Blood routine examination and immunochromatography combined detection reagent card

Abstract

The invention discloses a blood routine examination and immunochromatography combined detection reagent card, and belongs to the technical field of medical detection, the blood routine examination and immunochromatography combined detection reagent card comprises a reagent card body, the reagent card body comprises a detection counting area, a reagent liquid filling area and a sampling area, the sampling area is used for accommodating a sample and pre-diluting the sample, the reagent liquid filling area is used for treating and diluting the sample, and the detection counting area is used for counting the detection counting area. The detection counting area comprises a red blood cell counting square groove, a white blood cell counting square groove and a blood platelet counting square groove. Five groups of neutrophil, lymphocyte, mononuclear cell, eosinophilic granulocyte and basophilic granulocyte in leukocyte are realized, and promotion of a basic POCT instrument can be promoted by matching with other necessary blood routine parameters and CRP, SAA, glycosylated hemoglobin and other parameters. The operation of a user is convenient, the joint detection test of blood routine examination and immunochromatography items can be fully automatically completed by one reagent card and the immunochromatography card, and extra dilution of a sample is not needed.

Description

Technical Field

[0001] The invention belongs to the technical field of medical detection, and in particular is a blood routine and immunochromatography combined detection reagent card. Background Art

[0002] Routine blood tests usually include 25 indicators, mainly including three-category or five-category white blood cell counts, red blood cell counts, platelet counts and hemoglobin concentrations. Immunochromatographic tests mainly include fluorescent immunochromatographic tests such as CRP and SAA and colloidal gold tests for influenza A and influenza B.

[0003] At present, routine blood tests usually require the use of a blood cell analyzer. Traditional blood analyzer detection methods include electrical impedance method and sheath flow scattering method, which have the advantages of fast detection speed and accuracy, but have the disadvantages of requiring maintenance of the liquid circuit, high instrument cost and large size. With the implementation of the tiered diagnosis and treatment policy, more and more grassroots medical institutions such as clinics, health centers, dental clinics and beauty hospitals have more demand for routine blood tests. However, grassroots medical institutions have the characteristics of small sample size, lack of professional testing personnel, and tight laboratory space, which makes traditional blood analyzers unable to meet their needs.

[0004] The single-portion blood analyzer based on POCT (timely diagnosis) can perfectly meet the needs of primary medical institutions. It has the characteristics of no liquid path, maintenance-free, no pollution, small size and low instrument cost. There are two principles of blood analyzers based on POCT technology. One is the single-portion electrical impedance method. The gem micropores are made into plastic parts through mold injection molding, which become disposable consumables, and then installed between the two electrodes of the reagent card. The blood cell categories and quantities are counted by detecting the changes in the current pulse when the blood cells flow through the micropores. The principle is consistent with the traditional Coulter method. However, this method can only achieve three-classification detection of white blood cells, and because the assembly of micropores and electrodes is relatively complicated, the cost of single-portion reagent card consumables is high, which is difficult for customers to accept, and there is a risk of micropore blockage. The other is the single-portion image analysis method. After the cells are hemolyzed and stained, the processed samples are added to a cavity with a height of 100-200 microns, and photographed after precipitation, and then the classification and statistics are automatically completed by the image algorithm. The image analysis method has the advantages of low consumables cost and the ability to perform five classifications of white blood cells. The current image analysis-based instruments all manually process samples, add samples and cell sedimentation, and most of them only perform image photography of white blood cells. In addition, routine blood tests are usually combined with immune tests at the same time, so that clinical diagnosis can be better performed on whether the patient is infected with bacteria or viruses.

[0005] At present, POCT blood analysis instruments perform blood routine test and immune test separately. Patent with publication number CN111912766A proposes a test card for combined immune test and cell test. The reagent card disclosed in the patent requires manual sample processing and sample addition, and can only perform white blood cell test, not a complete blood routine test. In clinical evaluation, only white blood cell test has no clinical significance. Moreover, when calculating immune parameters such as CRP / SAA, the real hematocrit whole blood parameter is not used, and the theoretical set value is used. The result obtained will be different from the actual hematocrit value of special population, resulting in erroneous test results. Summary of the invention

[0006] The object of the present invention is to provide a blood routine test and immunochromatography combined detection reagent card to solve the above-mentioned problems in the prior art.

