32 results about "Hypopygiopsis tumrasvini" patented technology

The invention discloses a cultivation method capable of controlling tomato root knot nematode. The cultivation method includes that innovations are made on double-concave-structure cultivation ridges, a sunlight greenhouse tomato special planting mode is set, organic combining of biological trapping and biological fumigating is realized, and the objective of controlling damage of tomato root knot nematode is achieved. No chemical pesticide is used in the cultivation method, so that tomato root knot nematode can be effectively prevented and controlled, root knot index of tomatoes is lowered greatly, yield of the tomatoes is increased, and the cultivation method is low in energy consumption and meets the low-carbon concept.

The invention discloses a cultivation method for preventing tomato root-knot nematode worms. Due to the facts that concave-concave type structure cultivation ridges are innovated, and a solar greenhouse tomato special type planting mode is set, organic combination among biological avoidance, biological trapping and biological fumigant is achieved, and the purpose of control over the hazard of the root-knot nematode worms is achieved. According to the cultivation method, no chemical pesticide is used, the root-knot nematode worms can be effectively prevented, the root-knot index of tomatoes is greatly reduced, the yield of the tomatoes is improved, energy consumption is low, and the low-carbon concept is met.

The invention relates to bacillus subtilis M3 and an application thereof, and belongs to the technical field of biological pesticides. The produced strain is the bacillus subtilis M3; the preservation unit is the China General Microbiological Culture Collection Center; the address is Zhongguancun, Beijing, China; the preservation date is July 11, 2013; the preservation number is CGMCC NO.7911. The application of the bacillus subtilis M3 as a preparation for preventing and controlling Chinese cabbage clubroot, tomato scab, tomato root knot nematode diseases and konjak bacterial soft rot is also disclosed. The bacillus subtilis M3 disclosed by the invention achieves an obvious and stable prevention and control effect on the diseases, can be used for preparing the preparation for preventing and controlling the Chinese cabbage clubroot, the tomato scab, the tomato root knot nematode diseases and the konjak bacterial soft rot and has the advantages of high efficiency, nontoxicity, safety, no residue, bacteria activation, disease control, better growth promotion effect, better comprehensive character and easiness for industrialized production.

The invention discloses an application of bacillus cereus BCJB01 in control of root-knot nematodes. A supernatant and a bacterial suspension of the bacillus cereus BCJB01 have a completely lethal effect on second instar larvae of tomato root-knot nematodes and eggs being about to hatch out the second instar larvae. One new strain for controlling the root-knot nematodes is provided, the effect of the bacillus cereus BCJB01 is not lower than that of an existing chemical agent in the market, and great progress in the aspect of control of the root-knot nematodes is achieved.

The invention discloses a strain of bacillus altitudinis and application thereof in control of tomato root-knot nematode. The bacillus altitudinis strain YT2002 has been deposited in the China GeneralMicrobiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15213; experimental results show that the bacillus altitudinis strain YT2002 biological agenthas a good effect on control of tomato root-knot nematode, with the control effect of 63.03% after 14 days after the last application; from growth of the tomato, the bacillus altitudinis strain YT2002 has an obvious effect on promoting tomato growth, and is safe and reliable without risk of damage.

The invention discloses a root-knot nematode biocontrol bacterium, an antibiological inoculant and application of the biocontrol bacterium and the antibiological inoculant. The biocontrol bacterium is a Providencia rettgeri strain UN06, and the preservation serial number of the biocontrol bacterium is CGMCC No. 10246. Fermentation liquor obtained through fermentation of the Providencia rettgeri strain UN06, adsorbing carriers such as mushroom dreg and grass carbon, and nutrient substance urea required by bacterium growth are evenly mixed to be granulated to form UN06 granules. The control efficiencies of 100 kilograms of the applied UN06 granules in each mu to tomato root-knot eelworms, cucumber root-knot nematodes and tobacco root-knot nematodes are 90.17 percent, 88.66 percent and 85.98 percent. The root-knot nematode antibiological inoculant has the advantages of being low in cost, high in control efficiency, free of residual and safe to people and livestock, and is an efficient biopesticide for preventing and treating root knot nematode diseases.

