System and control method of biological cell stimulation based on annularly-distributed electrodes

A technology of biological cells and control methods, which is applied in the field of variable biological cell stimulation electric field, can solve the problems that cannot be used to study the electrical stimulation response and control characteristics of living muscle cells/tissues, and can not be changed, so as to achieve non-destructive cell culture and detection , The effect of small local high current area and uniform electric field distribution

Pending Publication Date: 2019-11-22
SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the base electrode can reduce the damage to the medium and cells, but once the electrode is fabricated, the direction of the electric field becomes invariable, so it cannot be used to study the electrical stimulation response and control properties of living muscle cells / tissues

Method used

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  • System and control method of biological cell stimulation based on annularly-distributed electrodes
  • System and control method of biological cell stimulation based on annularly-distributed electrodes
  • System and control method of biological cell stimulation based on annularly-distributed electrodes

Examples

Experimental program
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Embodiment 1

[0036] 1) Take frozen myoblasts (C2C12), after recovery, use 5 ml of cell proliferation medium (89% volume of high-sugar DMEM medium + 10% volume of fetal bovine serum + 1% volume of penicillin streptomycin Mixed solution) is cultivated in a cell culture dish with a diameter of 60 mm at a density of 10,000 cells per milliliter, and cultivated at 37 degrees Celsius in a cell culture incubator with 5% carbon dioxide;

[0037] 2) Replace the new cell proliferation medium every day until the cells proliferate to cover 80% of the bottom area of ​​the culture dish;

[0038] 3) Aspirate the cell proliferation medium in the culture dish, wash the cells 3 times with sterile phosphate-buffered saline (PBS), for 3 minutes each time, add cell differentiation medium (97% volume of high-sugar DMEM medium+2 % volume of horse serum+1% volume of penicillin-streptomycin mixture), continue to cultivate at 37 degrees Celsius in a cell incubator with 5% carbon dioxide;

[0039] 4) After culturing...

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PUM

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Abstract

The invention relates to a system and control method of biological cell stimulation based on annularly-distributed electrodes. The system comprises the annularly-distributed electrodes which can be assembled in a biological culture dish and a multi-channel bidirectional pulse power source. The system provided by the invention can realize the functions of promoting cell growth and differentiation and regulating and controlling muscle cells and myotube contraction movement by electric stimulation, and can realize detection of cell electrical stimulation response characteristic when the system cooperates with a cell beating detection device; because the system can realize the functions of promoting the cell growth and differentiation, regulating and controlling the cell contraction movement,and detecting the cell electrical stimulation response characteristics in situ, complicated processing and labelling of cell samples are not required, and the system can realize high-flux, non-invasive and non-destructive cell culture and detection, so that the system has important potential applications in the field of life sciences, such as myocardium, skeletal muscle tissue engineering, drug screening, and research on life-like micro-nano robots based on muscle cell drive.

Description

technical field [0001] The invention relates to a biological cell stimulation system capable of generating electric fields in any direction and in multiple modes, specifically combining the electric field uniformity and direction controllability of annular distributed electrodes, and the separation and synchronization of arbitrary channels of multi-channel bidirectional pulse power supply Controllable and variable biological cell stimulating electric field. It is mainly used for the promotion of growth and differentiation by electrical stimulation of biological cells, the regulation of muscle cell contraction and the detection of electrical stimulation response characteristics. It has important potential applications in the field of life sciences, such as cardiac muscle, skeletal muscle tissue engineering, drug screening, and muscle cell-driven Unit for the study of life-like robots. Background technique [0002] The life-like robot system formed by the organic deep integra...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12N13/00
CPCC12M35/02C12N13/00
Inventor 刘连庆张闯王文学于鹏
Owner SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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