[0007] Provide a blood routine and immunochromatography combined detection reagent card, including:

[0008] The reagent card body includes a detection and counting area, a reagent filling area and a sampling area. The sampling area is used to accommodate samples and pre-dilution of samples. The reagent filling area is used for sample processing and dilution. The detection and counting area includes a red blood cell counting square groove, a white blood cell counting square groove and a platelet counting square groove.

[0009] This design integrates multiple blood processing and testing functions into one reagent card, which can perform routine blood tests and immunochromatography tests at the same time, simplifying the testing process, improving the adaptability of primary medical institutions to routine blood tests, and reducing equipment costs.

[0010] As a further embodiment of the present invention: it also includes a quantitative sampler, the sampling area includes a sampling hole, the quantitative sampler is detachably connected to the reagent card body, and the quantitative sampler is used to inject the sample into the sampling hole. This design allows the sampling process to control the sample volume and improves the accuracy of the test. Since the quantitative sampler can be separated from the reagent card, the convenience and reliability of the operation are improved, which meets the rapid detection requirements of the POCT device.

[0011] As a further embodiment of the present invention: the quantitative sampler is penetrated by a discharge hole. The discharge hole on the quantitative sampler can be used in conjunction with a TIP head. The hollow straw of the TIP head automatically sucks the sample into the tube by capillary force, and the TIP head is inserted into the discharge hole. The instrument's electric pipette pump provides power to discharge the sucked sample into the sampling hole below. Then, the TIP head absorbs a certain volume of diluent, aligns it with the discharge hole, and discharges the diluent by air pressure. The diluent enters the sampling hole from the discharge hole, flushing out the sample in the hollow capillary, and repeats this process many times until it is flushed clean.

[0012] As a further embodiment of the present invention: the sampling area includes a sample adding hole. The sample adding hole is used to directly add venous blood, and when venous blood has been collected, it is used to store the blood sample transferred from the blood collection tube.

[0013] As a further embodiment of the present invention: a counting area cover is provided on the detection counting area. The counting area cover can seal the counting area to prevent external pollutants from interfering with the detection process, facilitate microscope observation, and ensure the accuracy of the test.

[0014] As a further embodiment of the present invention: the bottoms of the square grooves for red blood cell counting, white blood cell counting and platelet counting are provided with light-transmitting areas for arranging microscope illumination light sources.

[0015] As a further embodiment of the present invention: the square grooves for red blood cell counting, white blood cell counting and platelet counting are bonded to the counting area cover plate by ultraviolet light curing glue. The closed design of the counting area cover plate helps to prevent the sample from overflowing during the detection process.

[0016] As a further embodiment of the present invention: the counting area cover plates on the red blood cell counting square trough, the white blood cell counting square trough and the platelet counting square trough are integrally formed. The integrally formed structure reduces the number of parts in the assembly process and improves assembly efficiency. The closed design of the counting area cover plate helps prevent the sample from overflowing during the detection process.

[0017] As a further embodiment of the present invention: the counting area cover is provided with a red blood cell detection cavity exhaust hole connected to the red blood cell counting square groove and a red blood cell reaction liquid loading groove, the counting area cover is provided with a white blood cell detection cavity exhaust hole connected to the white blood cell counting square groove and a white blood cell reaction liquid loading groove, and the counting area cover is provided with a platelet detection cavity exhaust hole connected to the platelet counting square groove and a platelet reaction liquid loading groove.

[0018] The liquid adding groove is located at one end of the square groove and is used to draw the liquid to flow in the detection cavity of the square groove. The exhaust hole is located at the other end of the square groove and is used to exhaust air to balance the air pressure in the detection cavity of the square groove, so that the liquid can flow smoothly.

[0019] As a further embodiment of the present invention, the reagent filling area includes a hemoglobin processing liquid chamber, and the side wall of the reagent card body opposite to the hemoglobin processing liquid chamber is provided with a hemoglobin detection light-transmitting surface. The hemoglobin detection light-transmitting surface can be used to perform absorbance detection on the sample in the hemoglobin processing liquid chamber.

