Method for purifying preadipocytes derived from fetal bovine skeletal muscle tissue
A fat cell and skeletal muscle technology, applied in the field of biochemistry, can solve the problems of reducing fat in the body and not being able to obtain it
Active Publication Date: 2017-01-04
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology
Given the many articles published each year on lipid metabolism and adipogenesis, it is largely impossible to obtain an effective method of reducing body fat or reducing adipocyte differentiation through exogenous treatments
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Embodiment 1
[0052] A method for purifying preadipocytes derived from bovine fetal skeletal muscle tissue, the material used in the method is longissimus dorsi muscle tissue of bovine 3-month-old fetus, and the reagents used are collagenase, fetal bovine serum, low-sugar Dulbecco Modified Eagle's medium, cell sorting buffer, anti-platelet-derived growth factor receptor alpha antibody, dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin and oil red O.
[0053] The method comprises the steps of:
[0054] Step 1, isolation and culture of cells;
[0055] Step 2, cell culture;
[0056] Step 3, adipogenic induction of cells;
[0057] Step 4, Oil Red O;
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The invention discloses a method for purifying preadipocytes derived from fetal bovine skeletal muscle tissue. According to the method, a used material is longissimus dorsi tissue of a 3-month-old bovine fetus, and used reagents contain collagenase, fetal bovine serum, the low- glucose Dulbecco's Modified Eagle's Medium, a cell sorting buffer solution, a PDGFR-alpha (platelet-derived growth factor receptor alpha) antibody, dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin and oil red O.
Description
technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for purifying preadipocytes derived from bovine fetal skeletal muscle tissue. Background technique [0002] Stem cells are a type of cells that can maintain the ability to replicate and differentiate into different types of cells. The study of stem cells is very important for understanding the regulation of the occurrence and differentiation of cells and tissues. In addition, stem cell research also has great potential for the medical field. Like many other organs, skeletal muscle contains many cell types that give rise to myogenic satellite cells and adipose tissue-derived adipocytes, both of which are important for animal production. Satellite cells are known to be important for both postnatal skeletal muscle growth and adult skeletal muscle regeneration. Research on isolated satellite cells over the past 50 years has focused on the activation and in...
Claims
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Login to View More IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2500/40C12N2501/135C12N2501/33C12N2509/00
Inventor 张路培胡鑫高会江李俊雅
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI