62 results about "Skeletal Muscle Tissue" patented technology

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and / or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and / or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

The present invention provides compositions and methods for treating and / or preventing diseases and disorders associated with expression of PPAR γ and / or infiltration of macrophages into skeletal muscle tissue and / or white adipose tissue. The method treats such diseases and disorders with abscisic acid (ABA). Exemplary diseases and disorders include diabetes, including type 2 diabetes, prediabetes, glucose intolerance insulin resistance, and diseases and disorders involving the immune system, such as inflammation, including obesity-related inflammation, inflammatory bowel disease, type 1 diabetes, multiple sclerosis, allergies, asthma, cardiovascular disease, and arthritis.

A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and / or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.

A method and apparatus capable of measuring visceral fat tissues accumulated in the trunk with high accuracy apply a current to a body part where a subcutaneous fat tissue layer is thin or a body part where a skeletal muscle tissue layer has no or a thin muscle belly portion from a pair of current applying electrodes, measure a potential difference which has occurred in the tissue through which the current has passed by a pair of voltage measuring electrodes, and determine the visceral fat tissue volume of the trunk by use of the impedance of the trunk which has been obtained by use of the measured potential difference.

The present disclosure provides electroporation systems and methods of preconditioning tissue for electroporation therapy. An electroporation generator includes an electroporation circuit, a preconditioning circuit, and a controller. The electroporation circuit is configured to be coupled to a catheter for delivering the electroporation therapy to target tissue of the patient. The electroporation circuit is further configured to transmit an electroporation signal through the catheter. The preconditioning circuit is configured to be coupled to a preconditioning electrode for stimulating skeletal muscle tissue of the patient. The preconditioning circuit is further configured to transmit a preconditioning signal to the preconditioning electrode. The controller is coupled to the electroporation circuit and the preconditioning circuit, and is configured to synchronize transmissions of the electroporation signal and the preconditioning signal such that the preconditioning signal is transmitted prior to transmission of the electroporation signal.

The regenerative potential of aged stem cells is enhanced by activation of the Notch signaling pathway and / or inhibition of TGF-β signaling pathway. Stem cells in aged tissues are capable of proliferation and tissue regeneration, but in their native setting are not provided with the appropriate signals to do so. By administering tissue regenerating agents, organ stem / progenitor cells are provided with the appropriate signals to regenerate the corresponding differentiated tissues.

This invention relates to one skeleton muscle slice dyeing method, which comprises the following steps: taking skeleton muscles into fix liquid for four to six hours and then cutting the muscles into organism blocks for three to five millimeter and then putting into fix liquid for eight to eighteen hours; taking skeleton muscles organism block flow by clearing and into potassium permanganate dyeing liquid for ten to twelve days; then using alcohol liquid with seventy percent for 15 to 30 minutes for clearing orderly through alcohol liquid with 70, 80 and 90 percent into eosin dyeing liquid; then putting the skeleton muscles organism into alcohol for 95 percent and 100 percent alcohol for two hours and dipping the organism block into dimethylbenzene and then into soft wax twice and hard wax for twice.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and / or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and / or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and / or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.

The invention discloses a method for purifying preadipocytes derived from fetal bovine skeletal muscle tissue. According to the method, a used material is longissimus dorsi tissue of a 3-month-old bovine fetus, and used reagents contain collagenase, fetal bovine serum, the low- glucose Dulbecco's Modified Eagle's Medium, a cell sorting buffer solution, a PDGFR-alpha (platelet-derived growth factor receptor alpha) antibody, dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin and oil red O.

Techniques are provided which can isolate pluripotent stem cells at high purity capable of differentiation into at least a myocardial cell to regenerate the cardiac muscle. The pluripotent stem cells at high purity capable of differentiation into at least a myocardial cell to regenerate the cardiac muscle can be isolated through the following steps: (i) collecting a skeletal muscle tissue from a mammal and enzymatically treating the obtained skeletal muscle tissue to prepare a skeletal muscle tissue-derived cell; (ii) culturing the obtained skeletal muscle tissue-derived cell in a culture medium containing an epidermal growth factor and a fibroblast growth factor; (iii) selecting and separating a colony that is floating in the culture medium.

