Skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit

An adenosine triphosphate and frozen section technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of waste of reagents, poor dyeing effect, time-consuming and other problems, and achieve the effect of improving work efficiency, convenient effect and saving reagents.

Inactive Publication Date: 2017-05-31
FUJIAN MEDICAL UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in practical application: due to the repeated use of the dyeing solution, the dyeing effect is poor, and the reagents are required to be prepared immediately after use, and the pH of the solution should be precisely adjusted; however, the amount of reagents should not be too small for the dip dyeing method and the detection of pH value. , which is time-consuming and a waste of reagents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit
  • Skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit
  • Skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Myosin adenosin triphosphate (ATPase) staining kit for frozen sections of skeletal muscle tissue, the kit contains the following components:

[0038] (1) CaCl 2 Buffer solution (No. 1 solution): 0.1mol / L glycine solution, 0.1mol / L NaCl solution, 1 mol / LCaCl 2 , 0.1 mol / L NaOH, adjust the pH value to 9.5±0.01 with 0.1 mol / L NaOH;

[0039] (2) Acetate buffer solution (No. 2 solution): 0.2 mol / L sodium acetate, 0.2 mol / L acetic acid, adjust the pH value to 4.6±0.01 with 0.1 mol / L HCl;

[0040] (3) Substrate incubation solution (No. 3 solution): Take the CaCl in No. 1 solution 2 10ml of buffer solution, 25mg of ATP-disodium salt, adjust the pH to 9.4±0.01 with 0.1mol / L NaOH;

[0041] (4) 2wt.% cobalt chloride solution (No. 4 solution);

[0042] (5) Ammonium sulfide solution (No. 5 solution): Ammonium sulfide working solution: 1wt% ammonium sulfide solution, freshly prepared before use.

[0043] No. 1-3 liquids should be stored frozen at -20°C, -35°C or -80°C; liquids 4...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a skeletal muscle tissue frozen section myosin adenosine triphosphatase (ATPase) dyeing kit which comprises a CaCl2-containing buffer solution, an acetate buffer solution, a substrate incubation solution, 2wt% of cobalt chloride solution and 1wt% of ammonium sulfide solution. In the invention, a skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit is established, thus, the use is convenient, the working efficiency is improved, and the reporting period of a case is shortened; moreover, the reagent is saved, the cost is remarkably lowered, and a stable and satisfactory result is obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a myosin adenosin triphosphate (ATPase) staining kit for frozen sections of skeletal muscle tissue. Background technique [0002] Myosin ATPase is localized in skeletal muscle. Skeletal muscle fibers are divided into type I muscle and type II muscle. Type II muscle fibers are divided into IIA and IIB. Under normal circumstances, types I, IIA, and IIB are distributed in a mosaic pattern. Pathological changes should be considered in the following cases of ATPase staining: grouping of muscle fiber types, dominance of a certain type of fiber, and disordered distribution of a certain type of fiber; atrophy or hypertrophy of a certain type of fiber, changes in the size and shape of muscle fibers such as rounded or cord-like; Undifferentiated type IIC myofibers appear. Normal skeletal muscle of infants before 4-5 years old has type IIC muscle fibers, which are undifferentiated ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6887G01N2333/46G01N2333/4712G01N2800/10
Inventor 高美钦刘文文陈裕庆晋雯张文敏
Owner FUJIAN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap