Preparation method for artificial antigen of propoxyphene

A technology of artificial antigen and artificial hapten, applied in the field of preparation of propoxyphenol artificial antigen, can solve the problems of expensive instruments, time-consuming detection and inability to achieve, and achieve the effect of advanced synthesis technology and strong specificity

Active Publication Date: 2015-06-17
杭州博林生物技术有限公司
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Problems solved by technology

[0005] At present, the detection of propoxyphene mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple (MS), etc., but there are expensive instruments, time-consuming detection, and professional skills Operation by personnel cannot meet the fast and accurate requirements of modern detection

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  • Preparation method for artificial antigen of propoxyphene
  • Preparation method for artificial antigen of propoxyphene
  • Preparation method for artificial antigen of propoxyphene

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Embodiment 1

[0031] (1) Preparation of artificial hapten:

[0032] (a) Weigh 1.13g of chirald (4mmol) into a 50ml round bottom flask, add 30ml of 3A molecular sieve-dried pyridine to dissolve, then add 4.56g of glutaric anhydride (40mmol), heat the oil bath to 100°C and stir the reaction 72 hours; After the reaction was finished, the solvent was evaporated to dryness under reduced pressure, and the obtained residue was gradually dissolved with 150ml of aqueous sodium hydroxide solution with pH=11, transferred to a 250ml separating funnel, adjusted to pH=9 with saturated aqueous sodium hydroxide solution, and the aqueous phase Wash with about 150ml of ethyl acetate for 3 times, then adjust the pH to 1 with 6N hydrochloric acid, at this time a tan insoluble matter is produced, add 200ml of dichloromethane to extract 4 times, combine the organic phases, dry over anhydrous sodium sulfate, filter, The solvent was evaporated to dryness under reduced pressure to obtain a tan sticky solid, which w...

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Abstract

The invention provides a preparation method for an artificial antigen of propoxyphene. The preparation method comprises the following steps of: (1) preparing and detecting a hapten: taking chirald as a raw material to generate a condensation reaction with glutaric anhydride, and introducing carboxyl on a nitrogen site to obtain the hapten containing the carboxyl; (2) preparing and detecting the artificial antigen: combining the hapten with bovine serum albumin (BSA) by utilizing a carbodiimide method, so as to prepare the artificial antigen of the propoxyphene, namely propoxyphene-bovine serum albumin. According to the preparation method, the prepared artificial antigen of the propoxyphene can be subjected to animal immunization to obtain corresponding propoxyphene antibodies and can be used for researching various propoxyphene immunoassay methods, and a relatively convenient, fast and accurate way is provided for the detection of the propoxyphene.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of propoxyphene artificial antigen. Background technique [0002] Propoxyphene, also known as dextropropoxyphene, is one of the commonly used opioid painkillers for the treatment of mild to moderate pain. Compared with other analgesics, dextropropoxyphene has a faster onset of action features. Its structural formula is: [0003] [0004] Taking large doses of propoxyphene can cause dysphagia, visual hallucinations and toxic psychosis, which may cause respiratory depression. Acute overdose can produce multisystem symptoms, such as convulsions, hallucinations, confusion, and even coma, respiratory depression, respiratory arrest, tachycardia, high or low blood pressure, cardiac conduction disturbance, and even cardiac arrest. According to a report by the FDA, the mortality rate of 222 cases was 8%, and it was suggested that respira...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765
Inventor 邵越水徐建
Owner 杭州博林生物技术有限公司
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