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Preparation method of phencyclidine artificial antigen

An artificial antigen, phencyclidine technology, applied in the direction of peptide preparation methods, chemical instruments and methods, specific peptides, etc., can solve the problems of expensive instruments, time-consuming detection, and unreachable, and achieve advanced synthesis technology and strong specificity Effect

Active Publication Date: 2013-09-25
杭州博林生物技术有限公司
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Problems solved by technology

[0005] At present, the detection of phencyclidine mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), mass spectrometry (MS), etc., but there are expensive instruments, time-consuming detection, and professional The operation by technicians cannot meet the fast and accurate requirements of modern detection

Method used

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  • Preparation method of phencyclidine artificial antigen
  • Preparation method of phencyclidine artificial antigen
  • Preparation method of phencyclidine artificial antigen

Examples

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Embodiment 1

[0033] (1) Preparation of artificial hapten:

[0034] (a) Weigh 2.024g (11.5mmol) of 1-phenylcyclohexanol into a 100ml three-neck round bottom flask, add 40ml of chloroform, and stir at room temperature until the solid is completely dissolved; another 1.740g (23mmol) of sodium azide is weighed Add it to the above reaction solution, cool it to about 0°C with an ice bath, start to slowly add 5ml of trifluoroacetic acid, remove the ice bath after the addition, and stir at room temperature for 5 hours. After the reaction, use concentrated ammonia water to adjust the pH to about 9. After standing and stratifying, the organic phase is washed with double distilled water and saturated aqueous sodium chloride solution. The organic layer is distilled under reduced pressure to obtain 2.023 g (10 mmol) of azide-containing compounds. . Thin-layer chromatography: the chromatographic solution is n-hexane:ethyl acetate=4:1, the product R f =0.7~0.8.

[0035](b) Weigh 0.570g of lithium al...

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Abstract

The invention provides a preparation method of a phencyclidine artificial antigen. The preparation method comprises the following steps of: 1, preparing and detecting hapten: utilizing 1-phenylcyclohexanol as a raw material, introducing azido by using sodium azide, reducing the azido by using lithium aluminum hydride to prepare 1-phenylcyclohexane and then reacting with glutaric anhydride to obtain carboxyl group-containing hapten; and 2, preparing and detecting artificial antigen: combining the artificial antigen with bovine serum albumin (BSA) through a carbodiimide method to prepare the phencyclidine artificial antigen, i.e., phencyclidine-bovine serum albumin. The prepared phencyclidine artificial antigen can be used for animal immunology; a corresponding phencyclidine antibody is obtained; the phencyclidine artificial antigen can be used for research of various phencyclidine immunoassay methods; and a more convenient, quick and accurate approach is provided for detecting the phencyclidine.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of phencyclidine artificial antigen. Background technique [0002] Phencyclidine (pHencycliding, PCP) is a psychoactive drug that has inhibitory, exciting, analgesic and hallucinogenic effects on the central nervous system. Its chemical name is 1-(1-phenylcyclohexane)piperidine. The structural formula is: [0003] [0004] PCP is a dissociative drug used by anesthesiologists as an anesthetic with hallucinogenic and neurotoxic effects. Often called angel powder, it often causes an acute syndrome of disorientation, agitation, and delirium. Although the initial neurological effects of PCP last only a few hours, complete plasma elimination from the body is prolonged, often over several weeks. PCP has potential effects on the nervous system, altering sensory function (hallucinations, delusions, delirium or confusion), motor function...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07K1/113
Inventor 邵越水林燕芳
Owner 杭州博林生物技术有限公司
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