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Root-knot nematode biocontrol bacterium, antibiological inoculant and application of biocontrol bacterium and antibiological inoculant

A root-knot nematode and biocontrol fungicide technology, applied in the direction of nematicides, applications, bacteria, etc., to achieve low cost and high control efficiency

Active Publication Date: 2015-04-01
广西泰宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The nematicidal properties of this microorganism have not been reported

Method used

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  • Root-knot nematode biocontrol bacterium, antibiological inoculant and application of biocontrol bacterium and antibiological inoculant
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  • Root-knot nematode biocontrol bacterium, antibiological inoculant and application of biocontrol bacterium and antibiological inoculant

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Embodiment 1

[0026] Embodiment 1: the isolation of Providencia rettgeri (Providencia rettgeri) bacterial strain UN06 bacterial strain

[0027] The present inventor isolated and purified a batch of endophytic bacteria from the stems of tomato in Shouguang greenhouse in Shandong Province using a selective medium, and screened out Providence retwie which can effectively kill root-knot nematodes after bioassays on root-knot nematodes. Providencia rettgeri strain UN06. The strain was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on December 26, 2014 (Address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing), and the preservation number: CGMCC No.10246 .

[0028]The biological characteristics of the bacterial strain are as follows: the colonies on the AMS medium plate are milky white, 2 mm in diameter, translucent, round and smooth. The bacteria are rod-shaped, with a ...

Embodiment 2

[0030] Embodiment 2: the preparation of Providencia rettgeri (Providencia rettgeri) bacterial strain UN06 biocontrol agent

[0031] Beef extract peptone solid medium, the formula is: beef extract 5g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH 7.2-7.4;

[0032] Beef extract peptone liquid medium, the formula is: beef extract 5g, peptone 10g, NaCl 5g, water 1000mL, pH7.2-7.4;

[0033] SRC medium, the formula is: sucrose 12.5g, rice flour 50g, crab shell powder 37.5g, NaCl 2.5g, MgSO 4 1.25g, KH 2 PO 4 2.5g, add water to make up to 1000ml, pH 6.5.

[0034] The preparation method specifically comprises the following steps:

[0035] (1) Cultivation of slant seeds: the thallus of the bacterial strain was moved to the slant of beef extract peptone solid medium, and cultivated at 36° C. for 2 days to obtain slant seeds;

[0036] (2) Liquid seed culture: inoculate the slant seeds into the beef extract-peptone liquid medium in the triangular flask, and culture on a shaker at ...

Embodiment 3

[0039] Embodiment 3: Field test of UN06 granule to control greenhouse tomato root-knot nematode

[0040] Test site: vegetable greenhouse in Shouguang City, Shandong Province.

[0041] Experimental crop: tomato (variety: Fendu-80).

[0042] Test agent: a. The number of live bacteria is 3.6×10 9 Each / gram of UN06 granules; b. 2% Abamectin EC.

[0043] Test method: The following three treatments were set up, each treatment had 3 replicates, and each replicate had 120 tomato plants.

[0044] Treatment 1: 100 kg / mu UN06 granules. When preparing the site, spread UN06 granules evenly on the soil surface at an amount of 75 kg / mu, and mix it into the soil with a tiller; apply 10 grams of UN06 granules to the roots when transplanting (according to For 2500 plants, the application rate in holes is 25 kg / mu).

[0045] Treatment 2: Dilute 2% Abamectin EC with water 1000 times for root irrigation.

[0046] Treatment 3: blank control without any nematicide application.

[0047] Invest...

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Abstract

The invention discloses a root-knot nematode biocontrol bacterium, an antibiological inoculant and application of the biocontrol bacterium and the antibiological inoculant. The biocontrol bacterium is a Providencia rettgeri strain UN06, and the preservation serial number of the biocontrol bacterium is CGMCC No. 10246. Fermentation liquor obtained through fermentation of the Providencia rettgeri strain UN06, adsorbing carriers such as mushroom dreg and grass carbon, and nutrient substance urea required by bacterium growth are evenly mixed to be granulated to form UN06 granules. The control efficiencies of 100 kilograms of the applied UN06 granules in each mu to tomato root-knot eelworms, cucumber root-knot nematodes and tobacco root-knot nematodes are 90.17 percent, 88.66 percent and 85.98 percent. The root-knot nematode antibiological inoculant has the advantages of being low in cost, high in control efficiency, free of residual and safe to people and livestock, and is an efficient biopesticide for preventing and treating root knot nematode diseases.

Description

technical field [0001] The invention relates to a root-knot nematode biocontrol bacterium, a biocontrol bacterium and an application thereof in preventing and treating root-knot nematode disease, belonging to the technical field of microbial pesticides. Background technique [0002] Diseases caused by pathogenic nematodes of crops have become the second largest category of diseases in agricultural production. The direct economic loss caused by crop pathogenic nematodes is as high as 157 billion U.S. dollars (Abad P, Gouzy J, Aury JM. et al. 2008. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita. Nat. Biotechnol. 26 (8) :909-15.doi:10.1038 / nbt.1482.), among which the root-knot nematode is the most harmful and causes the greatest loss. Root-knot nematode can harm more than 3,000 kinds of plants such as grain, oilseeds, vegetables, tobacco, and fruits. Root-knot nematodes infect crop roots with second-instar larvae and induce nematode root nodules...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N25/12A01P5/00C12R1/01
CPCA01N25/12A01N63/00C12N1/20C12N1/205C12R2001/01A01N2300/00
Inventor 周峰莫明和潘克静
Owner 广西泰宝生物科技有限公司
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