemoglobin in blood samples. The invention provides kits for performing the methods of the invention.

The invention relates to a glycosylated hemoglobin immunochromatographic test strip and a preparation method thereof and a kit. The test strip comprises a substrate, and a sampling pad, a coating filmand an absorption pad which are arranged on the substrate, wherein the sampling pad, the coating film and the absorption pad are sequentially connected from one end of the substrate to the other endof the substrate; a marking area, a detection area and a quality control area are sequentially arranged on the coating film; the marking area is coated with a coupling compound marked by fluorescent latex particles, and the coupling compound is formed by coupling a first dog and cat HbA1c monoclonal antibody and a first complex; the detection area is coated with a second dog and cat HbA1c monoclonal antibody; the quality control area is coated with a second complex, and the first complex and the second complex can be specifically combined; and the first dog and cat HbA1c monoclonal antibody can be specifically combined with hemoglobin, and the second dog and cat HbA1c monoclonal antibody can be specifically combined with glycosylated hemoglobin. The glycosylated hemoglobin immunochromatographic test strip provided by the invention achieves quantitative detection of glycosylated hemoglobin of dogs and cats, and has the characteristics of rapidness and simplicity.

By way of summary, the present invention meets some or all of the above needs by providing in a first aspect a method comprising contacting red blood cells in a suspension medium containing mannose under conditions such that the concentration of S-Alc glycated hemoglobin is increased to greater than about 6 percent by weight of the hemoglobin in the red blood cells. Preferably the suspension medium is an aqueous suspension medium. In preferred embodiments, the method is performed at about ambient temperature, 18° C. to about 23° C.

The invention relates to a test method of glycosylated hemoglobin, comprising the following steps of: by utilizing the specific adsorption of ion resin to hemoglobin, firstly preparing a hemoglobin sample, fully mixing ion resin with the prepared hemoglobin sample evenly at the temperature of 25-40 DEG C to completely adsorb hemoglobin not glycosylated in blood and keeping glycosylated hemoglobin in blood in liquid, then measuring absorbance values of the liquid and whole blood after adsorption treatment, and obtaining the content of glycosylated hemoglobin according to the ratio of the treated liquid to the treated absorbance value. According to the invention, a microcolumn chromatography is changed to a liquid homogeneous phase adsorption method of separation, so that the operation time is greatly shortened, the operation is simple, the labor intensity of operators is reduced, and the time that a patient waits for a result is shortened; and a disposable plastic cuvette or small bottle is used as a reaction vessel, thus reducing errors and improving degree of precision. The application range is wider, the test method and the test kit can be conveniently used in inspection departments, medical wards and basic medical institutions.

This invention provides an amadoriase having high reactivity with α-fructosyl hexapeptide (αF6P) derived from the β-chain amino terminus of glycated hemoglobin (HbAlc), which enables satisfactory quantification of αF6P cleaved from HbAlc. A novel amadoriase is derived from the amadoriase of the genus Coniochaeta by substitution of one or more amino acids at positions selected from the group consisting of positions 62, 63, 102, 106, 110, 113, 355, and 419. Such amadoriase enables rapid, simple, accurate, and satisfactory quantification of αF6P cleaved from HbAlc. With the use of such amadoriase, a method for measurement of HbAlc by an enzymatic method and a kit for measurement of HbAlc that are based on the same principle as used in the conventional standard method and excellent in terms of the relevance with the standard method.