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Bacillus subtilis M3 and application thereof

A technology for the production of Bacillus subtilis and strains, applied in the fields of application, bacteria, and fungicides, can solve environmental and human health hazards, accelerate the research and development of biopesticides and application research, and achieve good comprehensive properties, good growth-promoting effects, Ease of industrial production

Inactive Publication Date: 2015-04-22
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 4. Konjac bacterial soft rot
[0014] At present, the extensive use of chemical pesticides to prevent and control plant diseases has caused harm to the environment and human health, which has accelerated the development and application of biological pesticides

Method used

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  • Bacillus subtilis M3 and application thereof
  • Bacillus subtilis M3 and application thereof
  • Bacillus subtilis M3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of biological preparations (the following are weight percentages)

[0024] The production strain is Bacillus subtilis M3,

[0025] Deposit unit: General Microbiology Center of China Microbiological Culture Collection Management Committee; preservation date: July 11, 2013; preservation number: CGMCC NO.7911.

[0026] Transfer Bacillus subtilis (Bacillus subtilis) M3 to the slant medium of the test tube. The formula of the slant medium of the test tube is: beef extract 0.3%, yeast extract 0.1%, peptone 0.5%, glucose 1.0%, agar 1.7%, and the water content is 96.1%-96.4%, pH: 7.0. Incubate at 28°C for 2 days to obtain test tube species.

[0027] The formula of the liquid fermentation medium is: soybean powder 2.0%, starch 1.0%, glucose 0.30%, KH 2 PO 4 0.1%, peptone 0.2% (NH 4 ) 2 SO 40.05%, MgSO 4 ·7H 2 O0.03%, CaCO 3 0.1%, peanut oil 0.1%, defoamer 0.01%, water content 96.11%, pH7.5;

[0028] Inoculate the test tube seed into a 500mL tria...

Embodiment 2

[0039] Embodiment 2: Biocontrol fungicide M3 is to the drug effect test of Chinese cabbage clubroot

[0040] 1. Test materials

[0041] The experiment was arranged in the greenhouse of Yunnan Agricultural University. Field experiments were conducted in Wangwuying Village, Tonghai County, Yuxi City, Yunnan Province, and Chahe Village, Jinshan Town, Lufeng County, Chuxiong Prefecture, a total of three plots with severe clubroot occurred. The previous crops in one of the fields in Wangwuying and Chahe Village were non-cruciferous crops , another crop in Chahe Village is cabbage. Chinese cabbage varieties for testing: Qingdao 83-1 (Qingdao International Seedling Co., Ltd.), Chunxi Bawang No. 1 (Kunming Chunxi Agricultural Science and Technology Development Co., Ltd.), Chengza No. 5 (Yunnan Yunhong Vegetable and Flower Seedling Research Institute).

[0042] Inoculation method of pathogenic bacteria: Take 15g of fresh weight homogenate for each pathogen, prepare 100mL of bacterial...

Embodiment 3

[0067] Embodiment 3: Biocontrol agent M3 is to tomato scab indoor bacteriostasis, field efficacy test

[0068] The greenhouse pot experiment was carried out in the greenhouse of Yunnan Agricultural University in 2012. The tomato variety grown in the experiment was Rutgus. Two methods of biocontrol bacteria inoculation were used in this experiment. One of them is spray inoculation. Sow tomato seedlings in pots first. When the tomato grows 4-6 true leaves, the prepared M3 fermented liquid (OD 590 =0.25) 10mL and pathogenic bacteria liquid (OD 590 =0.25) 10mL, mixed and sprayed on tomato leaves. The other is the soaking method. That is, before sowing tomatoes with OD 590 = 0.25 M3 fermentation broth to soak tomato seeds, and then use carboxymethyl cellulose for seed coating. Then placed in a constant temperature shaker at 28°C for 2h. Then sow the tomatoes in pots. When the tomato grows 4-6 true leaves, the prepared pathogenic bacteria liquid (OD 590 =0.25) 10mL was sp...

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Abstract

The invention relates to bacillus subtilis M3 and an application thereof, and belongs to the technical field of biological pesticides. The produced strain is the bacillus subtilis M3; the preservation unit is the China General Microbiological Culture Collection Center; the address is Zhongguancun, Beijing, China; the preservation date is July 11, 2013; the preservation number is CGMCC NO.7911. The application of the bacillus subtilis M3 as a preparation for preventing and controlling Chinese cabbage clubroot, tomato scab, tomato root knot nematode diseases and konjak bacterial soft rot is also disclosed. The bacillus subtilis M3 disclosed by the invention achieves an obvious and stable prevention and control effect on the diseases, can be used for preparing the preparation for preventing and controlling the Chinese cabbage clubroot, the tomato scab, the tomato root knot nematode diseases and the konjak bacterial soft rot and has the advantages of high efficiency, nontoxicity, safety, no residue, bacteria activation, disease control, better growth promotion effect, better comprehensive character and easiness for industrialized production.

Description

Technical field: [0001] The invention relates to a bacillus subtilis M3 and its application, belonging to the technical field of biological pesticides. Background technique: [0002] 1. Clubroot of cruciferous crops [0003] Cruciferous crop clubroot is a worldwide disease caused by Plasmodiophora brassicae Woron. The disease mainly harms the roots of plants, and is usually susceptible to both the seedling stage and the adult plant stage, and is mainly susceptible to the seedling stage. Usually the seedlings are damaged, and in severe cases, the seedlings die, and the yield is severely reduced, or even the harvest is not harvested; once the field is polluted by clubroot, the soil will be infected for a long time, which seriously threatens the sustainable production of cruciferous crops. In recent years, the incidence of P. brassicae has become more and more serious all over the world, especially in Asia, Northern Europe, Australia, North America and Japan. Clubroot has bec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00A01P5/00C12R1/125
Inventor 姬广海魏兰芳周丽洪何月秋方敦煌
Owner YUNNAN AGRICULTURAL UNIVERSITY
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