Method for purifying myoblast derived from bovine fetus skeletal muscle tissue

A technology of myoblasts and skeletal muscle, applied in the field of agricultural biochemistry, can solve the problems of reducing body fat and inability to obtain

Active Publication Date: 2017-01-25
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Given the many articles published each year on lipid metabolism and adipogenesis, it is largely impossible to...

Method used

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  • Method for purifying myoblast derived from bovine fetus skeletal muscle tissue
  • Method for purifying myoblast derived from bovine fetus skeletal muscle tissue
  • Method for purifying myoblast derived from bovine fetus skeletal muscle tissue

Examples

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Embodiment 1

[0064] A method for purifying bovine fetal skeletal muscle tissue-derived myoblasts, the material used in the method is bovine 3-month-old fetal longissimus dorsi muscle tissue, and the reagents used are horse serum and anti-fast muscle myosin heavy chain antibody , collagenase, fetal bovine serum, low-sugar Dulbecco's modified Eagle's medium, cell sorting buffer, anti-platelet-derived growth factor receptor alpha antibody.

[0065] The method comprises the steps of:

[0066] Step 1, isolation and culture of cells;

[0067] Step 2, cell culture;

[0068] Step 3, myogenic induction of cells;

[0069] Step 4, immunofluorescence;

[0070] Step five, observation of myogenic induction results.

[0071] Described step one specifically comprises the following steps:

[0072] Step (1) washing the bovine fetus with 75% alcohol and phosphate buffer saline containing double antibody for 3-6 times;

[0073] Step (2) Then place it in a sterile environment and cut the amniotic membrane,...

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Abstract

The invention discloses a method for purifying myoblast derived from bovine fetus skeletal muscle tissue. The method is characterized in that the material used by the method is the longissimus dorsi muscle tissue of a 3-month-old bovine fetus, and reagents used by the method are horse serum, anti-fast muscle myosin heavy chain antibodies, collagenase, fetal bovine serum, a low-sugar Dulbecco modified Eagle medium, a cell-sorting buffer solution and anti-platelet derived growth factor receptor alpha antibodies.

Description

technical field [0001] The invention belongs to the technical field of agricultural biochemistry, and in particular relates to a method for purifying myoblasts derived from bovine fetal skeletal muscle tissue. Background technique [0002] Stem cells are a type of cells that can maintain the ability to replicate and differentiate into different types of cells. The study of stem cells is very important for understanding the regulation of the occurrence and differentiation of cells and tissues. In addition, stem cell research also has great potential for the medical field. Like many other organs, skeletal muscle contains many cell types that give rise to myogenic satellite cells and adipose tissue-derived adipocytes, both of which are important for animal production. Satellite cells are known to be important for both postnatal skeletal muscle growth and adult skeletal muscle regeneration. Research on isolated satellite cells over the past 50 years has focused on the activati...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2501/135C12N2501/734C12N2501/998C12N2509/00
Inventor 张路培胡鑫高会江李俊雅
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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