The invention provides an application of a T11-W bacterial strain in agriculture, and particularly relates to the application of the T11-W bacterial strain in the prevention and cure of soil-borne nematodiasis, especially root-knot nematode diseases of vegetables. The T11-W bacterial strain can be used for effectively preventing and controlling the root-knot nematode diseases of tomatoes. Whether the T11-W bacterial strain is subjected to soil-mixed treatment or root-irrigation treatment, when the effective spore amount each pot is 2*10<7>, the prevention and control effect of the T11-W bacterial strain is above 77%. For the root-irrigation treatment, when the effective spore amount each pot is 2*10<8>, the prevention and control effect of the T11-W bacterial strain reaches up to 90.37%.

The invention discloses a Siamese bacillus and an application of the same in controlling tomato root-knot nematode disease. The strain is identified as Bacillus siamensis LK0006 (CGMCC No. 15215). Theresults showed that the inhibition rate of nematodes is 90.35% when the fermentation broth is diluted 25 times and treated for 48 hours in vitro. The hatching of eggs could be inhibited by 25, 50, 100 times dilution of fermentation broth. Bacillus siamensis LK0006 wettable powder had a significant control effect on tomato root-knot nematode disease (75.02%) under pot culture condition, and tomatohad strong growth and no pesticide harm, so it had a good prospect in biocontrol of tomato root-knot nematode disease.

The invention discloses a control method for a greenhouse tomato root knot nematode disease. The morbidity of the greenhouse tomato root knot nematode disease can be greatly lowered, the yield of greenhouse tomatoes is increased, and the quality of the greenhouse tomatoes is improved. The method comprises the following steps of crop rotation implementation, seed treatment, soilless culture of nematode-free strong seedlings, grafting cultivation, soil disinfection, biological control and chemical control. Accordingly, the means of early prevention is adopted, it is clarified that 40% isofenphos methyl E.C. or nematode repellent powder is used for conducting biological control on soil, and meanwhile biological control and chemical control are combined. The experiment shows that by means of soil ecological control, the tomato root knot nematode disease can be effectively prevented, the control efficiency reaches 90% or above, the average morbidity of the tomato root knot nematode disease is significantly lowered, the tomato yield is increased, the tomato quality is significantly improved, and the good economic benefit is achieved.

The invention relates to a technology for preventing and curing knot nematode of tomatoes by using edible mushroom dregs. The knot nematode does great harm to greenhouse tomatoes, thereby being a big obstacle for producing the greenhouse tomatoes. The technical scheme adopted by the invention is as follows: applying additional materials containing needle mushroom dregs when performing ridging and laminating on the tomatoes; and closing a greenhouse for 15 days at a high temperature after performing broad irrigation on the green tomatoes. The temperature in the greenhouse is kept at 65 DEG C, so as to realize the effect of closing the greenhouse at a high temperature. The area ratio of the needle mushroom dregs to the land area is 20m<3> / 667m<2>. The technology provided by the invention has the following beneficial effects: the harm of the knot nematode can be greatly reduced, the applied material is green and environmentally friendly, has no pollution and meets the relative requirements on sustainable development; and application of the additional mushroom dregs in soil and the closing of the greenhouse at a high temperature in summer can obviously raise the temperature of the soil and the green house, so as to kill germs in the soil. According to the invention, the high temperature is raised when the mushroom dregs ferment in the closing of the greenhouse at a high temperature, and 45% of phoxim emulsifiable missible oil is added, so that the knot nematode can be effectively prevented and cured under the combined action of killing with poison and high-temperature limitation.

The invention discloses exiguobacterium aurantiacum and application thereof in prevention and control of tomato root-knot nematode. The strain is an exiguobacterium aurantiacum strain 8220605, is obtained by being separated from the lake mud of the Nansi Lake in Jining City of Shandong province, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 26, 2017,and has a preservation number of CGMCC No. 14676. Test results prove that the exiguobacterium aurantiacum strain 8220605 biological control preparation is remarkable in prevention and control effect of the tomato root-knot nematode, and the prevention and control effect reaches 74.25%; furthermore, after the exiguobacterium aurantiacum is used, tomatoes grow well and are protected from pesticide hazard.

The invention discloses a preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and an application thereof. The preparation for prevention and treatment of tobacco and tomato meloidogyne spp. has an active ingredient of a fermentation liquid of a strain of bacillus amyloliquefaciens YN2010-KC CGMCC No.5722. The preparation not only is a microbial fertilizer which can significantly promote the growth of plants, but also is a biological pesticide and has the average relative control efficiency on tobacco meloidogyne spp. disease of up to 78.32%; the preparation has the control efficiency on the tomato meloidogyne spp. disease of up to 74.50% or more, and has remarkable prevention and treatment effect. The preparation has low production cost, an application method is simple and easy to implement, and the preparation can be used for direct seed dressing and root irrigation, has no toxicity, no pollution and no residue, and is safe to human and livestock.

