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Parasitism effect of trichoderma T11-W on root-knot nematode egg and application of trichoderma T11-W

A technology of root-knot nematode and southern root-knot nematode, applied in application, nematicide, biocide and other directions, can solve problems such as unreported parasitic effect of vegetable root-knot nematode eggs

Active Publication Date: 2015-06-10
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that the secondary metabolites of Trichoderma harzianum Snef85 are toxic to root-knot nematode larvae (Duan Yuxi, Jin Yingying, Wang Shengjun, etc. Identification of the biocontrol strain Snef85 and the toxicity of its fermentation liquid to different types of nematodes [J]. Acta Plant Protection, 2008, 35 (2): 132-136), but the parasitic effect of Trichoderma harzianum on root-knot nematode eggs of vegetables has not been reported

Method used

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  • Parasitism effect of trichoderma T11-W on root-knot nematode egg and application of trichoderma T11-W
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  • Parasitism effect of trichoderma T11-W on root-knot nematode egg and application of trichoderma T11-W

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and identification of root-knot nematode ova parasite

[0036] 1. The isolation steps of strain T11-W are as follows:

[0037] (1) Collection of samples: Cucumber root-knot nematodes were collected at Liying Village, Daddy Town, Shouguang. The root system with root-knot nematodes was dug out with a shovel, placed in a kraft paper envelope, and brought back to the laboratory.

[0038] (2) Separation method:

[0039] The collected cucumber root-knot nematode (identified as root-knot nematode incognita Meloidogyne incognita ) diseased root, after washing the root topsoil, cut the root with a large number of root knots, cut it into 1cm root segment, and put it into a 1000ml beaker. Add 500ml of 1% NaClO solution to the beaker, cover and stir for 2 minutes, pour the root fragments together with the solution into the 75 μm and 26 μm sieves, rinse the root fragments on the 75 μm mesh sieve with tap water, so that the root fragments on the 26 μm sieve are...

Embodiment 2

[0045] Example 2 Study on Parasitism of Tomato Meloidogyne Eggs by T11-W

[0046] 1 Preparation of Trichoderma spore suspension

[0047] Trichoderma strains T11-w and LTR-2 were respectively inoculated on PDA medium, cultured at 25°C until conidia were produced, washed with sterile water, and filtered through 5 layers of sterile gauze. Count the spore concentration of the suspension under a microscope with a hemocytometer and dilute it to a target concentration of 7 x 10 7 cfu / mL, spare.

[0048] Preparation of 2 root knot nematode egg suspensions

[0049] Take the tomato root system with severe disease, rinse it with tap water, cut it into 1cm root segments, and put it into a 1000ml beaker. Add 500ml of 1% NaClO solution to the beaker, cover and stir for 2 minutes, pour the root fragments together with the solution into the 75 μm and 26 μm sieves, rinse the root fragments on the 75 μm mesh sieve with tap water, so that the root fragments on the 26 μm sieve are as close as...

Embodiment 3

[0058] The cultivation of embodiment 3 Trichoderma T11-W conidia

[0059] The cultivation of Trichoderma T11-W conidia is carried out in the following steps:

[0060] (1) Incline strains: use solid PDA medium to inoculate Trichoderma T11-W on test tube medium, and culture at 28°C for 2-3 days.

[0061] (2) Eggplant bottle strains: Use liquid PDA medium to inoculate the test tube strains in liquid eggplant bottles, and place them on a shaker at 28°C for shaking culture for 3-4 days.

[0062] (3) Solid inoculation:

[0063] (3.1) Preparation of solid medium: Mix bran and rice husk evenly at a volume ratio of 4:1, then add 50%-60% water, sterilize at 121°C for 40 minutes, and set aside.

[0064] (3.2) Inoculation: Use a mixing inoculator to mix the liquid strain and the solid medium evenly, the inoculum amount is 5-10%, and transfer it to the solid culture room for cultivation after inoculation.

[0065] (4) Cultivation: The thickness of the medium is 5cm, the material tempera...

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Abstract

The invention provides an application of a T11-W bacterial strain in agriculture, and particularly relates to the application of the T11-W bacterial strain in the prevention and cure of soil-borne nematodiasis, especially root-knot nematode diseases of vegetables. The T11-W bacterial strain can be used for effectively preventing and controlling the root-knot nematode diseases of tomatoes. Whether the T11-W bacterial strain is subjected to soil-mixed treatment or root-irrigation treatment, when the effective spore amount each pot is 2*10<7>, the prevention and control effect of the T11-W bacterial strain is above 77%. For the root-irrigation treatment, when the effective spore amount each pot is 2*10<8>, the prevention and control effect of the T11-W bacterial strain reaches up to 90.37%.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the parasitism of Trichoderma T11-W on root-knot nematode eggs and its application. Background technique [0002] Plant parasitic nematodes are an important class of plant pathogens, and their harm exceeds that of bacteria and viruses, and is second only to fungal diseases. It is estimated that the global annual loss due to plant-parasitic nematodes is 125 billion US dollars, and 70% of this loss is caused by root-knot nematodes ( Meloidogyne spp.) caused by. Root-knot nematode has a wide range of hosts and strong fecundity. After infecting crops, it can cause a substantial reduction in crop yield, or even extinction. It has become one of the outstanding problems restricting the sustainable development of agriculture in my country. Therefore, the prevention and control of root-knot nematode has been paid more and more attention by people. However, due to the frequent use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/885
Inventor 王贻莲杨合同李纪顺李哲陈凯魏艳丽吴晓青
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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