The invention discloses a disposable and rapid octadecyl-chain-containing targeting mitochondrion fluorescent probe. The fluorescent probe is a carbazole pseudo-indole salt compound of a structure shown in a formula (I). The compound can achieve disposable and rapid imaging of mitochondria in living cells and tissue. Particularly, in rat skeletal muscle tissue, the probe can achieve clear imaging of mitochondrion reticular structures of four different mitochondria, and can give a three-dimensional high-definition image of distribution of mitochondrion in the tissue. The probe can track dynamic changes of the mitochondrial morphology in cells in real time. Compared with a mitochondrion fluorescent probe with similar functions, the fluorescent probe has the advantages of being low in cost, good in light stability and good in biocompatibility. The probe also has the high mitochondria positioning capability and hypotoxicity, has the good biocompatibility with Hochest 33342, MTG and MTR, and has the potential application value in the field of laser induced fluorescence biomarkers.

The invention discloses a method for purifying myoblast derived from bovine fetus skeletal muscle tissue. The method is characterized in that the material used by the method is the longissimus dorsi muscle tissue of a 3-month-old bovine fetus, and reagents used by the method are horse serum, anti-fast muscle myosin heavy chain antibodies, collagenase, fetal bovine serum, a low-sugar Dulbecco modified Eagle medium, a cell-sorting buffer solution and anti-platelet derived growth factor receptor alpha antibodies.

The invention relates to construction of an efficient double promoter PLEGFP-N1-spMyoD1 green fluorescence retrovirus vector, belonging to the technical fields of animal gene engineering and animal molecule breeding engineering. The construction of the efficient double promoter PLEGFP-N1-spMyoD1 green fluorescence retrovirus vector is characterized by artificially synthesizing a skeletal muscle specific promoter sequence, using a RT-PCR method to amplify a gene overall length sequence from a skeletal muscle tissue of a pig, designing a fusion primer, using an overlapped PCR amplification method to obtain the promoter and a fusion gene sequence, cloning T / A to a pMD18-T vector, constructing the efficient expression vector of the PLEGFP-N1-spMyoD1 double promoter, and identifying with restriction enzyme and sequencing, packaging with a liposome method and amplifying, and monitoring virus titer and efficiency of infection. The invention has the beneficial effects: 1, Primer Premier 5.0, DNA Club and Oligo 6.0 are utilized to self-design the primer; 2, the restriction that an objective gene can not be subject to specific tissue expression in the past is overcome; 3, pertinency and accuracy of study on the objective gene functions are simultaneously improved; and 4, detection is fast and convenient, and has strong reliability.

The invention belongs to biotechnology pharmacy technology field. The recombination plasmid Pci-Hpon3 expressed in zooblast is constructed by used human mintacol enzyme 3 gene (Hpon3), Hpon3 is expressed efficiently and secreted into blood in small murine skeletal muscles organizing in electric transferring method to improve PON3 in blood serum and enforce the ability of small mouse antioxidation pressure so that it can control liver damage efficiently. The activity of Hpon3 transgene expression small mouse blood serum hydrolytic decomposition melilotol is improved by 1.4 times and can maintain 24 days. After small mouse acute or subacute liver damage mould is built by injecting carbon tetrachloride into abdominal cavities of transgene group and normal small mouse, the blood serum ALT and AST level of transgene small mouse are lower greatly, malondialdehyde (MDA) level of liver homogenate is lower, reducing glutathione (GSH) and total antioxidant ability (T-AOC) are higher than contrasting group, and transgene group small mouse liver cell damage extent indicated in nosology slicer result is lower than CC1(4) control group's indicate that recombination human PON3 plasmid transgene expression can improve efficiently blood serum PON3 activity and reduce liver oxygenizing pressure so that it can be used for preventing and treating liver damage.

