Adipose tissue forming method based on in-vitro induction of vascularized pedicle

An adipose tissue and vascular pedicle technology, which is applied in the field of surgical medicine, can solve the problem that the quality of new skeletal muscle cannot meet the needs of clinical repair of large-scale skeletal muscle defects, and achieves a problem that is conducive to the repair of muscle defects, easy to control conditions, and fully differentiated. Effect

Pending Publication Date: 2018-05-04
PEKING UNIV THIRD HOSPITAL
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Problems solved by technology

[0004] In order to solve the technical defect of using simple skeletal muscle satellite cell inoculation mentioned in the background technology, although skeletal muscle can be formed, it cannot meet the clinical needs of repairing large-scale skeletal muscle defects in terms of the quantity and quality

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  • Adipose tissue forming method based on in-vitro induction of vascularized pedicle
  • Adipose tissue forming method based on in-vitro induction of vascularized pedicle
  • Adipose tissue forming method based on in-vitro induction of vascularized pedicle

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[0030] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other implementations obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0031] The present invention uses fat fascia tissue with vascular pedicles as a living body support, uses a perfusion bioreactor to promote nutrient exchange in vitro, and simulates the mechanical environment in vivo to maintain long-term survival of large volume fat fascia tissue in vitro. In the adipose fascia tissue perfused with myogenic culture medium, the adipose-derived mesenchymal stem cells and mature adipocytes in it will be induced to differentiate in...

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Abstract

The invention relates to an adipose tissue forming method based on in-vitro induction of a vascularized pedicle. The adipose tissue forming method comprises the following steps: taking an adipofascialtissue of the vascularized pedicle as a living support, inducing seed cells with mature adipocytes as main bodies in situ, performing in-vitro perfusion and transdifferentiation by using a muscle forming culture solution to obtain skeletal muscle cells, and constructing an axial vascularized tissue engineering skeletal muscle tissue in vitro. The adipose tissue forming method has the beneficial effects: under the premise of alleviating body damage to the maximum degree, the axial vascularized tissue engineering skeletal muscle tissue is cultured for treating muscle defects.

Description

technical field [0001] The invention belongs to the technical field of surgical medicine, and in particular relates to a method for inducing muscle formation of adipose tissue with vascular pedicles in vitro. Background technique [0002] There is significant loss of muscle tissue and its repair is often caused by a variety of natural disasters, accidental injuries, firearm injuries, etc. It is a more difficult problem in clinical practice. The current clinical treatment is mainly through autologous muscle tissue transplantation as a form and function replacement. However, this method has limited available muscle and causes damage to the donor site, resulting in insufficient muscle volume or loss of function at the donor site. Therefore, there is an urgent need to study methods that can obtain a large amount of tissue-engineered muscle tissue in a short period of time, in order to meet the needs of mass and large-scale muscle tissue defect repair. With the in-depth study ...

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2533/90
Inventor 安阳李东陈鹿嘉聂芳菲薛红宇秦泽莲李比
Owner PEKING UNIV THIRD HOSPITAL
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