[0020] As a further embodiment of the present invention: the reagent filling area includes a red blood cell processing liquid chamber, a second diluent chamber, a first diluent chamber, a platelet processing liquid chamber, a red blood cell diluent chamber, an inflammation processing liquid chamber, a saccharification processing liquid chamber, a white blood cell processing liquid chamber and a white blood cell reaction chamber. The reagent filling area includes a plurality of different chambers, which can provide a special processing liquid for the sample. This multi-chamber design ensures that the sample is scientifically and reasonably processed during the detection process, and can be tested for various components separately, thereby realizing the combined detection of blood routine and immune detection, and improving the comprehensiveness and accuracy of the detection.

[0021] As a further embodiment of the present invention: a mounting portion is provided at the bottom of the reagent card body, and the mounting portion is used to ensure that the reagent card is stably placed when in use.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. It realizes the five groups of neutrophils, lymphocytes, monocytes, eosinophils and basophils in white blood cells, and cooperates with other necessary blood routine parameters and CRP, SAA, glycosylated hemoglobin and other parameters to promote the promotion of grassroots POCT instruments.

[0024] 2. Convenient operation for users. One reagent card plus an immunochromatography card can automatically complete the combined test of blood routine and immunochromatography items without additional sample dilution. The all-in-one design reduces the detection cost of a single card. Combined with microscopy and image recognition technology, the instrument is liquid-free and maintenance-free, which meets the application requirements of POCT usage scenarios and reduces instrument and detection costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present drawings or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present drawings. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0026] Figure 1 This is a schematic diagram of the overall structure of the combined detection reagent card provided by the present invention;

[0027] Figure 2 An exploded diagram of the structure of the combined detection reagent card provided by the present invention;

[0028] Figure 3 This is a partial structural schematic diagram of the combined detection reagent card provided by the present invention.

[0029] In the figure: 1, reagent card body; 107, sampling hole; 115, red blood cell counting square slot; 116, white blood cell counting square slot; 117, platelet counting square slot; 101, mounting part; 102, red blood cell treatment liquid chamber; 103, diluent second chamber; 104, diluent first chamber; 105, platelet treatment liquid chamber; 106, hemoglobin treatment liquid chamber; 107, sampling hole; 108, sample addition hole; 109, red blood cell dilution liquid chamber; 110, inflammation treatment liquid chamber; 111, saccharification treatment liquid chamber; 112, white blood cell treatment Liquid cavity; 113, hemoglobin detection light-transmitting surface; 114, white blood cell reaction cavity; 115, red blood cell counting square groove; 116, white blood cell counting square groove; 117, platelet counting square groove; 118, groove; 2, quantitative sampler; 201, discharge hole; 3, counting area cover; 301, red blood cell detection cavity exhaust hole; 302, white blood cell detection cavity exhaust hole; 303, platelet detection cavity exhaust hole; 304, red blood cell reaction liquid sample adding groove; 305, white blood cell reaction liquid sample adding groove; 306, platelet reaction liquid sample adding groove. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is described and illustrated below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments provided by the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of the present invention.

[0031] Obviously, the drawings described below are only some examples or embodiments of the present invention. For ordinary technicians in this field, the present invention can also be applied to other similar scenarios based on these drawings without creative work. In addition, it can also be understood that although the efforts made in this development process may be complicated and lengthy, for ordinary technicians in this field related to the content disclosed by the present invention, some changes in design, manufacturing or production based on the technical content disclosed by the present invention are just conventional technical means, and should not be understood as insufficient content disclosed by the present invention.

[0032] However, unnecessary detailed descriptions may be omitted. For example, detailed descriptions of well-known matters and repeated descriptions of substantially the same structures may be omitted. This is to avoid the following description from becoming unnecessarily lengthy and to facilitate understanding by those skilled in the art. In addition, the drawings and the following description are provided to enable those skilled in the art to fully understand the present invention and are not intended to limit the subject matter described in the claims.

[0033] See also Figure 1-Figure 3As shown, the blood routine and immunochromatography combined detection reagent card in the embodiment of the present invention includes a reagent card body 1, and the reagent card body 1 includes a detection and counting area, a reagent filling area and a sampling area. The sampling area is used to accommodate samples and pre-dilution of samples, the reagent filling area is used for sample processing and dilution, and the detection and counting area includes a red blood cell counting square groove 115, a white blood cell counting square groove 116 and a platelet counting square groove 117.

[0034] There are three counting windows in total, namely, the square slot 115 for red blood cell counting, the square slot 116 for white blood cell counting, and the square slot 117 for platelet counting. The square slots are separated from each other to achieve independence. A counting area cover plate 3 is provided on the detection and counting area. The counting area cover plate 3 is made of a transparent material, and various holes are designed to guide the liquid to the corresponding square slot area, and then perform microscopic photography and immune detection. A light-transmitting area is provided at the bottom of the square slot 115 for red blood cell counting, the square slot 116 for white blood cell counting, and the square slot 117 for platelet counting, where a microscope illumination light source can be arranged.

[0035] The quantitative sampler 2 is used to collect quantitative samples and discharge the samples into the sampling hole 107. The quantitative sampler 2 is penetrated by a discharge hole 201, and the discharge hole 201 on the quantitative sampler 2 can be used in conjunction with the TIP head. The hollow straw of the quantitative sampler 2 automatically sucks the sample into the tube by capillary force, and the TIP head is inserted into the discharge hole 201. The instrument's electric pipette pump provides power to discharge the sample sucked by the quantitative sampler 2 into the sampling hole 107 below. After the sample of the quantitative sampler 2 is discharged, it is installed on the sampling hole 107 and fixed by the groove 118. The TIP head absorbs a certain volume of diluent, then aligns it with the discharge hole 201, and discharges the diluent by air pressure. The diluent enters from the discharge hole 201 and flushes out the sample in the hollow capillary of the quantitative sampler 2, and repeats several times until it is flushed clean.

[0036] The sampling area includes a sample addition hole 108, which is used to store blood samples transferred from the blood collection tube to the reagent card for testing when venous blood has been collected. The sample addition hole 108 is suitable for directly adding venous blood therein, and does not require the quantitative sampler 2 for quantitative blood collection.

[0037] A counting area cover plate 3 is provided on the detection counting area. The counting area cover plate 3 is made of transparent material and is used to stabilize the samples in each square groove, prevent external contamination, and create conditions for microscope observation.

[0038] The counting area cover plate 3 and the reagent card body 1 can be bonded by ultraviolet light curing glue. The counting area cover plate 3 on the red blood cell counting square groove 115, the white blood cell counting square groove 116 and the platelet counting square groove 117 are integrally formed. The counting area cover plate 3 is provided with a red blood cell reaction solution loading groove 304, a white blood cell reaction solution loading groove 305 and a platelet reaction solution loading groove 306 which are connected to the corresponding square grooves. The reaction solution to be tested can be siphoned to the detection cavity area of ​​the three corresponding square grooves through the loading grooves. The detection cavity of the square groove is formed by being recessed inward.

[0039] The counting area cover plate 3 is provided with a red blood cell detection chamber exhaust hole 301, a white blood cell detection chamber exhaust hole 302, and a platelet detection chamber exhaust hole 303 which are connected to the corresponding square grooves. The three detection chamber areas have independent exhaust holes. When the reaction liquid flows into the sample loading slot, the gas is discharged through the exhaust hole located at the other end of the counting area cover plate 3 to balance the internal air pressure, so as to realize the flow of the traction liquid in the detection chamber.

[0040] The three detection areas of the red blood cell counting slot 115, the white blood cell counting slot 116 and the platelet counting slot 117 correspond to the following three tests respectively:

[0041] The red blood cell counting square slot 115 is mainly used for measuring red blood cell parameters;

[0042] The white blood cell counting square slot 116 is mainly used for total white blood cell count and five-category grouping calculation of neutrophils, lymphocytes, monocytes, eosinophils, and basophils;

[0043] The platelet counting slot 117 is mainly used for measuring platelet parameters.

[0044] The red blood cell treatment liquid chamber 102, the white blood cell treatment liquid chamber 112, the red blood cell dilution liquid chamber 109, the platelet treatment liquid chamber 105, the diluent chamber 104, the diluent chamber 2 103, the hemoglobin treatment liquid chamber 106, the inflammation treatment liquid chamber 110, and the saccharification treatment liquid chamber 111 are all reagent filling areas and need to be sealed with a heat-sealed film. The white blood cell reaction chamber 114, the sample addition hole 108, and the sampling hole 107 are all non-filling areas.

