Primer group for detecting field pectobacterium carotovorum subsp and kit and method for extracting and detecting DNA of pectobacterium carotovorum subsp

A technology of pectin bacillus and soft rot pectin, which is applied in the field of gene detection and diagnosis and nucleic acid extraction, can solve the problems of lack of rapid extraction of nucleic acid of soil-borne pathogenic bacteria, and the assumption and verification of integrated detection methods, so as to avoid false positive problems, Simple operation and easy operation effect
CN110218804AActive Publication Date: 2019-09-10HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2019-09-10

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Abstract

The invention discloses a primer group for detecting field pectobacterium carotovorum subsp and a kit and method for extracting and detecting DNA of the pectobacterium carotovorum subsp. The kit combines a sample nucleic acid rapid extracting technology and a loop-mediated isothermal amplification technology to establish a bacterial soft rot pathogenic bacterium product based on a soft rot pathogenic bacterium specific target single-base difference phenomenon. The detection method has the advantages that soft rot pathogenic bacteria, plant tissue and soil samples can be rapidly detected separately, nucleic acid extraction can be completed within 30 seconds, detection only needs 30-40 minutes, detection is rapid and accurate, no expensive instrument is needed, the cost is low, and the detection method can be directly used for real-time and on-site detection in the field, which facilitates dynamic monitoring of the soft rot pathogenic bacteria in soil, facilitates the development of popular prediction and early warning work of the soft rot, and facilitates early disease prevention and treatment.
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Description

technical field

[0001] The invention relates to the fields of nucleic acid extraction and gene detection and diagnosis, in particular to a primer set for detecting field Pectinobacillus soft rot subspecies carotene, a rapid detection kit and a method thereof. Background technique

[0002] At this stage, with the development of protected areas, continuous cultivation of high value-added crops, straw returning to the field, and promotion of no-tillage techniques, a large number of pathogenic bacteria accumulate in the soil, causing soil-borne diseases to occur. Soil-borne diseases have the characteristics of various types, wide distribution, rapid spread, great harm, and difficult control. Pathogens can overwinter in the soil and continue to attack crops in the coming year, and this cycle makes the disease more serious. In general, soil-borne diseases damage the roots and stems of plants. In the early stage of crop growth, the roots and stems of seedlings can rot and die; Us...

Claims

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