Use of idebenone for the preparation of a medicament for the treating of a muscular dystrophy in particular for treating and / or preventing weakness and / or loss of skeletal muscle tissue and / or cardiomyopathy associated with a muscular dystrophy.

The invention provides a skeletal muscle tissue frozen section myosin adenosine triphosphatase (ATPase) dyeing kit which comprises a CaCl2-containing buffer solution, an acetate buffer solution, a substrate incubation solution, 2wt% of cobalt chloride solution and 1wt% of ammonium sulfide solution. In the invention, a skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit is established, thus, the use is convenient, the working efficiency is improved, and the reporting period of a case is shortened; moreover, the reagent is saved, the cost is remarkably lowered, and a stable and satisfactory result is obtained.

A medical device, a method for preparation thereof, and use thereof are provided. The medical device comprises a thermoplastic elastomer that is composed of soft segments and hard segments. The method for preparing a medical device comprising a thermoplastic elastomer, comprises forming the thermoplastic elastomer into tubing or other shapes via extrusion, molding, or coating, assembling the tubing or other shapes with other parts including: cables, coils, coated cables, or coated coils, and bonding the tubing, cables, or coils with other components including: other tubing components, cables, coils, sleeves, electrical pulse generator, defibrillation shock generator, electrodes, sensors, or drug release components. The medical device is used for correcting cardiac rhythm, defibrillating, assisting hearts, sensing, stimulating neurological systems, gastrointestinal system, or skeletomuscular tissues or organs.

The invention relates to the field of tissue engineering and regenerative medicine and designs a novel trilayer tissue repair film by constructing collagen-modified single-row high-molecular ordered fibers and bacterial cellulose and modifying the finished film with a static magnetic field generated by an added magnetic patch. An inner layer of the tissue repair film is made with the collagen-modified single-row high-molecular ordered fibers based on the action of fiber morphology upon cell behaviors, so that a binding site is provided for cells, and polar growth of the cells is induced. An intermediate layer is made with the bacterial cellulose, so that cell penetration is facilitated and a survival space is preserved for the cells. an outermost layer is made with the magnetic patch; thestatic magnetic field generated by the magnetic patch can produce a series of biological effects upon cells of living things. The novel trilayer tissue repair film is used mainly to regenerate skin tissue and repair skeletal muscular tissue. Based on structural optimization, the novel trilayer tissue repair film can be applied to tissue wounds, so that collected cells are regulated through the collagen-modified single-row high-molecular ordered fibers. finally, the behaviors of different cells in different tissue repair phases can be regulated through the series of biological effects which theapplied added magnetic field produces for the cultured cells.

The invention relates to a human body skeleton muscle tissue stress-strain nondestructive analysis method based on in-vivo physiological movement. The method is composed of a human body movement capturing and analyzing system, a ground reaction force collecting and analyzing system, a muscle force solving system and a skeleton muscle system stress-strain analyzing system. Firstly, the motion capture system is used for capturing the posture of in-vivo three-dimensional physiological motion of a human body, kinematics data of all joints of the human body are extracted through data processing, then foot ground reaction force during in-vivo physiological motion of the human body is collected and analyzed through the ground reaction force collection and analysis system, and a muscle force solution is obtained through the muscle force solution system. The joint force and the muscle force under the corresponding movement are solved and calculated, and finally kinematics data, dynamics data and the muscle force are input into a skeleton and muscle tissue stress-strain analysis system, so that calculation and analysis of the stress-strain of the skeleton and muscle tissue under the in-vivo physiological movement are realized. According to the invention, nondestructive analysis of the stress and strain of the skeletal muscle tissue of the human body under the physiological movement condition is realized.

The invention relates to an adipose tissue forming method based on in-vitro induction of a vascularized pedicle. The adipose tissue forming method comprises the following steps: taking an adipofascialtissue of the vascularized pedicle as a living support, inducing seed cells with mature adipocytes as main bodies in situ, performing in-vitro perfusion and transdifferentiation by using a muscle forming culture solution to obtain skeletal muscle cells, and constructing an axial vascularized tissue engineering skeletal muscle tissue in vitro. The adipose tissue forming method has the beneficial effects: under the premise of alleviating body damage to the maximum degree, the axial vascularized tissue engineering skeletal muscle tissue is cultured for treating muscle defects.