The invention belongs to the technical field of combination of pesticides and specifically relates to a nematicidalcompound pesticide comprising main effective ingredients of tioxazafen and imicyafos at weight part ratio of 1:20-15:1. In the nematicidalcompound pesticide, the active ingredients has an obvious synergistic interaction on preventing and controlling nematodes within the weight ratio; the nematicidalcompound pesticide is especially suitable for preventing and controlling wheat root-knot nematodes and cyst nematodes, citrus root-knot nematodes, cucumber root-knot nematodes, tomato root-knot nematodes, pepper root-knot nematodes, peanut root-knot nematodes and soybeancyst nematodes.

The invention discloses a microbial composite inoculum and applications thereof, belonging to the technical field of microbial pesticides. The microbial composite inoculum provided by the invention is prepared by a method comprising the following steps: selecting microbial bacterial strain verticillium chilmaydosporium ZK7 with the preservation number CGMCC No. 0418 and bacillus nematocidus B16 with the preservation number CGMCC No.1128 which are preserved in the common microbial center of China committee for culture collection of microorganisms; respectively preparing a ZK7 spore suspension and a B16 bacteria suspension according to the conventional method; and then mixing according to the mass ratio of (0.5-2)-(0.2-1), thus a liquid inoculum is obtained. The microbial composite inoculum prepared by the invention is prepared for the applications of a preparation for preventing and treating plant nematode. The microbial composite inoculum provided by the invention has the advantages of high efficiency, low toxicity and residue free, and achieves room temperature prevention of more than 90% for tomato root knot nematode disease.

The invention relates to the technical field of agricultural crop disease prevention and treatment, and in particular, relates to a composite microbial agent for prevention and treatment of tomato root knot nematode disease and an application thereof; a tomato symbiotic AM fungus (glomus versiforme) and plant growth-promoting rhizobacteria PGPR (bacillus subtilis) are used together, and the synergistic effect of the two microorganisms is utilized; compared with a prevention and treatment effect of a general single inoculum or rhzomorph, the prevention and treatment rate reaches 60-70% and is higher than that of the single inoculum or rhzomorph. At the same time, tomato plants applied with the composite microbial agent have developed root systems and especially have more fibrous root systems, and the absorption area of tomato is effectively increased.

The present invention relates to a novel nematode killing composition, wherein the active components of the novel nematode killing composition comprise tioxazafen and iprodione, and a weight of tioxazafen to iprodione is 1:30-30:1. According to the present invention, the novel nematode killing composition provides significant synergistic effect on the prevention and control of meloidogyne diseases of a variety of crops within the compounding range of the active components, is suitable for prevention and control of wheat meloidogyne, cyst nematode, citrus meloidogyne, cucumber meloidogyne, tomato meloidogyne, hot pepper meloidogyne, peanut meloidogyne, soybean cyst nematode and other nematodes, and has characteristics of good prevention and control effect, long lasting effect, and easy promotion and application.

The invention discloses application of gibberella intermedia QJP2 fermentation extract to prevention and control of plant inset pests and pathogenic nematodes and belongs to the field of microbial pesticide. A strain is preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number is CGMCC No. 13199. The gibberella intermedia QJP2 fermentation extract is obtained by fermenting the strain through a shaking table and extracting with ethyl acetate and has relatively insecticidal activity on the plant inset pests including Plutela xylostella and the like; the fatality rate reaches 85.6+ / -1.3 percent when the concentration of the gibberella intermedia QJP2 fermentation extract is 1.0mg / mL; moreover, the gibberella intermedia QJP2 fermentation extract also has relatively good inhibition activity on the root knot nematode disease of tomatoes, and the fatality rates of 50-time and 100-time diluents of the gibberella intermedia QJP2 fermentation extract on the root knot nematode disease of the tomatoes reach 95 percent and 81 percent respectively. Therefore, the gibberella intermedia QJP2 fermentation extract can be used for preparing the microbial pesticide for preventing and controlling the plant inset pests and the pathogenic nematodes; compared with chemically synthetized pesticide, the microbial pesticide also has the advantages of environmental friendliness, low toxin to human and livestock and the like and has a relatively good application prospect.

