Preparation method of artificial antigen of ketamine

A technology of artificial antigen and artificial hapten, which is applied in the field of preparation of ketamine artificial antigen, can solve the problems of time-consuming detection, expensive equipment, and unreachability, and achieve the effects of high specificity, high yield, and high purity

Inactive Publication Date: 2015-04-29
ABON BIOPHARM HANGZHOU
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Problems solved by technology

[0005] At present, the detection of ketamine mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple (MS), etc.,...

Method used

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  • Preparation method of artificial antigen of ketamine
  • Preparation method of artificial antigen of ketamine
  • Preparation method of artificial antigen of ketamine

Examples

Experimental program
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Embodiment 1

[0029] (1) Preparation of artificial hapten:

[0030] (a) Weigh 200mg of ketamine hydrochloride into a 50ml Erlenmeyer flask, add 10mL of purified water to fully dissolve, then add 6 drops of ammonia water dropwise, a white turbidity will appear, extract with 3×10ml of dichloromethane, combine the organic phases, anhydrous Dry over magnesium sulfate, filter in a 50ml single-necked round-bottom flask, and turn dry to obtain 166mg of white product I. TLC: The chromatographic solution is ethyl acetate:petroleum ether=1:1 (v / v), the product R f =0.8.

[0031] (b) Add 10ml of anhydrous acetone solution, the product I dissolves, add 300mg of anhydrous potassium carbonate, 363ul ethyl bromoacetate and heat to 70-75°C, reflux for 23 hours; stop the reaction, cool to room temperature, filter the reaction solution into In a single-necked round bottom flask, the solvent was evaporated to dryness under reduced pressure to obtain 310 mg of a yellow oily liquid II. TLC: The chromatographi...

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Abstract

The invention provides a preparation method of an artificial antigen of ketamine. The preparation method comprises the following steps: (1) preparing and detecting a hapten, namely reacting the hapten containing carboxyl from ketamine hydrochloride and ethyl bromoacetate by virtue of dissociation, arm linkage and hydrolysis; (2) preparing and detecting the artificial antigen, namely combining the hapten with bovine gamma globulin (BGG) by virtue of a carbodiimide method so as to prepare the artificial antigen of the ketamine, namely ketamine-bovine gamma globulin. The prepared artificial antigen of the ketamine can be utilized for preparing corresponding ketamine antibodies by virtue of animal immunization and can be applied for researches of various ketamine immunoassay methods, and a convenient, rapid and accurate way is provided for the detection of the ketamine.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for preparing an artificial antigen of ketamine. Background technique [0002] Ketamine (KET) is a derivative of phencyclidine, which belongs to non-opiate anesthesia drugs. It is clinically used as an surgical anesthetic or anesthesia inducer, and has a certain potential for mental dependence. Developed by Professor Craig of Wayne State University. It was developed by Parker-Davis Pharmaceuticals in 1962 as a safer anesthetic to replace phencyclidine (PCP), which had severe side effects. In addition to anesthesia, ketamine also has strong analgesic effects. It is still widely used until now. At present, this drug is also widely used by veterinarians and used as an anesthetic in developing countries. Its structural formula is: [0003] [0004] Ketamine can produce a state of dissociative anesthesia, characterized by catalepsy, light sedation, amne...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/107
CPCC07K14/4717C07K19/00
Inventor 董媛媛韩洁茹
Owner ABON BIOPHARM HANGZHOU
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