[0045] The bottom of the reagent card body 1 is provided with a mounting portion 101 , which may specifically be a T-slot structure, so as to facilitate fixing the detection reagent card in the detection instrument.

[0046] The specific implementation method is:

[0047] 1. The quantitative sampler 2 draws a quantitative whole blood sample, preferably 30ul, and inserts it into the mounting position of the quantitative sampler 2 of the reagent card, i.e., the sampling hole 107.

[0048] 2. Pre-dilute the sample 1:6.

[0049] 2.1. Insert the reagent card into the instrument. Use the pipette tip to draw 150ul of diluent into the diluent chamber 104 , and flush the sample through the discharge hole 201 of the quantitative sampler 2 into the sampling hole 107 .

[0050] 2.2. Then aspirate 120ul of the liquid in the sampling hole 107 , flush the remaining sample into the sampling hole 107 , and then enter the sampling hole 107 .

[0051] 2.3. Repeat step 2.2 several times to rinse out the remaining sample, preferably 6 times.

[0052] 2.4 The TIP head descends to the mixing position in the cavity of the sampling hole 107, and the sample and the diluent are fully mixed by suction and discharge for multiple times, and the number of suction and discharge is preferably 6 times;

[0053] 3. Sample processing after pre-dilution: Divide the sample into 4 parts and process the red blood cells, white blood cells, platelets, hemoglobin and immunochromatography samples separately.

[0054] 3.1. Red blood cell sample processing: Aspirate not less than 15ul of pre-diluted sample from the sampling hole 107, preferably 20ul, and add it to the red blood cell processing liquid chamber 102 for sample dilution and mixing. After the processing, aspirate not less than 15ul of processed sample from the red blood cell processing liquid chamber 102, preferably 20ul, and add it to the red blood cell reaction solution adding groove 304 for cell precipitation.

[0055] 3.2. Leukocyte sample processing: draw no less than 15ul of pre-diluted sample from the sampling hole 107 into the leukocyte reaction chamber 114, preferably 30ul, and then draw 30ul of leukocyte processing solution from the leukocyte processing solution chamber 112 into the leukocyte reaction chamber 114 to mix with the sample. After the processing, draw 20ul of the treated sample and add it into the leukocyte reaction solution adding groove 305 for cell precipitation.

[0056] 3.3. Platelet sample processing: draw no less than 15ul of pre-diluted sample from the sampling hole 107, preferably 30ul, and add it to the platelet processing liquid chamber 105 for sample mixing. After processing, draw 15ul of the processed sample from the platelet processing liquid chamber 105 and add it to the platelet reaction solution loading groove 306 for cell precipitation.

[0057] 3.4. After the three treatment liquids in 3.1 to 3.3 are added to their respective sample adding slots, the liquids are quickly and evenly filled with the entire cavity to be tested by the capillary force of the inner wall of the square slot cavity. In order to prevent air residue from hindering the flow of liquid until the entire detection area is filled, three air outlets play the role of exhausting air.

[0058] 3.5. Hemoglobin sample processing: Aspirate no less than 15ul of pre-diluted sample from the sampling hole 107, preferably 20ul, and add it into the hemoglobin processing liquid chamber 106 for sample mixing. After the processing, perform absorbance detection through the hemoglobin detection light-transmitting surface 113.

[0059] 3.6. Immune sample processing: Absorb not less than 40ul of the processed red blood cell reaction solution in the red blood cell processing liquid chamber 102, preferably 50ul, and add it to the inflammation processing liquid chamber 110 for sample mixing. After processing, absorb not less than 60ul of the processing solution from the inflammation processing liquid chamber 110, preferably 100ul, and add it to the immunochromatography reagent strip for immunochromatography inflammation project detection. Or absorb not less than 40ul of the processed hemoglobin reaction solution in the hemoglobin processing liquid chamber 106, preferably 50ul, and add it to the saccharification processing liquid chamber 111 for sample mixing. After processing, absorb not less than 60ul of the processing solution from the saccharification processing liquid chamber 111, preferably 100ul, and add it to the immunochromatography reagent strip for immunochromatography saccharified hemoglobin project detection. The reaction liquid will be absorbed by the sample pad on the immunochromatography reagent strip in turn, develop a color reaction on the analysis membrane, and be absorbed on the absorbent pad to form a flow traction force. The immunochromatographic reagent strip is an existing reagent strip used for immunochromatographic detection, and the specific structure will not be described in detail.

