Paenibacillus peoriae and application thereof in prevention and treatment of bacterial soft rot of cucumbers

A technology of bacillus and bacterial agent, applied in the field of Paenibacillus peirui and its application in the prevention and treatment of cucumber bacterial soft rot, which can solve the problems of food safety, environmental pollution, and fast transmission speed

Active Publication Date: 2021-05-07
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the onset of cucumber bacterial soft rot, it spreads quickly and has a wide range of damage. At present, chemical agents cannot effectively control the disease, and it is easy to cause environmental pollution and food safety problems. Therefore, it is particularly important to carry out research on the biological control of cucumber bacterial soft rot. important and urgent

Method used

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  • Paenibacillus peoriae and application thereof in prevention and treatment of bacterial soft rot of cucumbers
  • Paenibacillus peoriae and application thereof in prevention and treatment of bacterial soft rot of cucumbers
  • Paenibacillus peoriae and application thereof in prevention and treatment of bacterial soft rot of cucumbers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1, Isolation, Screening, Identification and Preservation of Paenibacillus russica ZF390

[0060] 1. Isolation of strains

[0061] Bacterial strains were isolated from potato rhizosphere soils in Heilongjiang, Sichuan, Hebei, Inner Mongolia and Shandong provinces, and a total of 1200 pure cultured bacterial strains were isolated.

[0062] The specific method is as follows: Weigh 10 g of potato rhizosphere soil, dissolve it in 90 mL of sterile water, and shake it at 28 °C and 180 r / min for 30 min to make it fully mixed; -2 to 10 -5 Carry out gradient dilution, draw 100 μL of the diluted suspension to coat LB solid medium plates (3 plates for each dilution), and after 2 days of constant temperature cultivation at 28°C, pick bacterial colonies with different shapes on the LB solid medium Streak purification on the plate, and then numbered and saved.

[0063] 2. Strain screening

[0064] 1. In vitro screening

[0065] Test bacteria: 1200 bacterial strains isolat...

Embodiment 2

[0100] Embodiment 2, Paenibacillus russia ZF390 antibacterial spectrum determination of Peirui

[0101] 1. Determination of fungal inhibition spectrum

[0102] The inhibitory rate of Paenibacillus peirui ZF390 to various pathogenic fungi was detected by plate confrontation method.

[0103] The pathogenic fungi are: Corynespora multimata, Fusarium oxysporum, capsicum anthracnose, Phytophthora capsici, Puccinia solani, Bispora watermelon, Botrytis cinerea or Rhizoctonia solani.

[0104] The specific method is as follows: Pick a single colony of Paenibacillus peirui ZF390 and inoculate it in LB liquid medium, and culture it with shaking at 28°C and 180r / min until the concentration is 1×10 8 cfu / mL, which is the ZF390 bacterial suspension; inoculate a pathogenic fungus cake with a diameter of 5 mm in the center of the PDA medium plate, and at the same time inoculate 5 μL of the ZF390 bacterial suspension at a place 3.5 cm away from the central plate (use LB liquid medium as the Z...

Embodiment 3

[0133] Embodiment 3, the enzyme activity assay of Paenibacillus russica ZF390

[0134] 1. Pick a single colony of Paenibacillus peirui ZF390 and inoculate it in LB liquid medium, and culture it with shaking at 28°C and 180r / min until the bacterial concentration is 1×10 8 cfu / mL is the ZF390 bacterial suspension.

[0135] 2. Take 5 μL of ZF390 bacterial suspension and inoculate it in the center of the CMC medium plate, and incubate at 28°C for 24 hours.

[0136] CMC medium: 0.1 g of magnesium sulfate, 1 g of ammonium sulfate, 10× phosphate buffer (70 g of dipotassium hydrogen phosphate, 20 g of potassium dihydrogen phosphate, 1000 mL of distilled water) 100 mL, 5 g of yeast extract, 2 mL of glycerol, sodium carboxymethyl cellulose 1 g, 8 g of agar (bacto agar), 900 mL of distilled water.

[0137] 3. Take 5 μL of ZF390 bacterial suspension and inoculate it in the center of the protease medium plate, incubate at 28°C for 24 hours, and then measure the diameter of the transparen...

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Abstract

The invention discloses Paenibacillus peoriae and application thereof in prevention and treatment of bacterial soft rot of cucumbers. The invention provides Paenibacillus peoriae ZF390 with the preservation number of CGMCC No. 20322. The invention also protects a fermentation product of the Paenibacillus peoriae ZF390. The invention also protects a microbial agent containing the Paenibacillus peoriae ZF390. The invention also protects application of the Paenibacillus peoriae ZF390 or the fermentation product or the microbial agent in inhibition of pathogenic bacteria. The invention also protects application of the Paenibacillus peoriae ZF390 or the fermentation product or the microbial agent in prevention and treatment of plant diseases caused by the pathogenic bacteria. The Paenibacillus peoriae ZF390 provided by the invention can be used for inhibiting the plant pathogenic bacteria, so that the plant diseases caused by the plant pathogenic bacteria are prevented and controlled.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Paenibacillus peirui and its application in the prevention and treatment of cucumber bacterial soft rot. Background technique [0002] Cucumber (Cucumis sativas L.) is an important fruit-type vegetable and the main cultivated species of facility vegetables. In recent years, with the continuous expansion of facility cucumber planting scale, the occurrence of bacterial diseases has become more and more common, which has caused serious economic losses to my country's cucumber industry. Pectobacterium can cause soft rot of various plants such as cucumber, potato, Chinese cabbage, calla lily, konjac, tulip, etc., and has a very wide range of hosts. Economically speaking, soft rot is one of the top ten most destructive bacterial diseases affecting plants worldwide. After the onset of cucumber bacterial soft rot, it spreads quickly and has a wide range of damage. At present, che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P1/00C12R1/01
CPCC12N1/20A01N63/25
Inventor 李宝聚李磊赵昱榕谢学文石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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