The invention belongs to the technical field of biology and in particular relates to a promoter for specifically expressing a gene in poultry skeletal muscle and application. The promoter for specifically expressing the gene in the poultry skeletal muscle, provided by the invention, comprises at least one E-box; the nucleotide sequence of the E-box CANNTG, wherein N is selected from A, T, C or G.The invention further provides application of the gene promoter capable of being specifically expressed in the poultry skeletal muscle. The promoter for specifically expressing the gene in the poultryskeletal muscle, provided by the invention, can be used for constructing various specific transgenic expression vectors of the poultry skeletal muscle; and the specific transgenic expression vectorsare used for high-abundance expression of the promoter gene in poultry skeletal muscle tissue or skeletal muscle derived cells or the poultry meat quality and muscle-related disease therapy are improved through a gene engineering technology.

Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and / or number of multinucleated cells) of said organized skeletal muscle tissue on said support.

The invention relates to a novel pharmacological action of a traditional Chinese medicine monomer salvianolic acid A, and applications of the salvianolic acid A in preparing products for preventing, relieving and / or treating including muscular atrophy, myopathy, musculoskeletal complications and complications thereof caused by various reasons including diabetes, heredity, muscular dystrophy and neurological dysfunction. The salvianolic acid A has the advantages that: the salvianolic acid A has the effects of delaying and treating muscular atrophy caused by various reasons and diabetes, increasing skeletal muscle strength, improving skeletal muscle tissue microcirculation, increasing skeletal muscle weight, and improving pathological injury of skeletal muscle tissues. The salvianolic acid Ais a monomer compound extracted from danshen roots, has low toxicity and wide raw material resources, has good application and development prospects, and can be used for preventing, relieving and treating muscular atrophy, diabetic myopathy, diabetic muscular atrophy, diabetic musculoskeletal complications and complications thereof caused by various reasons.

The invention relates to a novel pharmacological action of a traditional Chinese medicine monomer puerarin. The novel pharmacological action comprises prevention, relief and / or treatment of myopathy,muscular atrophy and musculoskeletal complications caused by metabolic diseases including diabetes, hyperlipidaemia, obesity and thyroid diseases. The puerarin has the following advantages: the puerarin has the effects of delaying and treating muscular atrophy caused by related metabolic diseases like diabetes, hyperlipidaemia, obesity and thyroid diseases; the puerarin has the pharmacological effects of increasing skeletal muscle strength, improving skeletal muscle tissue microcirculation, increasing skeletal muscle weight and improving skeletal muscle peripheral neuropathy; the puerarin is amonomeric compound extracted from kudzuvine root, is low in toxicity and wide in raw material resource, and has good application and development prospects; and the puerarin can be used for preventing, relieving and treating muscular atrophy, myopathy and musculoskeletal complications caused by related metabolic diseases like diabetes, hyperlipidaemia, obesity and thyroid diseases.

The invention relates to a system and control method of biological cell stimulation based on annularly-distributed electrodes. The system comprises the annularly-distributed electrodes which can be assembled in a biological culture dish and a multi-channel bidirectional pulse power source. The system provided by the invention can realize the functions of promoting cell growth and differentiation and regulating and controlling muscle cells and myotube contraction movement by electric stimulation, and can realize detection of cell electrical stimulation response characteristic when the system cooperates with a cell beating detection device; because the system can realize the functions of promoting the cell growth and differentiation, regulating and controlling the cell contraction movement,and detecting the cell electrical stimulation response characteristics in situ, complicated processing and labelling of cell samples are not required, and the system can realize high-flux, non-invasive and non-destructive cell culture and detection, so that the system has important potential applications in the field of life sciences, such as myocardium, skeletal muscle tissue engineering, drug screening, and research on life-like micro-nano robots based on muscle cell drive.