32 results about "Hypopygiopsis tumrasvini" patented technology

The invention discloses an application of bacillus cereus BCJB01 in control of root-knot nematodes. A supernatant and a bacterial suspension of the bacillus cereus BCJB01 have a completely lethal effect on second instar larvae of tomato root-knot nematodes and eggs being about to hatch out the second instar larvae. One new strain for controlling the root-knot nematodes is provided, the effect of the bacillus cereus BCJB01 is not lower than that of an existing chemical agent in the market, and great progress in the aspect of control of the root-knot nematodes is achieved.

The invention discloses a root-knot nematode biocontrol bacterium, a biocontrol bacterium agent and applications thereof. The biocontrol bacterium is Providencia rettgeri (Providencia rettgeri) strain UN06, and the preservation number is CGMCC No.10246. The present invention utilizes the fermented liquid obtained by the fermentation of the Providencia rettgeri UN06 strain to mix evenly with adsorption carriers such as bacterial residue and peat and urea, a nutrient substance required for bacterial growth, and then granulate to prepare the UN06 granule. The control effects of applying 100 kg of UN06 granules per mu on tomato root-knot nematode, cucumber root-knot nematode and tobacco root-knot nematode were 90.17%, 88.66% and 85.98%, respectively. The root-knot nematode biological control bacterial agent of the invention has the characteristics of low cost, high control effect, no residue, and safety to humans and animals, and is a high-efficiency biological pesticide for preventing and treating root-knot nematode.

The invention discloses a cultivation method capable of controlling tomato root knot nematode. The cultivation method includes that innovations are made on double-concave-structure cultivation ridges, a sunlight greenhouse tomato special planting mode is set, organic combining of biological trapping and biological fumigating is realized, and the objective of controlling damage of tomato root knot nematode is achieved. No chemical pesticide is used in the cultivation method, so that tomato root knot nematode can be effectively prevented and controlled, root knot index of tomatoes is lowered greatly, yield of the tomatoes is increased, and the cultivation method is low in energy consumption and meets the low-carbon concept.

The invention relates to an Aspergillus niger strain ANUV90 obtained through induced mutation, which has high and stable resistance for carbendazim. Compared with the resistance for carbendazim of theinitial strain AN, the resistance for carbendazim of the strain is increased by1000 times. The Aspergillus niger strain ANUV90 can grow normally under the 1000 Mu g / mL treatment concentration of effective component of carbendazim. And the fermentation broth of the strain has certain nematode killing effects. No significant difference of colony morphology characteristic is detected between the strains before and after induced mutation. And the strain has a certain biological activity on the tomato root knot nematodes (Meloidogyne spp.). After analyzing the molecular mechanism of the resistanceof the mutant strain, the primary research result shows that the site directed mutation of Beta- tubulin encoding gene leads to the generation of the biological characteristic resistance of ANUV90. The bacterial strain related to the invention is stored in China General Microbiological Culture Collection Center on 30 October, 2007. And the preservation number of the strain is CGMCC No: M2234. Theclassification and nomenclature of the strain is Aspergillus niger. Due to the resistance characteristic, the strain can be mixed and applied with most common pesticides for the control and prevention of various harmful insects. The invention provides a way for solving the unstable control effect problem in the biocontrol strain field, which has great application and development prospect.