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Application of bacillus cereus BCJB01 in control of root-knot nematodes

A technology of Bacillus cereus and root-knot nematode, applied in the direction of application, nematicides, bacteria, etc.

Active Publication Date: 2016-06-15
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effectiveness of existing biocontrol bacteria in controlling root-knot nematodes still needs to be improved, and new biocontrol bacteria also need to continue to be developed.

Method used

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  • Application of bacillus cereus BCJB01 in control of root-knot nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation and Identification of BCJB01 Strain

[0026] 1. The present invention relates to the BCJB01 bacterial strain, which is lethal to both the second-instar larvae of tomato root-knot nematode and the eggs about to hatch out of the second-instar larvae, and is isolated from the cabbage field soil in Gaotang County, Liaocheng City, Shandong Province. The strain preservation number is CGMCCNO.10477.

[0027] 2. Strain identification

[0028] The strain BCJB01 was identified as Bacillus cereus by the combination of physiological and biochemical characteristics determination and 16S rDNA sequence analysis. At the same time, isolate and purify the single colony at the same time V. Identification of seven important characteristics including P test, bacterial film formation test, lecithin enzyme test, starch hydrolysis test, casein hydrolysis test, escin, cellobiose, etc. Handbook of Bacterial System Identification.

Embodiment 2

[0030] Lethal effect of BCJB01 on the second instar larvae of tomato root-knot nematode

[0031] 1. The preparation of BCJB01 fermented liquid is carried out according to the following steps:

[0032] (1) Incline strains: use solid LB medium to inoculate Bacillus cereus BCJB01 on test tube medium, and culture at 30°C for 24 hours.

[0033] (2) Shake flask strains: use liquid LB medium, inoculate the test tube strains in the liquid LB medium, place on a shaker at 30°C, and shake for 15 hours.

[0034] (3) Liquid fermentation: medium, 2% corn flour, 1% soybean meal, 0.3% yeast powder, CaCl 2 0.1%, MgSO 4 ·7H 2 O0.035%, Na 2 HPO 4 12H 2 O0.3%, NaH 2 PO 4 2H 2 O0.15%, pH7.0~7.2. Sterilize at 121°C for 20 minutes, inoculate the seeds of (2) in a 10-liter fermenter (the inoculum size is 5%wt), cultivate at 30°C, and dissolve oxygen at 20% , the ventilation volume is 8-12m3 / hour, the tank pressure is 0.05-0.1KPa, the stirring speed is 110RPM, and the cultivation time is 36-...

Embodiment 3

[0044] Research and Application of BCJB01 Preparation

[0045] In the present invention, potassium chloride or ammonium sulfate is used as suspension aid, sodium diacetate is used as preservative, organic silicon BD-3077 is used as surfactant, xanthan gum is used as thickener, and BCJB01 is made into suspending agent. 5×10 9 cfu mL -1 .

[0046] In the present invention, the mixture of white carbon black, glycerin and fatty alcohol polyoxyethylene ether is used as a spray protection agent, BCJB01 is made into mother powder, and then peat soil is used as a carrier, chitosan is used as a synergist, and fatty alcohol polyoxyethylene ether as a wetting agent, 2-naphthalenesulfonic acid formaldehyde polymer sodium salt as a dispersant, and a fluorescent whitening agent CBS as a UV protection agent. The BCJB01 master powder is made into a powder, and the number of effective cells is 7.0×10 8 cfu·g -1 .

[0047] Prevention and treatment of root-knot nematode of tomato by BCJB01 ...

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Abstract

The invention discloses an application of bacillus cereus BCJB01 in control of root-knot nematodes. A supernatant and a bacterial suspension of the bacillus cereus BCJB01 have a completely lethal effect on second instar larvae of tomato root-knot nematodes and eggs being about to hatch out the second instar larvae. One new strain for controlling the root-knot nematodes is provided, the effect of the bacillus cereus BCJB01 is not lower than that of an existing chemical agent in the market, and great progress in the aspect of control of the root-knot nematodes is achieved.

Description

technical field [0001] The invention relates to the application field of bacterial strains, in particular to the application of bacillus cereus BCJB01 in preventing and controlling root-knot nematodes. Background technique [0002] Root-knot nematode is a serious plant nematode parasitic disease, often causing serious economic losses. The control of root-knot nematode disease is an urgent problem to be solved in agricultural production. Traditional control measures mainly include chemical control, crop rotation and use of disease-resistant varieties, but these control measures have their limitations. The use of microorganisms to control root-knot nematodes has the advantage of sustainable development and is one of the ideal ways for sustainable management of root-knot nematode diseases. [0003] Research reports on the use of biocontrol bacteria to control root-knot nematodes mainly include rhizosphere growth-promoting bacteria, Pasteurella puncture, Pseudomonas, Bacillus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P5/00C12N1/20C12R1/085
CPCA01N63/10C12N1/20
Inventor 王贻莲李纪顺谢雪迎陈凯杨合同扈进冬
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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