[0060] 4. Take photos under a microscope and perform immunoassay on the detection and counting area.

[0061] 4.1. The precipitation and reaction time in the detection counting area is not less than 2 minutes and not more than 5 minutes, preferably 3.5 minutes.

[0062] 4.2. Take photos of red blood cells, white blood cells and platelets in turn on the red blood cell counting slot 115, the white blood cell counting slot 116 and the platelet counting slot 117.

[0063] 4.3. After the immune reaction, color development or fluorescence detection is performed.

[0064] 5. Image analysis and result calculation.

[0065] It should be noted that the present invention is not limited to the above-mentioned embodiments. The above-mentioned embodiments are only examples, and the embodiments having the same structure as the technical idea and exerting the same effect within the scope of the technical solution of the present invention are all included in the technical scope of the present invention. In addition, without departing from the scope of the main purpose of the present invention, various modifications that can be thought of by those skilled in the art to the embodiments and other methods of combining some of the constituent elements in the embodiments are also included in the scope of the present invention.

Claims

1. A blood routine test and immunochromatography combined detection reagent card, characterized in that: include: The reagent card body (1) comprises a detection and counting area, a reagent filling area and a sampling area. The sampling area is used to contain samples and pre-dilution of samples. The reagent filling area is used for sample processing and dilution. The detection and counting area comprises a red blood cell counting square groove (115), a white blood cell counting square groove (116) and a platelet counting square groove (117).

2. A blood routine test and immunochromatography combined detection reagent card according to claim 1, characterized in that: It also includes a quantitative sampler (2), the sampling area includes a sampling hole (107), the quantitative sampler (2) is detachably connected to the reagent card body (1), and the quantitative sampler (2) is used to inject the sample into the sampling hole (107).

3. A blood routine test and immunochromatography combined detection reagent card according to claim 2, characterized in that: The quantitative sampler (2) is penetrated by a discharge hole (201).

4. A blood routine test and immunochromatography combined detection reagent card according to claim 1, characterized in that: The sampling area includes a sample loading hole (108).

5. A blood routine test and immunochromatography combined detection reagent card according to claim 1, characterized in that: A counting area cover plate (3) is provided on the detection counting area.

6. A blood routine test and immunochromatography combined detection reagent card according to claim 5, characterized in that: The red blood cell counting square groove (115), the white blood cell counting square groove (116) and the platelet counting square groove (117) are bonded to the counting area cover plate (3) by ultraviolet light curing glue.

7. A blood routine test and immunochromatography combined detection reagent card according to claim 6, characterized in that: The counting area cover plate (3) is provided with a red blood cell detection chamber exhaust hole (301) and a red blood cell reaction solution loading groove (304) which are connected to the red blood cell counting square groove (115); the counting area cover plate (3) is provided with a white blood cell detection chamber exhaust hole (302) and a white blood cell reaction solution loading groove (305) which are connected to the white blood cell counting square groove (116); and the counting area cover plate (3) is provided with a platelet detection chamber exhaust hole (303) and a platelet reaction solution loading groove (306) which are connected to the platelet counting square groove (117).

8. A blood routine test and immunochromatography combined detection reagent card according to claim 1, characterized in that: The reagent filling area includes a hemoglobin processing liquid chamber (106), and a hemoglobin detection light-transmitting surface (113) is provided on the side wall of the reagent card body (1) opposite to the hemoglobin processing liquid chamber (106).

9. A blood routine test and immunochromatography combined detection reagent card according to claim 1, characterized in that: The reagent filling area includes a red blood cell processing liquid chamber (102), a second diluent chamber (103), a first diluent chamber (104), a platelet processing liquid chamber (105), a red blood cell diluent chamber (109), an inflammation processing liquid chamber (110), a saccharification processing liquid chamber (111), a white blood cell processing liquid chamber (112) and a white blood cell reaction chamber (114).

10. A blood routine test and immunochromatography combined detection reagent card according to claim 1, characterized in that: A mounting portion (101) is provided at the bottom of the reagent card body (1).

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