Composite microbial agent for prevention and treatment of tomato root knot nematode disease and application thereof

A compound microbial inoculant, tomato root-knot nematode technology, applied in the directions of microorganisms, biocides, nematicides, etc., can solve the problems of single strain, can not further improve the control efficiency, single biocontrol mechanism, etc., to reduce damage and Suppressing, beneficial to human health, effects that do not require special equipment

Inactive Publication Date: 2015-09-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Paecillin lilacin, Puccinia pachytansporum, Abamectin, Bacillus, etc. are more common, and the control efficiency is about 40%-60%. Single, leading to a single biological control mechanism, no synergistic effect between strains, and no further improvement in control efficiency

Method used

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  • Composite microbial agent for prevention and treatment of tomato root knot nematode disease and application thereof
  • Composite microbial agent for prevention and treatment of tomato root knot nematode disease and application thereof
  • Composite microbial agent for prevention and treatment of tomato root knot nematode disease and application thereof

Examples

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Effect test

Embodiment 1

[0026] The invention relates to a composite microbial agent, the active ingredients of which are composed of Glomus versiforme (G.v) and Bacillus subtilis (BS).

[0027] A biocontrol bacterial agent for preventing and treating tomato root-knot nematode is prepared by using the composite microbial bacterial agent.

[0028] The method for the prevention and treatment of tomato root-knot nematode by biocontrol agent, at first, in the tomato seedling cultivation stage, inoculate Glomus G.v in the seedling cultivation soil, the inoculation dose is 2000IPU / strain; Again, in the tomato 3-4 leaf transplanting stage , seedling stage and flowering and fruit-setting stage, inoculate described Bacillus subtilis BS in 3 times, and BS inoculation dose is that the concentration of fermented liquid is 10 9 cfu / ml, 200ml / plant. The inoculation method of Bacillus subtilis is to make 4 small holes in the tomato root circle, and use the root irrigation method to inoculate.

Embodiment 2

[0030]The invention relates to a composite microbial agent, the active ingredients of which are composed of Glomus versiforme (G.v) and Bacillus subtilis (BS).

[0031] A biocontrol bacterial agent for preventing and treating tomato root-knot nematode is prepared by using the composite microbial bacterial agent.

[0032] The method for the prevention and treatment of tomato root-knot nematode by biocontrol agent, at first, in the tomato seedling cultivation stage, inoculate Glomus surface G.v in the seedling cultivation soil, the inoculation dosage is 1000IPU / strain; Again, in the tomato 3-4 leaf transplanting stage , seedling stage and flowering and fruit-setting stage, inoculate described Bacillus subtilis BS in 3 times, and BS inoculation dose is that the concentration of fermented liquid is 10 9 cfu / ml, 500ml / plant. The inoculation method of Bacillus subtilis is to make 4 small holes in the tomato root circle, and use the root irrigation method to inoculate.

Embodiment 3

[0034] The invention relates to a composite microbial agent, the active ingredients of which are composed of Glomus versiforme (G.v) and Bacillus subtilis (BS).

[0035] A biocontrol bacterial agent for preventing and treating tomato root-knot nematode is prepared by using the composite microbial bacterial agent.

[0036] The method for the prevention and treatment of tomato root-knot nematode by biocontrol agent, at first, in the tomato seedling cultivation stage, inoculate Glomus surface G.v in the seedling cultivation soil, the inoculation dose is 5000IPU / strain; , seedling stage and flowering and fruit-setting stage, inoculate described Bacillus subtilis BS in 3 times, BS inoculation dosage is fermented liquid 10 9 cfu / ml, 300ml / plant. The inoculation method of Bacillus subtilis is to make 4 small holes in the tomato root circle, and use the root irrigation method to inoculate.

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Abstract

The invention relates to the technical field of agricultural crop disease prevention and treatment, and in particular, relates to a composite microbial agent for prevention and treatment of tomato root knot nematode disease and an application thereof; a tomato symbiotic AM fungus (glomus versiforme) and plant growth-promoting rhizobacteria PGPR (bacillus subtilis) are used together, and the synergistic effect of the two microorganisms is utilized; compared with a prevention and treatment effect of a general single inoculum or rhzomorph, the prevention and treatment rate reaches 60-70% and is higher than that of the single inoculum or rhzomorph. At the same time, tomato plants applied with the composite microbial agent have developed root systems and especially have more fibrous root systems, and the absorption area of tomato is effectively increased.

Description

technical field [0001] The invention relates to the technical field of crop disease prevention and treatment, in particular to a compound microbial bacterial agent for preventing and treating tomato root-knot nematode and its application. Background technique [0002] Root-knot nematode disease is a kind of worldwide important plant nematode disease caused by root-knot nematode (Meloidogyne spp.), and it is one of the important diseases of vegetables (tomato, cucumber, pumpkin, green pepper, etc.) everywhere. At present, there are more than 90 kinds of root-knot nematodes reported in the world, which mainly parasitize on more than 3,000 hosts such as vegetables, food crops, cash crops, fruit trees and weeds. Plants in temperate, subtropical, and tropical regions are particularly damaged. According to statistics in 2000, the annual losses caused by nematode damage to food and fiber crops in the world are about 12.7%, and the losses caused to vegetables, peanuts, tobacco and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/02A01P5/00C12R1/125C12R1/645
CPCA01N63/00A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 刘润进唐超李敏
Owner QINGDAO AGRI UNIV
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