The invention discloses a method for doubly preventing tomato root-knot nematode by combining efficient RNA (Ribonucleic Acid) with Bt gene, and belongs to the field of preventing of plant diseases and pests. The method comprises the following steps of: using transgenic tomato which is transferred with an RNA interference vector pCAGC1341-TGPM01RNAi as a host plant transfer explant; transferring a vector of expression Bt-Cry6A crystal protein to obtain a transgenic tomato variety capable of doubly preventing root-knot nematode. The invention further provides an optimization method for trans-gene. The gene synthesized in the invention is obtained by optimizing codon. The optimized artificial gene is coded with Bt-Cry6A crystal protein, and the tomato variety which is transferred with the gene has a good root-knot nematode preventing effect compared with the tomato variety which is transferred with the single gene.

The invention relates to a nematicidal compound which comprises the following active ingredients: a compound in the formula I and iprodione, wherein the weight ratio of the compound in the formula I and the iprodione is 30: 1-1: 20, and preferably, the weight ratio is 10: 1-5: 1. The active ingredients of the nematicidal compound has an obvious synergistic effect in prevention of various crop rootknot nematode diseases within a compound scope and is suitable for preventing various nematodes such as a wheat root knot nematode, a wheat cyst nematode, a citrus root knot nematode, a cucumber rootknot nematode, a tomato root knot nematode, a chili root knot nematode, a peanut root knot nematode, a soybean root knot nematode and the like. The nematicidal compound is good in prevention effect and long in lasting period, so that the nematicidal compound is worthy of promotion and application.

The invention relates to a novel nematicidal compound which comprises the following active ingredients: a compound in the formula I and tioxazafen, wherein the weight ratio of the compound in the formula I and the tioxazafen is 1: 30-30: 1, and preferably, the weight ratio is 1: 10-10: 1. The active ingredients of the nematicidal compound has an obvious synergistic effect in prevention of variouscrop root knot nematode diseases within a compound scope and is suitable for preventing various nematodes such as a wheat root knot nematode, a wheat cyst nematode, a citrus root knot nematode, a cucumber root knot nematode, a tomato root knot nematode, a chili root knot nematode, a peanut root knot nematode, a soybean root knot nematode and the like. The nematicidal compound is good in preventioneffect and long in lasting period, so that the nematicidal compound is worthy of promotion and application.

The invention relates to a novel nematode killing composition. The nematode killing composition comprises the following active ingredients: a compound as shown as formula 1, and fluensulfone at a weight ratio ranging from 1:40 to 30:1, further perferably 1:15 to 5:1. When the active ingredients are within the proportional range, the nematode killing composition shows an obvious synergetic effect on prevention and control of various crop root knot nematode diseases, and is particularly applicable to the prevention and control of various nematodes such as wheat root knot nematodes, cyst nematodes, citrus root knot nematodes, cucumber root knot nematodes, tomato root knot nematodes, capsicum root knot nematodes, peanut root knot nematodes and soybean cyst nematodes; the nematode killing composition has a good prevention and control effect, and is long in lasting period and worthy of popularization and application.

The invention provides a method of prevention and treatment of tomato root knot nematode disease by castor bean meal. The method is used for prevention and treatment of the tomato root knot nematode disease and includes the steps of crushing and sieving the castor bean meal and mixing the castor bean meal with soil for culture of tomato roots. In the invention, culture soil for the tomato roots is treated with the crushed castor bean meal, so that prevention and treatment of the tomato root knot nematode disease are effectively improved. The method has high environmental compatibility and high environmental protection property, and is low in labor intensity.

The invention provides an application of a T11-W bacterial strain in agriculture, and particularly relates to the application of the T11-W bacterial strain in the prevention and cure of soil-borne nematodiasis, especially root-knot nematode diseases of vegetables. The T11-W bacterial strain can be used for effectively preventing and controlling the root-knot nematode diseases of tomatoes. Whether the T11-W bacterial strain is subjected to soil-mixed treatment or root-irrigation treatment, when the effective spore amount each pot is 2*10<7>, the prevention and control effect of the T11-W bacterial strain is above 77%. For the root-irrigation treatment, when the effective spore amount each pot is 2*10<8>, the prevention and control effect of the T11-W bacterial strain reaches up to 90.37%.

The invention discloses an application of bacillus cereus BCJB01 in control of root-knot nematodes. A supernatant and a bacterial suspension of the bacillus cereus BCJB01 have a completely lethal effect on second instar larvae of tomato root-knot nematodes and eggs being about to hatch out the second instar larvae. One new strain for controlling the root-knot nematodes is provided, the effect of the bacillus cereus BCJB01 is not lower than that of an existing chemical agent in the market, and great progress in the aspect of control of the root-knot nematodes is achieved.

The invention relates to a novel nematicidal composition containing cartap. The nematicidal composition comprises the active components of a compound of a formula 1 and cartap, wherein the weight ratio of the compound of the formula 1 to the cartap is (5-50):(3-45), and furthermore the weight ratio is preferably (20-40):(8-35). Through the nematicidal composition containing the active components in the ratio range, an obvious synergistic effect on control of root-knot nematode of multiple crops is achieved, and the nematicidal composition is particularly suitable for control of root-knot nematode and cyst nematode of wheat, citrus root-knot nematode, cucumber root-knot nematode, tomato root-knot nematode, capsicum annuum root-knot nematode, peanut root-knot nematode, soybean cyst nematodeand other nematode. The nematicidal composition has a good control effect and long duration of efficacy, and is worthy of promotion and application.

The invention relates to a nematicide composition containing fludioxonil. Active ingredients of the nematicide composition comprise a compound as shown in formula 1 and the fludioxonil, and the weightratio of the compound as shown in formula 1 to the fludioxonil is 40: 1-1: 1, and is 15: 1-5: 1 preferably. Prevention and control of the active ingredients of the nematicide composition to various crop root knot nematode diseases in the ratio range have obvious synergistic interaction effects, and the nematicide composition containing the fludioxonil is particularly suitable for various nematodes such as wheat root-knot nematode and heterodera glycines Ichinohe, orange root-knot nematode, cucumber root-knot nematode, tomato root-knot nematode, chili root-knot nematode, peanut root-knot nematode and soybean heterodera glycines Ichinohe. The nematicide composition containing the fludioxonil is good in prevention and control effect, long in lasting period and worthy of being popularized andused.

The invention discloses a cultivation method for preventing tomato root-knot nematode worms. Due to the facts that concave-concave type structure cultivation ridges are innovated, and a solar greenhouse tomato special type planting mode is set, organic combination among biological avoidance, biological trapping and biological fumigant is achieved, and the purpose of control over the hazard of the root-knot nematode worms is achieved. According to the cultivation method, no chemical pesticide is used, the root-knot nematode worms can be effectively prevented, the root-knot index of tomatoes is greatly reduced, the yield of the tomatoes is improved, energy consumption is low, and the low-carbon concept is met.

The invention discloses a root-knot nematode biocontrol bacterium, a biocontrol bacterium agent and applications thereof. The biocontrol bacterium is Providencia rettgeri (Providencia rettgeri) strain UN06, and the preservation number is CGMCC No.10246. The present invention utilizes the fermented liquid obtained by the fermentation of the Providencia rettgeri UN06 strain to mix evenly with adsorption carriers such as bacterial residue and peat and urea, a nutrient substance required for bacterial growth, and then granulate to prepare the UN06 granule. The control effects of applying 100 kg of UN06 granules per mu on tomato root-knot nematode, cucumber root-knot nematode and tobacco root-knot nematode were 90.17%, 88.66% and 85.98%, respectively. The root-knot nematode biological control bacterial agent of the invention has the characteristics of low cost, high control effect, no residue, and safety to humans and animals, and is a high-efficiency biological pesticide for preventing and treating root-knot nematode.

The invention relates to an Aspergillus niger strain ANUV90 obtained through induced mutation, which has high and stable resistance for carbendazim. Compared with the resistance for carbendazim of theinitial strain AN, the resistance for carbendazim of the strain is increased by1000 times. The Aspergillus niger strain ANUV90 can grow normally under the 1000 Mu g / mL treatment concentration of effective component of carbendazim. And the fermentation broth of the strain has certain nematode killing effects. No significant difference of colony morphology characteristic is detected between the strains before and after induced mutation. And the strain has a certain biological activity on the tomato root knot nematodes (Meloidogyne spp.). After analyzing the molecular mechanism of the resistanceof the mutant strain, the primary research result shows that the site directed mutation of Beta- tubulin encoding gene leads to the generation of the biological characteristic resistance of ANUV90. The bacterial strain related to the invention is stored in China General Microbiological Culture Collection Center on 30 October, 2007. And the preservation number of the strain is CGMCC No: M2234. Theclassification and nomenclature of the strain is Aspergillus niger. Due to the resistance characteristic, the strain can be mixed and applied with most common pesticides for the control and prevention of various harmful insects. The invention provides a way for solving the unstable control effect problem in the biocontrol strain field, which has great application and development prospect.